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2 protocols using anti human max mouse monoclonal

1

Western Blot Analysis of Cell Signaling Proteins

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Western blottng was performed as previously described. Protein was transferred to polyvinylidene diflouride membranes (Millipore) and immunoblotted with anti-human CDK2 rabbit monoclonal, anti-human CDK4 mouse monoclonal, anti-human c-Myc rabbit monoclonal (D84C12), or anti-human p53 mouse monoclonal (clone:1C12) antibodies (all at 1:2000; Cell Signaling). Additional antibodies included anti-human MAX mouse monoclonal (1:2000; Abcam), anti-beta Actin HRP conjugated monoclonal (1:5000; Santa Cruz), or anti-GAPDH HRP conjugated monoclonal antibody (1:2000; Santa Cruz) for at least 1h at room temperature or 4°C overnight. HRP–conjugated goat anti-mouse or anti-rabbit secondary antibodies (Santa Cruz) were added when needed.
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2

Western Blot Analysis of Cell Signaling Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Western blottng was performed as previously described. Protein was transferred to polyvinylidene diflouride membranes (Millipore) and immunoblotted with anti-human CDK2 rabbit monoclonal, anti-human CDK4 mouse monoclonal, anti-human c-Myc rabbit monoclonal (D84C12), or anti-human p53 mouse monoclonal (clone:1C12) antibodies (all at 1:2000; Cell Signaling). Additional antibodies included anti-human MAX mouse monoclonal (1:2000; Abcam), anti-beta Actin HRP conjugated monoclonal (1:5000; Santa Cruz), or anti-GAPDH HRP conjugated monoclonal antibody (1:2000; Santa Cruz) for at least 1h at room temperature or 4°C overnight. HRP–conjugated goat anti-mouse or anti-rabbit secondary antibodies (Santa Cruz) were added when needed.
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