Mangiferin

Mangiferin
(purity ≥98%) was purchased from Chengdu Must Biotechnology
Co., Ltd. (Sichuan, China). HPLC-grade acetonitrile, methanol, and
formic acid were purchased from Thermo Fisher Scientific (Fair Lawn,
NJ, USA). Deionized water used throughout the experiment was purchased
from Watsons (Guangzhou, China). Rat liver microsomes were obtained
from NEWGAINBIO Co., Ltd. (Wuxi, China) and Grace Pure SPE C₁₈-Low SPE cartridges (200 mg/3 mL, 59 μm, 70 Å) were purchased
from Grace Davison Discovery Science (Deerfield, IL, USA). Nicotinamide
adenine dinucleotide phosphate (NADPH) and magnesium chloride (MgCl₂) were provided by Shanghai Macklin Biochemical Co., Ltd.
(Shanghai, China). The 6-well plates were obtained from Corning Incorporated-Life
Science (Jiangsu, China).

C₄H₆O₄Zn•2H₂O was purchased from Shanghai Aladdin Biochemical Technology Co. Ltd. (Shanghai, China). Folin-Ciocalteu's phenol reagent was purchased from Beijing Solarbio Science & Technology Co. Ltd. (Beijing, China). Gallic acid and NaBH₄ were purchased from Sinopharm Chemical Reagent Co. Ltd. (Beijing, China). Phytochemical standards, including mangiferin, rutin, trigonelline, caffeine and chlorogenic acids (5-CQA: 5-caffeoylquinic acid; 3,4-diCQA: 3,4-dicaffeoylquinic acid; 3,5-diCQA: 3,5-dicaffeoylquinic acid; 4,5-diCQA: 4,5-dicaffeoylquinic acid) were purchased from Chengdu Must Bio-Technology Co. Ltd. (Chengdu, China). The fresh leaves of Coffea arabica were obtained from Hougu Coffee Plantation in Mangshi, Dehong County, Yunnan Province, China. All other reagents are analytical grade and purchased from Sinopharm Chemical Reagent Co. Ltd. (Zhenjiang, China).