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H 7650 electron microscope

Manufactured by Hitachi
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Sourced in Japan

The Hitachi H-7650 is a transmission electron microscope that provides high-resolution imaging capabilities. Its core function is to magnify and capture images of specimens at the nanometer scale, enabling detailed analysis of the internal structure and composition of materials.

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Lab products found in correlation

Osmium tetroxide, by Electron Microscopy Sciences (9 mentions) Em uc6 ultramicrotome, by Leica camera (7 mentions) Glutaraldehyde, by Electron Microscopy Sciences (7 mentions) Lkb 3 ultratome, by Leica Microsystems (7 mentions) Lead citrate, by Electron Microscopy Sciences (6 mentions) Uranyl acetate, by Electron Microscopy Sciences (6 mentions) Embed 812, by Electron Microscopy Sciences (6 mentions) Luveak 812, by Nacalai Tesque (5 mentions) Ccd xr611 m digital camera, by Advanced Microscopy Techniques (4 mentions) Jem 2010, by JEOL (3 mentions) Paraformaldehyde (pfa), by Electron Microscopy Sciences (3 mentions) 0.05 m sodium cacodylate, by Electron Microscopy Sciences (3 mentions) Lkb 3 ultratome, by Leica camera (2 mentions) Em uc6, by Leica camera (2 mentions) Escalab 250xi xps instrument, by Thermo Fisher Scientific (2 mentions) Uv vis 2450 spectrophotometer, by Shimadzu (2 mentions) Rf 5301pc fluorescence spectrometer, by Shimadzu (2 mentions) Power tome xl ultramicrotome, by Leica camera (2 mentions) S 450 microscope, by Hitachi (2 mentions) Epon embedding, by Merck Group (2 mentions)

172 protocols using h 7650 electron microscope

1

Exosome Morphology Analysis by TEM

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The exosome suspension was placed on a special copper mesh of the H-7650 electron microscope (Hitachi, Tokyo, Japan), followed by negative staining with 2% phosphotungstic acid (20 μL) for 10 min. The morphology of exosomes was observed by the H-7650 electron microscope (Hitachi) at 100 kV.
Yu Y., Bian L., Liu R., Wang Y, & Xiao X. (2021). Circular RNA hsa_circ_0061395 accelerates hepatocellular carcinoma progression via regulation of the miR-877-5p/PIK3R3 axis. Cancer Cell International, 21, 10.
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2

Nanomaterial Characterization by DLS and TEM

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The average particle size (PS), polydispersity index (PDI) and zeta potential (ZP) of NPs were measured by dynamic light scattering (DLS) using a Malvern Zetasizer Nano ZS 90 (Malvern Instruments, Worcestershire, UK). All sample measurements were performed at a scattering angle of 173 °C and a temperature of 25 °C. Samples for each NP batch were measured five times and the average value ± the standard deviation reported. Size distribution peaks by intensity were also recorded for each measurement.
NP morphology was observed by transmission electron microscopy (TEM). Aqueously dispersed NPs were added to a formvar grid stabilised with silicon monoxide. Following evaporation of the dispersant, sample grids were viewed using a Hitachi H-7650 electron microscope (Hitachi High-Technologies, Leixlip, Ireland).
Mohan L.J., McDonald L., Daly J.S, & Ramtoola Z. (2020). Optimising PLGA-PEG Nanoparticle Size and Distribution for Enhanced Drug Targeting to the Inflamed Intestinal Barrier. Pharmaceutics, 12(11), 1114.
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3

Ultrastructural Analysis of Sciatic Nerve

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Mice under deep anesthesia were transcardially perfused with PBS heparin and sciatic nerves were then fixed in situ with 2.5% glutaraldehyde + 2% paraformaldehyde in 0.1 M PBS, pH 7.4. Tissues were then embedded in epon, and semi-thin sections (500 nm) were stained with toluidine blue using standard procedures. Ultrathin sections were mounted on copper grids coated with Formvar membrane and contrasted with uranyl acetate/lead citrate. Specimens were examined using a Hitachi H-7650 electron microscope (Hitachi High-Tech) operating at 80 kV.
Nuvolone M., Hermann M., Sorce S., Russo G., Tiberi C., Schwarz P., Minikel E., Sanoudou D., Pelczar P, & Aguzzi A. (2016). Strictly co-isogenic C57BL/6J-Prnp−/− mice: A rigorous resource for prion science. The Journal of Experimental Medicine, 213(3), 313-327.
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4

