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4 protocols using fuchsine

1

Mango Seed Kernel Sorption of RIFM and TIGC

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All drugs including, RIFM, TIGC, amantadine, acetaminophen, sofosbuvir, acyclovir, and ivermectin were products of Biosynth® Carbosynth Ltd. (UK). Cerium (III) nitrate hexahydrate was product of Fluka–Garantie (Switzerland). Ammonia solution (26%) was product of Honeywell Riedel-deHaën AG (Germany). Oleylamine, Ammonia solution (25%), rose bengal, fuchsine, methylene blue, ibuprofen sodium, and rest of chemicals were commodities of Sigma–Aldrich (USA). Deionized water (DW) (Millipore-Q water supply, 18.2 MΩ cm) was used thru this study. The ripened mango fruits were obtained locally, and the seeds were separated from the fruit. The hard seed coat was then removed, and the kernel was taken out (MSK). Stock solutions (100 ppm) of RIFM and TIGC and their following dilutions were prepared in DW and used for the following batch sorption studies. An aqueous solution of either HCl or NaOH (0.1 M) was utilized to fine-tune the pH to the requested value.
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2

Characterization of Membrane Filters

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Remarks on the intact PTFE membrane, porous PTFE membrane, filter paper, nitrocellulose filter (NCF), polycarbonate membrane (PCM), and JHWP04700 are listed in Table 1. The contents of a conventional material transfer agreement restricted the disclosure of information on iPTFE and pPTFE.
Reagents: amide black 10 B (AB), bromothymol blue (BTB), Coomassie brilliant blue G250 (CBB), fluorescein (FL), fuchsine (FC), LDC, malachite green oxalate (MG), methylene blue (MB), methyl orange (MO), rhodamine 6G (R6G), Sudan III (SD) were purchased from Sigma-Aldrich (St. Louis, MO, USA), and Wako Pure Chemical Industries (Osaka, Japan). All other reagents were of the highest commercially available grade.
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3

Chemical Reagents for Biological Assays

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Sodium hydroxide, ammonium, sodium phosphate, sodium carbonate anhydrous, sodium hypochlorite, TEA, and DEA were from Merck (Germany). Fuchsine, Giemsa, thionine, and CoCl2 were purchased from Sigma-Aldrich (USA).
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4

Phenotypic Characterization of Rev1::gfp Clones

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Three clones of Rev1::gfp showing the desired genetic characteristics were selected for further phenotypic characterization. Colonial size, crystal violet‐oxalate exclusion, catalase, oxidase, urease and acriflavine tests (all products from Sigma Aldrich, Spain), sensitivity to Tb, Wb, Iz and R/C phages, agglutination with anti‐A and anti‐M monospecific sera, both CO2‐ and serum‐ dependence, susceptibility to both dyes (i.e., thionine blue 10, 20 and 40 μg/ml, fuchsine 10 and 20 μg/ml, and safranin 100 μg/ml; Sigma) and antibiotics (i.e., penicillin and streptomycin) markers were assessed by standard protocols (Alton, Jones, Angus, & Verger, 1988).
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