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Anti yap sc 101199

Manufactured by Santa Cruz Biotechnology
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Anti-YAP (SC-101199) is a primary antibody product manufactured by Santa Cruz Biotechnology. It is designed to detect the expression of the YAP (Yes-associated protein) protein. YAP is a transcriptional regulator that plays a role in the Hippo signaling pathway, which is involved in the regulation of cell proliferation and organ size.

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Lab products found in correlation

Anti ha mms 101r, by Fortrea (3 mentions) α sma, by Thermo Fisher Scientific (3 mentions) Anti myc sc 40, by Santa Cruz Biotechnology (2 mentions) Anti phospho yap s127 4911s, by Cell Signaling Technology (2 mentions) Anti flag m2 f3165 5mg, by Merck Group (2 mentions) Anti α tubulin t6199, by Merck Group (2 mentions) Anti gapdh gb12002, by Wuhan Servicebio Technology (2 mentions) Anti myc, by Abcam (2 mentions) Anti β actin, by Merck Group (1 mentions) Ab138911, by Abcam (1 mentions) Ab138910, by Abcam (1 mentions) P nitrobenzyl mesylate, by Abcam (1 mentions) Polyvinylidene fluoride pvdf membrane, by Merck Group (1 mentions) Epidermal growth factor (egf), by Merck Group (1 mentions) Anti tead1 antibody, by BD (1 mentions) Anti irβ sc 711, by Santa Cruz Biotechnology (1 mentions) Igf 1, by Thermo Fisher Scientific (1 mentions) Ly294002, by Selleck Chemicals (1 mentions) Hoechst 33342, by BD (1 mentions) Anti hsp90, by Cell Signaling Technology (1 mentions)

Spelling variants of this product

Anti-YAP (50 citations) Sc-101199 (44 citations) YAP (sc-101199, (4 citations) Anti-YAP/TAZ (sc-101199; (4 citations) Mouse anti-YAP (sc-101199, (4 citations)

10 protocols using anti yap sc 101199

1

Western Blot Analysis of Protein Interactions

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Cells were harvested and lysed with RIPA buffer. Proteins were separated by electrophoresis on SDS-polyacrylamide gel electrophoresis (PAGE) and electro-transferred to PVDF membrane. The antibodies used in this study were listed here: Anti-RNF187 (HAP030098, Sigma); Anti-YAP (SC-101199, Santa Cruz); Anti-HA (MMS-101R, COVANCE); Anti-myc (9E10, ab32, Abcam); Anti-myc (Ab9106, Abcam); Anti-GAPDH (GB12002, Servicebio); Anti-Flag (20543-1-AP, Proteintech); Anti-GFP (Ab290, Abcam). For western blot assays, the antibodies were used in the following concentration: Anti-YAP (SC-101199, Santa Cruz): 1/2000; Anti-HA (MMS-101R, COVANCE): 1/1000; Anti-myc (9E10, ab32, Abcam): 1/1000; Anti-RNF187 (HAP030098, Sigma): 1/1000; Anti-Flag (20543-1-AP, Proteintech): 1/1000; Anti-GFP (Ab290, Abcam): 1/1000. Membranes were then washed with PBS for three times and incubated with secondary antibodies Peroxidase-Conjugated AffiniPure Goat Anti-Mouse IgG or Goat Anti-Rabbit IgG. Fluorescent signals were visualized with ECL system. (amersham imager 600, USA).
Wang Z., Kong Q., Su P., Duan M., Xue M., Li X., Tang J., Gao Z., Wang B., Li Z., Liu Y., Yang X., Cao R., Song T., Wang K., Cai Y., Wu D., Li J., Wu G., Guled A.M., Zhu J., Yan C, & Zhuang T. (2020). Regulation of Hippo signaling and triple negative breast cancer progression by an ubiquitin ligase RNF187. Oncogenesis, 9(3), 36.
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2