Ultrastructure Analysis of Brachial Plexus

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Dissected brachial plexus was placed in 4% PFA overnight then rinsed and transferred to 10 mL pH 7.2 containing 2.5% glutaraldehyde-0.1 M imidazole buffer. Tissues were then immersed in 2 mL 2% osmium tetroxide in imidazole buffer for 4 hr and washed in distilled water followed by dehydration in a graded series of ethanol (50%, 80%, absolute). Sequentially, tissue sections were immersed in a mixture of 1:1 absolute ethanol and propylene oxide and then in propylene oxide in sealed vials on a rotating mixer for 15 min. Finally, tissue was infiltrated with a 1:1 mixture of propylene oxide and epoxy resin overnight in open vials then embedded with epoxy resin in molds at 60°C for 48 hr. Embedded tissue was first ‘thick’ sectioned at 0.25 micrometers with glass knives and stained with epoxy tissue stain (Electron Microscopy Sciences, Hatfield, PA). Selected areas were then thin sectioned at 70 nm with diamond knives and mounted on Formvar-Carbon coated grids and stained with 2% uranyl acetate and lead citrate solution. Digital images were collected with a mid-mount XR611 camera (AMT, Inc., Woburn, MA) in a model H-7650 electron microscope (Hitachi High-Technologies, Dallas, TX).
Vidal-Martinez G., Najera K., Miranda J.D., Gil-Tommee C., Yang B., Vargas-Medrano J., Diaz-Pacheco V, & Perez R.G. (2019). FTY720 Improves Behavior, Increases Brain Derived Neurotrophic Factor and Reduces α-Synuclein Pathology in Parkinsonian GM2+/− Mice. Neuroscience, 411, 1-10.
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5

Visualizing Cellular Morphology and Mitochondria

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To visualize cellular morphological changes, all cells cultured in their corresponding pHe medium were collected and plated in 35-mm diameter μ-dishes (Ibidi, Martinsried, Germany). A minimum of 10 randomly chosen microscopic fields (~ 70 cells per field) were imaged using an AF6000 LX microscope (Leica Microsystems, Singapore) equipped with Zyla sCMOS camera (Andor by Oxford Instruments, Belfast, UK) and objective lens (HCX PL Fluotar L 20x/0.40 CORR PH1) with 1.8x digital zoom. To observe mitochondrial morphology and cristae ultrastructure, the TEM (transmission electron microscopy) images of each cell group were taken on an H-7650 electron microscope (Hitachi High-Technologies, Tokyo, Japan) equipped with a CCD (charge-coupled device) camera (Advanced Microscopy Techniques, Danvers, MA, USA). The density of cristae was calculated based on 45 randomly selected mitochondria per cell group with ImageJ software (https://imagej.nih.gov/ij).
Wu T.C., Liao C.Y., Lu W.C., Chang C.R., Tsai F.Y., Jiang S.S., Chen T.H., Lin K.M., Chen L.T, & Chang W.S. (2022). Identification of distinct slow mode of reversible adaptation of pancreatic ductal adenocarcinoma to the prolonged acidic pH microenvironment. Journal of Experimental & Clinical Cancer Research : CR, 41, 137.
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6

Ultrastructural Analysis of Leukoplakia Tissue

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The small samples of leukoplakia tissues were immediately fixed with 2% paraformaldehyde and 2.5% glutaraldehyde in 0.1 M phosphate buffer (PB) (pH 7.4) at room temperature for 2 h. Following washing with 0.1 M PB twice for 10 min at 4°C, the specimens were post-fixed in 1% osmium tetroxide in 0.1 M PB at 4°C for 2 h. Dehydration of the specimens through a graded series of ethanol solutions was followed by substitution with N-butyl glycidyl ether (QY-1) (Nisshin EM, Tokyo, Japan) twice for 15 min and then with QY-1 and epoxy resin (Epon 812) (TAAB Laboratories, Aldermaston, Berks, UK) mixed 1:1 overnight at room temperature and final infiltration with Epon 812 for 6 h at room temperature. Polymerization was performed by incubation at 60°C for 3 days.
The resin-embedded samples were cut into ultrathin sections (70–80 nm thickness), and the sections were collected on copper grids (Nisshin EM). The sections were stained with 1% uranyl acetate in 0.1 M PB for 30 min and 1% lead nitrate in 0.1 M PB for 5 min, and they were then observed under an H-7650 electron microscope (Hitachi High-Technologies, Tokyo, Japan).
Mizuki H., Abe R, & Mikami T. (2017). Ultrastructural Changes during the Life Cycle of Mycoplasma salivarium in Oral Biopsies from Patients with Oral Leukoplakia. Frontiers in Cellular and Infection Microbiology, 7, 403.
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7

