Log In Sign up
The largest database of trusted experimental protocols

PM054 is a laboratory equipment product manufactured by Santa Cruz Biotechnology. It is designed to perform a core function within the scientific research environment. The detailed specifications and intended use of this product are not included in this response.

Automatically generated - may contain errors

Lab products found in correlation

Ab124267, by Abcam (1 mentions) Sc 23950, by Merck Group (1 mentions) Sc 33958, by Santa Cruz Biotechnology (1 mentions) Af 401 na, by Santa Cruz Biotechnology (1 mentions) F1804, by Merck Group (1 mentions) Ab9194, by Abcam (1 mentions) Sc 56, by Santa Cruz Biotechnology (1 mentions) Ab181047, by Fortis Life Sciences (1 mentions)

2 protocols using pm054

1

Immune Cell Characterization in Mouse Peritoneal Exudates

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies against NLRP3 (Adipogen, Cryo2) (Sigma, HPA012878), IL-1β (R&D systems, AF-401-NA), caspase-1 (Santa Cruz, SC-154), MAP1 (Sigma, HM-1), NEK7 (Abcam, ab133514), MAP4 (BD, 611026), α-tubulin (MBL, PM054), acetylated tubulin (Santa Cruz, SC-23950), γ-tubulin (Sigma, T5326), Tom20 (Santa Cruz, FL-145, SC-11451), ERK2 (Santa Cruz, C-14), HA tag (Santa Cruz, y-11), Flag tag (Sigma, F1804) were used for western blots. Antibodies against MARK4 (Cell Signaling, 4834) (Abcam, ab124267) (house-made by MRC-PPU, the University of Dundee, UK), NLRP3 (Sigma, HPA012878; Abcam, ab4207), ASC (Enzo, ADI-905-173) (Santa Cruz, SC-33958) were used for in situ proximity-ligation assay. Antibodies against markers CD11b (BIOLEGEND, 101217, 1 in 800 dilution), F4/80 (ebiosciences, 25-4801-82, 1 in 200 dilution), Ly6G (BD, 551461, 1 in 400 dilution), and Ly6C (AbD SeroTec, mca771a647, 1 in 400 dilution) were used for flow cytometry. See Supplementary Fig. 15d for gating strategy for peritoneal exudates.
Li X., Thome S., Ma X., Amrute-Nayak M., Finigan A., Kitt L., Masters L., James J.R., Shi Y., Meng G, & Mallat Z. (2017). MARK4 regulates NLRP3 positioning and inflammasome activation through a microtubule-dependent mechanism. Nature Communications, 8, 15986.
+ Open protocol
+ Expand
2

Antibody Panel for Chromatin Factors

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies used in this work were as below: ESCO1 (Abcam, ab180100), ESCO2 (Abcam, ab86003), CUL4A (proteintech, 14851-1-AP), CUL4B (Proteintech, 12916-1-AP), DDB1 (Abcam, ab9194), MMS22L (Abcam, ab181047), SMC3 (BETHYL, A300-060A), acetylated SMC3 (Merck, MABE1073), Orc2 (CST, #4736), Tubulin (MBL, PM054) and PCNA (Santa Cruz, sc-56).
Sun H., Zhang J., Xin S., Jiang M., Zhang J., Li Z., Cao Q, & Lou H. (2019). Cul4-Ddb1 ubiquitin ligases facilitate DNA replication-coupled sister chromatid cohesion through regulation of cohesin acetyltransferase Esco2. PLoS Genetics, 15(2), e1007685.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!