Antibody Characterization for Western Blotting, Immunostaining, and IHC

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For western blotting, anti-Flag (M2) (F3165–5MG, 1:5000 dilution), anti-α-tubulin (T6199–200UL, 1:5000 dilution) and anti-β-actin (A5441–100UL, 1:5000 dilution) monoclonal antibodies were obtained from Sigma-Aldrich. An anti-Myc (sc-40, 1:500 dilution) antibody was purchased from Santa Cruz Biotechnology. An anti-hemagglutinin (HA) monoclonal antibody (MMS-101P, 1:3000 dilution) was obtained from Covance. An anti-STRN4 (A304–573A-T, 1:1000 dilution) was obtained from Bethyl laboratories. Anti-phospho-YAP (S127) (4911S, 1:1000 dilution), anti-phospho-LATS1 (T1079) (8654S, 1:1000 dilution) and anti-LATS1 (3477S, 1:1000 dilution) antibodies were purchased from Cell Signaling Technology. The YAP polyclonal antibody was raised against a GST-YAP fusion protein and the antisera were affinity-purified using an AminoLink Plus Immobilization and Purification Kit (Pierce) (Wang et al., 2011 (link)).
For immunostaining, an anti-YAP (sc-101199, 1:200 dilution) monoclonal antibody was purchased from Santa Cruz Biotechnology. An anti-HA polyclonal antibody (3724S, 1:3000 dilution) was obtained from Cell Signaling Technology.
For immunohistochemical staining, an anti-YAP (14074S, 1:20 dilution) monoclonal antibody was purchased from Cell Signaling Technology. An anti-STRN4 (A304–573A-T, 1:20 dilution) was obtained from Bethyl laboratories.
Seo G., Han H., Vargas R.E., Yang B., Li X, & Wang W. (2020). MAP4K Interactome Reveals STRN4 as a Key STRIPAK Complex Component in Hippo Pathway Regulation. Cell reports, 32(1), 107860.
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3

Western Blot Antibody Characterization

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For Western blotting, anti-α-tubulin (T6199, 1:5000 dilution) and anti-Flag (M2) (F3165-5MG, 1:5000 dilution) antibodies were obtained from Sigma-Aldrich. Anti-Myc (sc-40, 1:500 dilution) and anti-GST antibodies (sc-138, 1:1000 dilution) were purchased from Santa Cruz Biotechnology. Anti-phospho-YAP (S127) (4911S, 1:1000 dilution) and anti-phospho-LATS1 (Thr1079) (8654S, 1:1000 dilution) antibodies were purchased from Cell Signaling Technology. Anti-hemagglutinin (HA) monoclonal antibody (901514, 1:2000 dilution) was obtained from BioLegend. Anti-Thiophosphate ester antibody (ab92670, 1:1000 dilution) was purchased from Abcam. The YAP and MBP polyclonal antibodies were generated as previously described57 (link),61 (link). ATP-γ-S kinase substrate (ab138911) and p-Nitrobenzyl mesylate (ab138910) were obtained from Abcam. For immunostaining, an anti-YAP (sc-101199, 1:200 dilution) monoclonal antibody was purchased from Santa Cruz Biotechnology. Anti-Flag polyclonal antibody (F7425, 1:5000 dilution) was obtained from Sigma-Aldrich.
Chen Y., Han H., Seo G., Vargas R.E., Yang B., Chuc K., Zhao H, & Wang W. (2020). Systematic analysis of the Hippo pathway organization and oncogenic alteration in evolution. Scientific Reports, 10, 3173.
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4

