Hbv dna test kit

Pregnant women were screened for HBsAg using the ELISA method when they visited the hospital for perinatal health care in China. All the pregnant women who participated in the survey were interviewed and given a questionnaire to fill out. Personal information about the pregnant women, such as age, gestational age, history of therapy, and other factors, were included in the questionnaire. Before delivery, 5 ml of blood sample was taken from the expecting mothers. All serum samples were delivered to the Henan provincial Center for Disease Control and Prevention to test for HBsAg, HBeAg, and HBeAb using ELISA test kits (Beijing Wantai Biological Pharmacy Enterprise Co., Ltd.). The amount of HBV DNA in the collected serum was measured by real-time PCR using the HBV DNA test kit (DAAN Gene Co., Ltd. China).

The enzyme-linked immunosorbent assay (ELISA) was used to measure HBsAg and hepatitis B e antigen (HBeAg) according to the manufacturer’s instructions of HBsAg and HBeAg diagnostic kits (Shanghai Kehua Biotech Co. Ltd., Shanghai, China). The inhibitory level was calculated as: Inhibitory rate (%) = (1 − OD_{IFN-CSP or Mu-IFN-CSP}/OD_control) × 100%. The real-time fluorescent quantification PCR (FQ-PCR) was used to measure the copies of HBV-DNA according to the manufacturer’s recommendations of HBV-DNA test kit (Da-An Gene Co., Guangzhou, China). The inhibitory level was calculated as: Inhibitory rate (%) = (1 − HBV-DNA copies_{IFN-CSP or Mu-IFN-CSP}/HBV-DNA copies_control) × 100%.