Mueller hinton agar (mha)

All chemicals and regents used for this study were analytical grade and used without further purification. 1,10-phenanthroline monohydrate (BDH chemical Ltd., Poole, England), copper (II) acetate monohydrate [Cu(CO₂CH₃)₂·H₂O], triethylamine, NaHCO₃, NaOH, and Mueller-Hinton agar were purchased from Loba Chemie PVT Ltd., Addis Ababa. Metformin hydrogen chloride (Met.HCl) and ciprofloxacin hydrogen chloride (Cip.HCl) were obtained from Cadila Pharmaceuticals PLC, Ethiopia. Methanol, ethanol, HCl, DMSO, ethyl acetate and dichloromethane (DCM) were purchased from Alpha Chemika, Addis Ababa, Ethiopia. Thin Layer Chromatography (MERCK Silica gel 60 F254) together with UV Cabinet (UV-Vis lamp at 254 and 365 nm) were used to monitor the progress of the chemical reactions. The melting points of the complexes were determined using capillary tubes (Thiele tube). UV-Vis spectrophotometer (SM-1600 Spectrophotometer), FTIR (Perkin-Elmer BX spectrometer, Shimadzu Corporation, Japan), TGA/DTC (DTG-60H SHIMADZU thermal analyzer) and High resolution mass spectra (Waters-LCT-Premier mass spectrometer) were used to characterize the synthesized metal complexes.

Ampicillin, ciprofloxacin, methanol (RFCL limited, New Delhi, India), nutrient broth (India), blood agar, MacConkey agar, Mueller Hinton Agar, biochemical testing, DMSO (dimethyl sulphoxide- Loba Chemie Pvt.Ltd. Mumbai, India), phytochemical screening reagents, ketamine (ROTEXMEDICA, Germany), simple ointment base and nitrofurazone ointment USP 0.2% (Galentic pharma, India Pvt. Ltd.) were purchased and collected from the respective sources.