Cell lysates were prepared using complete lysis buffer (EMD Millipore, San Diego, CA) with protease and phosphatase inhibitor cocktails (Roche Diagnostics, Indianapolis, IN). Protein quantification was performed using Dc protein assay (Bio-Rad, Hercules, CA). Western blot analysis was performed as described previously.16 (link), 17 (link) Antibodies used include Akt1, Akt2, Akt3, pan-Akt, Tyr216GSK-3β, Ser33/37/Thr41β-catenin, claudin-5, ZO-1 and ZO-2 (Cell Signaling) and anti-β-actin (Sigma). Densitometry was done using NIH Image J software.
Ser33 37 thr41β catenin
Manufactured by Cell Signaling Technology
Sourced in United States
Ser33/37/Thr41β-catenin is a phospho-specific antibody that detects β-catenin when phosphorylated at Ser33, Ser37, and Thr41. β-catenin is a key component of the Wnt signaling pathway and plays a role in cell-cell adhesion.
Automatically generated - may contain errors
Lab products found in correlation
Complete lysis buffer, by Merck Group (1 mentions)
Claudin 5, by Cell Signaling Technology (1 mentions)
Pan akt, by Cell Signaling Technology (1 mentions)
Zo 1 and zo 2, by Cell Signaling Technology (1 mentions)
Anti β actin, by Merck Group (1 mentions)
Dc protein assay, by Bio-Rad (1 mentions)
Protease and phosphatase inhibitor cocktail, by Roche (1 mentions)
Total β catenin, by Cell Signaling Technology (1 mentions)
Ser473 akt, by Cell Signaling Technology (1 mentions)
Caspase 3, by Enzo Life Sciences (1 mentions)
α tubulin, by Merck Group (1 mentions)
Image quant las 500 chemiluminescence detection system, by GE Healthcare (1 mentions)
Ser45 β catenin, by Cell Signaling Technology (1 mentions)
Gapdh, by Thermo Fisher Scientific (1 mentions)
Total akt, by Cell Signaling Technology (1 mentions)
Mcl 1, by Cell Signaling Technology (1 mentions)
Quantity one software, by Bio-Rad (1 mentions)
2 protocols using ser33 37 thr41β catenin
1
Western Blot Analysis of Cell Signaling
2
Western Blot Analysis of Cell Signaling
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
WB was performed as described [49 (link)]. Antibodies used were the following: CK1α, PARP, Mcl1, Ser45 β-catenin, Ser33/37/Thr41 β-catenin, total β-catenin, Ser473 AKT, total AKT (Cell signaling Technology, MA, USA); Mdm2 (Millipore, Italy), GAPDH (Ambion, USA); α-tubulin (Sigma-Aldrich, Italy); Bak (Merck, MA, USA); Bax, p21, p53 (Becton Dickinson, Italy); Caspase 3 (Enzo Life Science, UK). Images were acquired using the Image Quant LAS 500 chemiluminescence detection system (GE Healthcare, USA). Densitometric analysis was performed with Quantity One software (Bio-Rad, Italy).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!