Von willebrand factor vwf

Manufactured by Santa Cruz Biotechnology

Sourced in United Kingdom, United States

Von Willebrand factor (vWF) is a large glycoprotein that plays a crucial role in the blood coagulation process. It is responsible for the initial adhesion of platelets to the site of vascular injury, thus promoting the formation of a stable platelet plug. vWF also acts as a carrier for factor VIII, a key component of the coagulation cascade.

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Lab products found in correlation

Lsm 780, by Zeiss (1 mentions) Alexa fluor 488 or 555 conjugated secondary antibody, by Thermo Fisher Scientific (1 mentions) Nestin, by Santa Cruz Biotechnology (1 mentions) 4 6 diamino 2 phenylindole dapi, by LGC (1 mentions) Trypsin edta, by Thermo Fisher Scientific (1 mentions) Penicillin streptomycin, by Merck Group (1 mentions) Ascorbic acid 2 phosphate, by Merck Group (1 mentions) Gelatin type b, by Merck Group (1 mentions) Phosphate buffered saline (pbs), by Thermo Fisher Scientific (1 mentions) Alexa fluor 546, by Thermo Fisher Scientific (1 mentions) Fibronectin, by Merck Group (1 mentions) Trypan blue, by Merck Group (1 mentions) Amphotericin b, by Merck Group (1 mentions) Oil red o, by Merck Group (1 mentions)

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Von Willebrand factor (6 citations)

2 protocols using von willebrand factor vwf

Multifaceted Characterization of Brain Cells

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Brain sections were immunostained with antibodies against Tuj1 (Stemcell Technologies, Vancouver, BC, Canada), microtubule-associated protein 2 (MAP2; Sigma, St. Louis, MO), glial fibrillary acidic protein (GFAP; Millipore, Temecula, CA), S-100β (Abcam, Cambridge, United Kingdom), nestin (Santa Cruz Biotechnology, Dallas, TX), von Willebrand factor (vWF; Santa Cruz Biotechnology), alpha smooth muscle actin (αSMA; LifeSpan Biosciences, Seattle, WA), and neuron-glial antigen 2 (NG2; Millipore). Primary antibodies were visualized using Alexa Fluor 488- or 555-conjugated secondary antibodies (Molecular Probes, Eugene, OR). Nuclei were counterstained with 4′,6-diamino-2-phenylindole (DAPI; Kirkegaard & Perry Laboratories, Gaithersburg, MD). Images were captured using a confocal laser microscope (LSM780; Carl Zeiss, Jena, Germany).

Tatebayashi K., Tanaka Y., Nakano-Doi A., Sakuma R., Kamachi S., Shirakawa M., Uchida K., Kageyama H., Takagi T., Yoshimura S., Matsuyama T, & Nakagomi T. (2017). Identification of Multipotent Stem Cells in Human Brain Tissue Following Stroke. Stem Cells and Development, 26(11), 787-797.

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Adipose Cell Culture Protocol

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Dulbecco’s modified Eagle medium-low glucose (DMEM-LG) and 0.25% trypsin-EDTA were from Invitrogen (Carlsbad, CA, USA). Phosphate-buffered saline from Thermo-Fisher Scientific (Waltham, MA, USA). Fetal bovine serum (FBS) was from HyClone (Logan, UT, USA). Penicillin/streptomycin, amphotericin B, ascorbic acid 2-phosphate, trypan blue, fibronectin, gelatin type B from bovine skin, and 1,1,1,3,3,3 hexafluoro-2-propanolol (HFIP) were from Sigma-Aldrich (St Louis, MO, USA). Von Willebrand factor (vWF) from Santa Cruz Biotechnology (Santa Cruz, CA, USA) and anti-human mitochondria (hMito, Abcam, Cambridge, UK) was used as primary antibodies for immunofluorescence. Alexa Fluor 546 (Invitrogen, Carlsbad, CA, USA) was used as secondary antibody. Oil Red O (Sigma-Aldrich) was employed for staining lipid droplets to visualize adipose cells.

Grau-Monge C., Delcroix G.J., Bonnin-Marquez A., Valdes M., Awadallah E.L., Quevedo D.F., Armour M.R., Montero R.B., Schiller P.C., Andreopoulos F.M, & D’Ippolito G. (2017). MIAMI cells embedded within a biologically-inspired construct promote recovery in a mouse model of peripheral vascular disease. Biomedical materials (Bristol, England), 12(1), 015024.

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