Perkin elmer spectrum gx

Transmission FTIR spectra were recorded on a PERKIN ELMER SPECTRUM GX (Perkin- Elmer Instruments LLC, Shelton, CT, USA). The KBr sample disk was scanned with a scan number of 8 from 400 to 4000 cm^-1 having a resolution of 4 cm^-1.

Thin layer chromatography (TLC) analysis was performed on TLC silica gel 60 F254 aluminum sheet 20 × 20 cm (Merck, Darmstadt, Germany). A silica gel column (0.040–0.063 mm granule size), a sephadex LH-20 column (particle size dry 18–111 µm), and a C18 column (40–63 particle size) were used for chromatographic isolation of the extract constituents. Fourier-transform infrared (FT-IR) spectra were recorded with attenuated total reflectance (ATR) mode on a PerkinElmer spectrum GX (Perkin Elmer, Waltham, MA, USA). Optical rotations were measured using a POLAX-2L polarimeter (Atago, Japan). An Agilent 1260 infinity high performance liquid chromatography instrument via an electrospray ionization (ESI) interface to a 6540 ultrahigh definition accurate mass Q-TOF (Agilent Technologies, Palo Alto, CA, USA) was conducted. Nuclear magnetic resonance (NMR) spectra were recorded on a Bruker AV400 (Bruker, Billerica, MA, USA) spectrometer at 400 MHz for proton and 100 MHz for carbon. The absorbance was measured using hybrid Multi-Mode Detection Synergy H1 (Model H1MF) (Bio-TeK Instruments, Winooski, VT, USA). The high performance liquid chromatography (HPLC) was performed using Agilent Technology (model 1260 infinity with fraction collector, Santa Clara, CA, USA).