Insulin and Growth Factor Signaling Analysis

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HepG2 and C2C12 cells were obtained from the American Type Culture Collection. All reagents for tissue culture were from Life Technologies. Polyvinylidene fluoride (PVDF) membranes were purchased from Millipore Corp. Anti-YAP (#12395 and #14074), anti-pYAP Ser127 (#13008), anti-histone H3 (#9715), anti-phospho-tyrosine (#8954), anti-IR (#3020), and anti-HSP90 (#4877) antibodies were purchased from Cell Signaling. Anti-TEAD1 antibody (#610922) was from BD Biosciences. Anti-IRβ (sc-711) and anti-YAP (sc-101199) were from Santa Cruz Biotechnology. Hoechst 33342 (#561908) was purchased from BD Pharmingen. Blocking buffer and infrared dye–conjugated (IRDye) antibodies, both anti-mouse and anti-rabbit, were obtained from LI-COR Biosciences. Insulin (#I9278-5ML) and EGF (#SRP3027-500UG) were purchased from Sigma. IGF-1 was purchased from Fisher Scientific (291G1200). Control (AM4635) and YAP (107951) siRNA were from ThermoFisher. LY294002 (#S1105) was from Selleckchem. Charcoal-stripped fetal bovine serum (chFBS) was purchased from Thermo Fisher Scientific (#12676029).
Sayedyahossein S., Hedman A.C, & Sacks D.B. (2020). Insulin suppresses transcriptional activity of yes-associated protein in insulin target cells. Molecular Biology of the Cell, 31(2), 131-141.
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5

Protein Extraction and Western Blot Analysis

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Cells were harvested and lysed with RIPA buffer. Proteins were separated by electrophoresis on SDS-polyacrylamide gel electrophoresis (PAGE) and electro-transferred to PVDF membrane. The antibodies used in this study were listed here: Anti-SHARPIN alpha (Ab125188, Abcam); Anti-YAP (SC-101199, Santa Cruz); Anti-HA (MMS-101R, COVANCE); Anti-myc (9E10, ab32, Abcam); Anti-myc (Ab9106, Abcam); Anti-GAPDH (GB12002, Servicebio). Membranes were then washed with PBS for three times and incubated with secondary antibodies Peroxidase-Conjugated AffiniPure Goat Anti-Mouse IgG or Goat Anti-Rabbit IgG. Fluorescent signals were visualized with ECL system. (amersham imager 600, USA).
Zhang A., Wang W., Chen Z., Pang D., Zhou X., Lu K., Hou J., Wang S., Gao C., Lv B., Yan Z., Chen Z., Zhu J., Wang L., Zhuang T, & Li X. (2019). SHARPIN Inhibits Esophageal Squamous Cell Carcinoma Progression by Modulating Hippo Signaling. Neoplasia (New York, N.Y.), 22(2), 76-85.
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6

Antibody Characterization for Yap, GAPDH, and Caspase 8

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Anti-Yap (SC-101199) and anti-GAPDH (SC-47724) antibodies were from Santa Cruz Biotechnology (Dallas, TX, USA). Anti-caspase 8 (1C12) (9746), was purchased from Cell Signaling Technology (Beverly, MA, USA). Anti-cFLIP monoclonal antibody 7F10 (ALX-804-961-0100) was from Enzo Life Sciences (Farmingdale, NY, USA). Horseradish peroxidase-conjugated secondary antibodies were purchased from DAKO (P0447, P0448, P0449) (Cambridge, UK). Alexa 488-conjugated secondary antibody was obtained from Jackson ImmunoResearch (Baltimore Pike, PA, USA).
El Yousfi Y., Mora-Molina R., López-Rivas A, & Yerbes R. (2023). Role of the YAP/TAZ-TEAD Transcriptional Complex in the Metabolic Control of TRAIL Sensitivity by the Mevalonate Pathway in Cancer Cells. Cells, 12(19), 2370.
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7

