Female balb c nude mice

Manufactured by Charles River Laboratories

Sourced in China, Japan, United States, Italy, United Kingdom, France, Germany

The female BALB/c nude mice are an immunodeficient mouse model. They lack a functional thymus and are unable to produce mature T cells, resulting in a severely compromised immune system.

Automatically generated - may contain errors

Lab products found in correlation

Matrigel, by BD (12 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (6 mentions) Penicillin, by Thermo Fisher Scientific (5 mentions) Streptomycin, by Thermo Fisher Scientific (5 mentions) Matrigel, by Corning (5 mentions) Fetal calf serum (fcs), by Thermo Fisher Scientific (4 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (4 mentions) Micro4 micro syringe pump controller, by World Precision Instruments (3 mentions) Balb c mice, by Charles River Laboratories (3 mentions) Female kunming mice, by Charles River Laboratories (2 mentions) Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (2 mentions) Small animal multi spectral living imaging system, by Berthold Technologies (2 mentions) Ivis spectrum in vivo optical imaging system, by PerkinElmer (2 mentions) Nod scid mice, by Charles River Laboratories (2 mentions) Nightowl 2 lb983, by Berthold Technologies (2 mentions) Matrigel basement membrane matrix, by BD (2 mentions) Living image software, by PerkinElmer (2 mentions) Pentobarbital sodium, by Merck Group (2 mentions) Ab16667, by Abcam (2 mentions) Female balb c mice, by Charles River Laboratories (2 mentions)

309 protocols using female balb c nude mice

Breast Cancer Xenograft Model in Nude Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols

All the animal experiments were performed according to the NIH guidelines for the care and use of laboratory animals of Peking University Animal Study Committee’s requirements and were according to the protocol approved by the Institutional Animal Care. Mice were maintained under specific pathogen-free conditions and all the efforts were made to minimize animal suffering.
Female BALB/c nude mice at 6 weeks of age (initially weighing almost 16 g) were purchased from Vital River Laboratory Animal Technology Co. Ltd. Four subtypes of breast cancer cells were trypsinized using Trypsin-EDTA (0.25%) containing phenol red, washed once with PBS, re-suspended in culture medium at 4 × 10⁶ cells per 200 µl, and injected in triplicate into mammary fat pads of Female BALB/c nude mice. Mice were monitored daily for 6 weeks. Tumor size was measured every two days with calipers. The tumor volume was determined by the following formula:

V = L \times \frac{W^{2}}{2}

(Car lsson et al., 1983). Mice were euthanized. The primary tumor, along with the heart, liver, spleen, lungs, and kidneys were collected from an individual mouse after euthanization at the end of the study (after 6 weeks), and fixed in 4% paraformaldehyde (PFA) (Sigma) at 4 °C and embedded in paraffin for further evaluation.

Li W., Ma H., Zhang J., Zhu L., Wang C, & Yang Y. (2017). Unraveling the roles of CD44/CD24 and ALDH1 as cancer stem cell markers in tumorigenesis and metastasis. Scientific Reports, 7, 13856.

+ Open protocol

+ Expand

Xenograft Tumor Formation and Immunohistochemistry

Check if the same lab product or an alternative is used in the 5 most similar protocols

Animal studies were carried out according to the Guideline for Animal Experiments in National Cancer Center and Osaka university, which meet the ethical standards required by the law and the guidelines for use of experimental animals in Japan, and approved by the Committee for Ethics in Animal Experimentation of the National Cancer Center (T15-004) and Osaka university (30-084-003). A 100 mL volume of 1 Â 10 7 cells (ELF3-knockout or wild-type cells) in a 1:1 mixture of Matrigel (354234, BD Biosciences) was subcutaneously injected into 6 weeks old female BALB/c nude mice (Charles River Laboratories). Three months after inoculation, tissue sections were fixed with formalin and embedded in paraffin. Five mice used in each group. A 50 mL volume of 1 Â 10 6 HBDEC2 ELF3À/À EMR ELF3-ERT2 cells in a 1:1 mixture of Matrigel (BD Biosciences) was subcutaneously injected into 7-week-old female BALB/c nude mice (Charles River Laboratories). Mice were housed with drinking water supplemented with or without tamoxifen (AstraZeneca; 0.1 mg/mL in 5% sucrose). Twenty-one days after inoculation, tissue sections were fixed with formalin and embedded in paraffin. Three mice were used in each group. IHC was performed using rabbit anti-vimentin primary antibody (1:200, Abcam) with EnVisionþ System-HRP labeled polymer anti-Rabbit (K4002, Dako-Agilent).

Suzuki M., Saito-Adachi M., Arai Y., Fujiwara Y., Takai E., Shibata S., Seki M., Rokutan H., Maeda D., Horie M., Suzuki Y., Shibata T., Kiyono T, & Yachida S. (2021). E74-Like Factor 3 Is a Key Regulator of Epithelial Integrity and Immune Response Genes in Biliary Tract Cancer. Cancer research, 81(2).