Electron Microscopic Examination of Bacterial Cells

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Samples were collected from the fermentation at the late-lag (T1), mid-exponential (T2), late-exponential (T3), and stationary (T4) phases. At the indicated time points, the cells were harvested through centrifugation at 8000 r/min for 15 min at 4°C. Cells were diluted in buffer as required after washing twice with PBS buffer (50 mmol l–1, pH 6.5). The electron microscopic investigations were carried out using a HITACHI H-7650 electron microscope (Hitachi High-Technologies, Tokyo, Japan). The formvar-coated copper grids were immersed in the bacterial suspension for 10 min; then stained with 2% phosphotungstic acid (PTA) for 5 s after the removal of excess liquid and air drying; the grid was air- dried again before examination.
Qiao Y., Liu G., Lv X., Fan X., Zhang Y., Meng L., Ai M, & Feng Z. (2020). Metabolic Pathway Profiling in Intracellular and Extracellular Environments of Streptococcus thermophilus During pH-Controlled Batch Fermentations. Frontiers in Microbiology, 10, 3144.
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8

Ultrastructural Analysis of HL-60 Cells

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HL-60 cells treated with or without benfotiamine were fixed by immersion in 2% paraformaldehyde plus 2.5% glutaraldehyde in 0.1 M phosphate buffer (pH 7.4) for 1 hour at 4°C. After washing in 0.1 M phosphate buffer at 4°C, the cells were postfixed in 1% osmium tetroxide for 1 hour at 4°C. They were then washed repeatedly in distilled water, stained with 1% uranyl acetate for 30 min, dehydrated through graded ethanol series and propylene oxide, and embedded in Glicidether (Selva Feinbiochemica, Heidelberg, Germany). Ultrathin sections were cut and mounted onto copper grids, stained with 1% uranyl acetate for 10 min followed by Reynolds lead citrate for 5 min, and then observed on an H-7650 electron microscope (Hitachi High-Technologies, Tokyo, Japan).
Sugimori N., Espinoza J.L., Trung L.Q., Takami A., Kondo Y., An D.T., Sasaki M., Wakayama T, & Nakao S. (2015). Paraptosis Cell Death Induction by the Thiamine Analog Benfotiamine in Leukemia Cells. PLoS ONE, 10(4), e0120709.
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9

Ultrastructural Analysis of Virus Particles

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Transmission electron microscopy was performed to observe the virion in the cultural supernatant. Virus samples were recovered from the TC supernatant in Vero E6 cells after centrifugation at 32,000 rpm at 4 °C for 3 h through a 20% sucrose cushion. A suspension fixed with 0.25% glutaraldehyde was adsorbed to collodion-carbon-coated copper grids (400 mesh; Nisshin EM Co., Tokyo, Japan) and negatively stained with 2% phosphotungstic acid (pH 5.8). The sample was observed with an H-7650 electron microscope (Hitachi High-Technologies, Tokyo, Japan) at 80 kV.
Ogawa H., Kajihara M., Nao N., Shigeno A., Fujikura D., Hang’ombe B.M., Mweene A.S., Mutemwa A., Squarre D., Yamada M., Higashi H., Sawa H, & Takada A. (2017). Characterization of a Novel Bat Adenovirus Isolated from Straw-Colored Fruit Bat (Eidolon helvum). Viruses, 9(12), 371.
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10

Electron Microscopy Sample Preparation

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Samples were fixed with 2.5% glutaraldehyde solution, buffered at pH 7.4 with 0.1 M Millonig’s phosphate at 4 ºC for 2 h, postfixed in 1% osmium tetroxide solution at 4 ºC for 1 h, dehydrated in graded concentrations of ethanol, and embedded in epoxy resin (Quetol 812; Nissin EM Co. Ltd., Tokyo, Japan). Ultrathin sections (80 nm) were cut on a Reichert Ultracut E ultramicrotome (Reichert, Nu loch, Germany), stained with uranyl acetate and lead citrate, and examined with an H-7650 electron microscope (Hitachi High-Technologies, Tokyo, Japan) at 80 kV.
Zhu W., Wang X., Zhou Y, & Wang H. (2014). C2-Ceramide Induces Cell Death and Protective Autophagy in Head and Neck Squamous Cell Carcinoma Cells. International Journal of Molecular Sciences, 15(2), 3336-3355.
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