Immunoblotting analysis of RNF181, YAP, and GAPDH

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RIPA buffer lysates harvest the cells. Proteins were separated by electrophoresis on SDS-polyacrylamide gel electrophoresis (PAGE) and electro-transferred to PVDF membrane. The antibodies we use include: Anti-RNF181 (SAB1401685, Sigma); Anti-YAP (SC-101199, Santa Cruz); Anti-phospho-YAP (S127) (ab76252, Abcam); Anti-HA (MMS-101R, COVANCE); Anti-myc (9E10, ab9106, Abcam); Anti-GAPDH (GB12002, Servicebio). Membranes were then washed with PBS for three times and incubated with secondary antibodies Peroxidase-Conjugated AffiniPure Goat Anti-Mouse IgG or Goat Anti-Rabbit IgG. Fluorescent signals were visualized with ECL system (GE RRPN 2235).
Zhou R., Ding Y., Xue M., Xiong B, & Zhuang T. (2020). RNF181 modulates Hippo signaling and triple negative breast cancer progression. Cancer Cell International, 20, 291.
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8

Immunoprecipitation of YAP and RACO-1

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IP lysate (containing protease inhibitors) was used to extract cell protein. The EC9706 cells proteins were immunoprecipitated with Protein A/G Agarose beads (P2006, Beyotime) and Anti‐YAP (SC‐101199, Santa Cruz)/Anti‐RACO‐1 (HAP030098, Sigma) or Anti‐IgG antibodies overnight at 4°C and detected by the Western blot.
Pang D., Wang W., Zhou X., Lu K., Zhang J., Chen Z., Wang L., Shen F., Chen Z., Wang S., Hou J., Zhang A., Lv B., Gao C., Yan Z., Hu Y., Chang T., Wang L, & Li X. (2020). RACO‐1 modulates Hippo signalling in oesophageal squamous cell carcinoma. Journal of Cellular and Molecular Medicine, 24(20), 11912-11921.
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9

Antibody Immunoblot Analysis

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The antibodies in this study included anti-α-tubulin (DM1A; Sigma), anti-Erk1/2 (137F5; Cell Signaling Technology), anti-Phospho-Erk1/2 (D13.14.4E; Cell Signaling Technology), anti-Cofilin (ACFL02; Cytoskeleton), anti-Phospho-Cofilin (Ser3) (#3311; Cell Signaling Technology), and anti-YAP (sc101199; Santa Cruz) (detects both YAP and TAZ).
Ito T., Taniguchi H., Fukagai K., Okamuro S, & Kobayashi A. (2015). Inhibitory Mechanism of FAT4 Gene Expression in Response to Actin Dynamics during Src-Induced Carcinogenesis. PLoS ONE, 10(2), e0118336.
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10

Monoclonal Antibody Sourcing Protocol

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Monoclonal antibodies were sourced as follows: anti-YAP (sc-101199) and anti-TEF-1 (sc-376113) from Santa Cruz Biotechnology; anti-CAV1 XP (#3267) and anti-YWHAH (14-3-3 η (D23B7); #5521) from Cell Signaling (Danvers, Massachusetts, United States); anti-Flag M2 (F-3167) from Sigma-Aldrich; and anti-glyceraldehyde-3-phosphate dehydrogenase (MAB374) and anti-cortactin (p80/85, clone 4F11) from Millipore (Burlington, Massachusetts, United States). Polyclonal antibodies to phospho-YAP (Ser127) (#9411), LATS1 (#9153), and LATS2 (#13646) were from Cell Signaling; anti-Histone H3 (ab1791) was from Abcam (Cambridge, United Kingdom); and anti-RHO GDI (sc-360) was from Santa Cruz Biotechnology. For immunohistochemistry, we used anti-YAP (D8H1X) XP from Cell Signaling (#14074) and αSMA from Thermo Fisher Scientific (RB-9010-P0).
Moreno-Vicente R., Pavón D.M., Martín-Padura I., Català-Montoro M., Díez-Sánchez A., Quílez-Álvarez A., López J.A., Sánchez-Álvarez M., Vázquez J., Strippoli R, & del Pozo M.A. (2018). Caveolin-1 Modulates Mechanotransduction Responses to Substrate Stiffness through Actin-Dependent Control of YAP. Cell Reports, 25(6), 1622-1635.e6.
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