+ Open protocol

+ Expand

Xenograft Tumor Establishment in Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols

BALB/c‐nude female mice were purchased from Beijing Vital River Laboratory Animal Technology Co., Ltd. After a week of acclimatization, the transfected SiHa and Me180 cells were digested and obtained as a 5 × 10⁶/mL single cell suspension and injected into the left armpit of BALB/c‐nude female mice with a 1 mL syringe following disinfection. After subcutaneous solid tumors were formed in the mice, the animals were sacrificed and the tumor tissues were excised and weighed.

Wang J., Wang S., Wang J., Huang J., Lu H., Pan B., Pan H., Song Y., Deng Q., Jin X, & Shi G. (2023). Comprehensive analysis of clinical prognosis and biological significance of CNIH4 in cervical cancer. Cancer Medicine, 12(24), 22381-22394.

+ Open protocol

+ Expand

Xenograft Tumor Growth Assay in Nude Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols

Female BALB/c-nude mice (4–6 weeks old) were purchased from Vital River Laboratory Animal Technology (Beijing, China). Mice were fed in specific pathogen free animal room at 22°C-25°C and 40–50% humidity with free access to water and food. The transfected C33A cells (5×10⁵) were subcutaneously injected into mice. The tumor volume was measured using vernier caliper every 5 days. After the injection for 35 days, the tumor weight was measured on an analytical balance. Animal experiment was approved by the Animal Care and Use Committee of the Affiliated Hospital of Qingdao University (Examination and Approval Number of Ethics Committee: 2016-Gynaecology-021103).

Meng M., Sang L, & Wang X. (2019). S100 Calcium Binding Protein A11 (S100A11) Promotes The Proliferation, Migration And Invasion Of Cervical Cancer Cells, And Activates Wnt/β-Catenin Signaling. OncoTargets and therapy, 12, 8675-8685.

+ Open protocol

+ Expand

Tumor Growth Inhibition in BALB/c Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols

Female BALB/c nude mice (4–5 weeks old) were purchased from the Vital River Laboratory Animal Technology Company (Beijing, China). All mice were randomly assigned to two groups. MKN-45 cells (5 ×10⁵) with stable knockdown of USP13 or control were harvested and re-suspended in 100 μL PBS. Then the cells were subcutaneously injected into the mice. Tumor size was monitored by measuring the length (L) and width (W) of the tumor every 2 days with a caliper, and the tumor volume (V) was calculated with the formula V = 1/2 × L × W². Twenty-six days after injection, the mice were euthanized, and the tumors were weighed. All mice experiments were approved by the ethics committee of the School of Basic Medical Sciences, Shandong University and performed in accordance with the guidance of animal experiments in the Laboratory Animal Center of Shandong University.

Ma C., Wang D., Tian Z., Gao W., Zang Y., Qian L., Xu X., Jia J, & Liu Z. (2023). USP13 deubiquitinates and stabilizes cyclin D1 to promote gastric cancer cell cycle progression and cell proliferation. Oncogene, 42(29), 2249-2262.

+ Open protocol

+ Expand

In Vivo Evaluation of miR-124 Suppressing Tumor Growth

Check if the same lab product or an alternative is used in the 5 most similar protocols

Female BalB/C nude mice were purchased from the Beijing Vital River Laboratory Animal Technology Co., Ltd (Beijing, China) aged 4-6 weeks. Animals were subcutaneously injected HCCLM3-LV-miR-CON or HCCLM3-LV-miR-124 cells containing 1×10⁶ into the dorsal flanks of mouse to detect the vivo tumorigenic capacity. The tumor growth was assessed with electronic calipers by recording tumor length and width for 4 weeks. Subsequently, the animals were sacrificed and tumor mass was collected to weigh and photograph. Animal experiments were done in accordance with protocols approved by the Animal Care and Use Committee of Qingdao University.

Cao J., Qiu J., Wang X., Lu Z., Wang D., Feng H., Li X., Liu Q., Pan H., Han X., Wei J., Liu S, & Wang L. (2018). Identification of microRNA-124 in regulation of Hepatocellular carcinoma through BIRC3 and the NF-κB pathway. Journal of Cancer, 9(17), 3006-3015.

