Coulter stks

The toxic outcome of subacute or subchronic oral administration of MEMM was evaluated using the samples collected as described above. The whole blood in the EDTA-containing vacutainers was processed within 24 h and then subjected to the haematological analysis using the automated analyser (Coulter STKS, Beckman) to yield information on several haematological parameters (i.e., total red blood cell (RBC) count, haemoglobin (Hb), mean corpuscular haemoglobin concentration (MCHC), mean corpuscular volume (MCV), packed cell volume (PCV), total white blood cell (WBC) count, neutrophils, monocytes, lymphocytes, and eosinophils).
The serum, which was earlier kept at −20°C, was thawed at 25°C and treated within two days following the blood collection. Biochemical analysis was performed using an automated biochemical analyser (Hitachi 902, Japan) for several biochemical parameters (i.e., alanine aminotransferase (ALT), alkaline phosphatase (ALP), aspartate aminotransferase (AST), creatinine (Crea), urea, and total bilirubin (TBil)). The control group mean values were used as the baseline for comparison with treatment groups.

Peripheral blood samples (2 ml, ethylenediaminetetraacetic acid [EDTA]) were drawn from each patient (inlet line) prior to and two hours after completion of leukapheresis. Leukapheresis product samples with EDTA (2 ml) were obtained for laboratory analysis. Pre-leukapheresis and post-leukapheresis complete blood counts (CBC) and collected product were performed to determine the blood routine examination with an automated blood cell analyser Coulter STKS (Beckman Coulter, USA). Harvest product was further analysed for volume, the numbers of collected total nucleated cells.
The collection efficiencies (CE_WBC) were calculated based on patient’s blood volume by the following formula^{42 (link),43 (link)}: $$\begin{array}{c}\mathbf{C}\mathbf{E}(\mathbf{r}\mathbf{a}\mathbf{t}\mathbf{e}\mathbf{o}\mathbf{f}\mathbf{l}\mathbf{e}\mathbf{u}\mathbf{k}\mathbf{o}\mathbf{c}\mathbf{y}\mathbf{t}\mathbf{e}\mathbf{d}\mathbf{e}\mathbf{p}\mathbf{l}\mathbf{e}\mathbf{t}\mathbf{i}\mathbf{o}\mathbf{n})\\ =\frac{\mathrm{W}\mathrm{B}\mathrm{C}\mathrm{c}\mathrm{o}\mathrm{u}\mathrm{n}\mathrm{t}\mathrm{s}\mathrm{i}\mathrm{n}\mathrm{c}\mathrm{o}\mathrm{l}\mathrm{l}\mathrm{e}\mathrm{c}\mathrm{t}\mathrm{e}\mathrm{d}\mathrm{p}\mathrm{r}\mathrm{o}\mathrm{d}\mathrm{u}\mathrm{c}\mathrm{t}\times \mathrm{v}\mathrm{o}\mathrm{l}\mathrm{u}\mathrm{m}\mathrm{e}\mathrm{o}\mathrm{f}\mathrm{c}\mathrm{o}\mathrm{l}\mathrm{l}\mathrm{e}\mathrm{c}\mathrm{t}\mathrm{e}\mathrm{d}\mathrm{p}\mathrm{r}\mathrm{o}\mathrm{d}\mathrm{u}\mathrm{c}\mathrm{t}(\mathrm{m}\mathrm{l})}{\mathrm{W}\mathrm{B}\mathrm{C}\mathrm{c}\mathrm{o}\mathrm{u}\mathrm{n}\mathrm{t}\mathrm{s}\mathrm{p}\mathrm{r}\mathrm{e}-\mathrm{a}\mathrm{p}\mathrm{h}\mathrm{e}\mathrm{r}\mathrm{e}\mathrm{s}\mathrm{i}\mathrm{s}\times \mathrm{t}\mathrm{o}\mathrm{t}\mathrm{a}\mathrm{l}\mathrm{b}\mathrm{l}\mathrm{o}\mathrm{o}\mathrm{d}\mathrm{v}\mathrm{o}\mathrm{l}\mathrm{u}\mathrm{m}\mathrm{e}(\mathrm{m}\mathrm{l})}\times 100\mathrm{\%}\end{array}$$
In addition, the RBC and PLT contamination were also evaluated. $$\begin{array}{l}\mathbf{blood}\mathbf{cell}\mathbf{reduction}\\ =\mathrm{blood}\mathrm{cell}\mathrm{counts}\mathrm{before}\mathrm{leukocytapheresis}-\mathrm{blood}\mathrm{cell}\mathrm{counts}\mathrm{after}\mathrm{leukocytapheresis}\mathrm{.}\end{array}$$

Red blood cell distribution width measurement was conducted with peripheral venous blood using an automated hematology analyzer Coulter STKS (Beckman Coulter). The Coulter STKS presented good performance with low false‐negative rate (1.8%), high sensitivity (96.3%), and specificity (83.3%) (Verheul, Spitters, & Bergmans, 1993). Other collected laboratory parameters included white blood cell count, red blood cell count, hemoglobin, platelet count, hematocrit, mean corpuscular volume, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration, fasting blood glucose, blood urea nitrogen, creatinine, uric acid, total cholesterol, triglyceride, high‐density lipoprotein cholesterol, and low‐density lipoprotein cholesterol.