Anti mir 1

The miR-1 mimic, corresponding negative control mimic (ConmiR), the miR-1 inhibitor (Anti-miR-1), control miRNA inhibitor (Control), and the siRNA oligos were purchased from Shanghai GenePharma Co., Ltd. The miRNA-1 mimics were double-stranded siRNA oligos. The sense strand of miRNA-1 mimic (5′-UGGAAUGUAAAGAAGUAUGUAU-3′) consisted of 21 bases, and the antisense strand was complementary to the sense chain. The miR-1 inhibitor consisted of RNA oligos of 21 bases fully complementary to the target sequences and modified with 2′ oxygen methyl. The siRNA oligo for knockdown of Ihh (siIhh) was designed and synthesized by Shanghai GenePharma Co., Ltd., and the sequences were as follows: sense, 5′-CCUUCAGUGAUGUGCUUAUTT-3′.

MiR-1 mimic and its scramble control (miR-NC), miR-1 inhibitor (anti-miR-1) and its scramble control (anti-miR-NC), and small interference RNA targeting MALAT1 (si-MALAT1) and its scramble control (si-NC) were purchased from Gene Pharma Co., Ltd. (Shanghai, China). The sequences of MALAT1 or KRAS were amplified by polymerase chain reaction (PCR) and subcloned into pcDNA3.1 (Thermo Fisher Scientific) to produce MALAT1 or KRAS overexpression plasmid, named as pcDNA-MALAT1 (MALAT1) or pcDNA-KRAS (KRAS). Then, these miRNAs, miRNA inhibitors, siRNAs, or plasmids were transfected into PC cells using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s instructions.