Epigallocatechin gallate (egcg)

Sodium dodecyl sulfate (SDS) and bovine serum albumin (BSA) were purchased from Sigma-Aldrich Corp (St. Louis, MO, USA). Cell culture media was obtained from Life Technologies Corp (Carlsbad, CA, USA). Electrophoresis reagents were purchased from Bio-Rad Laboratories (Hercules, CA, USA). The HyGLO™ Chemiluminescent HRP (horseradish peroxidase) Antibody Detection Reagents were from Denville Scientific Inc. (Metuchen, NJ, USA). Micro bicinchoninic acid (BCA) protein assay reagents were from Pierce (Micro BCA™ Protein Assay Kit; Thermo Fisher Scientific, Waltham, MA, USA). The JAK family tyrosine kinase inhibitor AG490 was from Calbiochem (La Jolla, CA, USA). The monoclonal antibodies against GAPDH (D4C6R), pAKT (Ser473) (D9W9U, #12694), caspase-3, and the polyclonal antibodies against PARP (#9542), pSrc (Tyr416, #2101), STAT3 (79D7, #4904), and BCL-2 (50E3, #2870) were all from Cell Signaling Technologies (Danvers, MA, USA). The rabbit polyclonal antibody against CD133 (ab19898) was from Abcam (Toronto, ON, Canada). HRP-conjugated donkey anti-rabbit and anti-mouse immunoglobulin (Ig) G secondary antibodies were from Jackson ImmunoResearch Laboratories (West Grove, PA, USA). EGCG was from MP Biomedicals (Solon, OH, USA). All other reagents were from Sigma-Aldrich Corp.