At three days after BmNPV infection, 3 mL of hemolymph was collected from pools of twenty BmNPV-infected animals or twenty controls on ice and centrifuged at 500 g for 2 min at 4°C. The hemocyte pellet was washed twice using 1 mL of cold Grace’s insect medium (Gibco, USA) supplemented with 10% FBS (Gibco, USA), which was followed by filtering through a 40 µm cell strainer (Biosharp, China) and centrifugation at 500 g for 2 min at 4°C. The hemocyte pellet was resuspended using 200 µL PBS (calcium and magnesium-free, Gibco, USA) supplemented with 0.04% bovine serum albumin (BSA) (Solarbio, China). Cell viability and number were assessed by 0.4% trypan blue staining and cell counting using a hemocytometer. High quality hemocyte preparations were subjected to single cell encapsulation by 10X Genomics v3 kit (10x Genomics, USA).
40 m cell strainer
Manufactured by Biosharp
Sourced in China
The 40 µm cell strainer is a lab equipment used for filtering and separating cells based on their size. It features a mesh screen with a pore size of 40 micrometers, allowing for the efficient removal of larger cells or cell clumps while allowing smaller cells to pass through.
Automatically generated - may contain errors
Lab products found in correlation
Genomics v3 kit, by 10x Genomics (2 mentions)
Grace s insect medium, by Thermo Fisher Scientific (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Bovine serum albumin (bsa), by Solarbio (2 mentions)
Phosphate buffered saline (pbs), by Thermo Fisher Scientific (1 mentions)
2 protocols using 40 m cell strainer
1
Isolating Hemocytes from BmNPV-Infected Silkworms
2
Transcriptomics of BmNPV-infected Hemocytes
Check if the same lab product or an alternative is used in the 5 most similar protocols
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At three days after BmNPV infection, three mL of hemolymph was collected from pools of twenty BmNPV-infected animals and twenty controls on ice and centrifuged at 500 g for 2 min at 4 °C . The hemocyte pellet was washed twice using 1 mL cold Grace's insect medium (Gibco, USA) supplemented with 10% FBS (Gibco, USA), which was followed by filtering through a 40 µm cell strainer (Biosharp, China) and centrifugation at 500 g for 2 min at 4 °C . The hemocyte pellet was resuspended using 200 µL PBS (calcium and magnesium-free, Gibco, USA) supplemented with 0.04% bovine serum albumin (BSA) (Solarbio, China). Cell viability and number were assessed by 0.4% trypan blue staining and cell counting using a hemocytometer.
High quality single hemocyte preparations were subjected to encapsulation by 10X Genomics v3 kit (10x Genomics, USA).
High quality single hemocyte preparations were subjected to encapsulation by 10X Genomics v3 kit (10x Genomics, USA).
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