+ Open protocol

+ Expand

Tumor Xenograft Mouse Model for VLP Therapy

Check if the same lab product or an alternative is used in the 5 most similar protocols

Female BALB/c nude mice (3–4 weeks old, specific pathogen-free/viral antibody-free, SPF/VAF) were purchased from Vital River (Beijing, China), and fed a standard diet, under the conditions of SPF isolation in the Institute of Laboratory Animal Science (Beijing, China). All animals used in this experiment were approved by the Ethics Committee of the National Center for Clinical Laboratories. Hep3B cells (1 × 10⁷), combined with BD Matrigel Basement Membrane Matrix (BD, Franklin Lakes, NJ), were subcutaneously injected into the posterior flank of each mouse. When the tumor reached approximately 0.5 cm³, observed with naked eye, the mice were ready for VLP administration, after being randomly grouped. Four groups were designed in this experiment: (i) treated with 2MS-TAT-miRNC VLPs (5 mice), (ii) treated with MS-miRNC VLPs crosslinked with TAT (5 mice), (iii) treated with 2MS-TAT-miR122 VLPs (7 mice), and (iv) treated with MS-miR122 VLPs crosslinked with TAT (7 mice). VLPs (100 ng per administration) were administrated twice a week via tail vein for 3 weeks. Tumor volumes were calculated using the equation,

V = 0.5 \times a \times b^{2}

where V is volume, a is longitudinal diameter and b is latitudinal diameter.

Wang G., Jia T., Xu X., Chang L., Zhang R., Fu Y., Li Y., Yang X., Zhang K., Lin G., Han Y, & Li J. (2016). Novel miR-122 delivery system based on MS2 virus like particle surface displaying cell-penetrating peptide TAT for hepatocellular carcinoma. Oncotarget, 7(37), 59402-59416.

+ Open protocol

+ Expand

Xenograft Tumor Model in BALB/C Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols

Female BALB/C nude mice of 6–8 weeks old (Vital River Laboratories, VRL, Beijing, China) were housed within a dedicated SPF facility at Laboratory Animal Center of PUMCH. Stable Hela cells with over-expressing miR-497 or blank controls were harvested, and injected into right-side flank of mice subcutaneously at a concentration of 4 × 10⁷ cells/ml in PBS (100 μl/mouse).The mice were monitored after injection and tumors were measured every two days with a vernier caliper. All mice were killed on day 30 after injection and subcutaneous tumors were surgically excised, weighed, photographed, sectioned, and stained with haematoxylin-eosin. Animal experiments were approved by PUMCH animal ethics committee and the methods were carried out in accordance with the approved guidelines.

Zhang Y., Zhang D., Wang F., Xu D., Guo Y, & Cui W. (2015). Serum miRNAs panel (miR-16-2*, miR-195, miR-2861, miR-497) as novel non-invasive biomarkers for detection of cervical cancer. Scientific Reports, 5, 17942.

+ Open protocol

+ Expand

Breast Cancer Xenograft Model in BALB/c Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols

Female BALB/c nude mice (4–6 weeks old) were purchased from Vital River Laboratory Animal Technology Co. Ltd. (China). Animals received care in accordance with the Guidance Suggestions for the Care and Use of Laboratory Animals. All the animals received care complied with the guidelines in the Guide for the Care and Use of Laboratory Animals, and the procedures of animal experiments were approved by the Institutional Ethical Committee of Animal Experimentation of Shenzhen Institutes of Advanced Technology (Chinese Academy of Sciences). The methods were carried out strictly in accordance with governmental and international guidelines on animal experimentation. All efforts were made to minimize the usage amount of animals and the suffering during experiments according to the request of Biosafety and Animal Ethics. MCF-7 cells (1 × 10⁶) were administered by subcutaneous injection into the flank region of the mice. Tumour volume was calculated as (tumour length) × (tumour width)²/2.

Luo Z., Zheng M., Zhao P., Chen Z., Siu F., Gong P., Gao G., Sheng Z., Zheng C., Ma Y, & Cai L. (2016). Self-Monitoring Artificial Red Cells with Sufficient Oxygen Supply for Enhanced Photodynamic Therapy. Scientific Reports, 6, 23393.

+ Open protocol

+ Expand

Investigating Circular RNA in Lung Cancer

Check if the same lab product or an alternative is used in the 5 most similar protocols

Animal experiments were approved by the Affiliated Hospital of Jiangnan University. Female BALB/c nude mice (5–6 weeks old, n = 24) were purchased from Vital River Laboratory Animal Technology Co., Ltd. A549 cells was stable transfected with sh‐circ_0010235 or sh‐NC. Subsequently, the transfected cells were injected into BALB/C nude mice. The grouping information was as follow: sh‐NC + PBS, sh‐circ_0010235 + PBS, sh‐NC + 2‐MeOE2, sh‐circ_0010235 + 2‐MeOE2. After 8 days, mice were intraperitoneally injected with 40 mg/kg of 2‐MeOE2 every 3 days. The tumor volume was counted every 3 days according to the formula: volume = 0.5 × length×width². After 23 days, all mice were killed, tumor tissues were removed and weighed, and tumor masses were collected for further determination.

Zhang Y., Mi Y, & He C. (2023). 2‐methoxyestradiol restrains non‐small cell lung cancer tumorigenesis through regulating circ_0010235/miR‐34a‐5p/NFAT5 axis. Thoracic Cancer, 14(22), 2105-2115.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!