Cell morphological features like size and shape, and cell division were observed under eld emission scanning electron microscopy (FESEM) or Transmission electron microscopy (TEM). For FESEM, one ml of log-phase culture was centrifuged at 7000xg for 10 min at 4°C. The resulted cell pellet was washed by re-suspending in sterile MilliQ and centrifuged at 7000 g for 10 min at 04°C. The pellet or cells were xed in 2.5% glutaraldehyde solution and kept for six hours incubation at 4°C. Cells were dehydrated sequentially with an increased ethanol concentration from 10-100% (v/v) (10% interval). At last, the cells were resuspended in 100% ethanol. 10µl of samples were kept on a small size glass slide, which was placed on the stab with adhesive tape (Kumar et al. 2021 ). Finally, stabs were kept for gold sputtering for six minutes and then cell morphology and division were viewed under the FESEM (Philips XL3O) facility of school of Physics, UOH. For TEM, ultrathin sectioning of the log phase cells was outsourced to RUSKA Diagnostic, Hyderabad. The sections of the cells were mounted on copper grids and observed under TEM (H-7500 Hitachi) facility of CCMB, Hyderabad.
H 7500
Manufactured by Hitachi
Sourced in Japan, United States, Germany, France
The H-7500 is a transmission electron microscope (TEM) manufactured by Hitachi. It is designed for high-resolution imaging and analysis of materials at the nanoscale level. The core function of the H-7500 is to provide researchers and scientists with a powerful tool for studying the structure, composition, and properties of a wide range of samples, including biological, materials, and semiconductor specimens.
Automatically generated - may contain errors
Lab products found in correlation
Em uc6, by Leica (11 mentions)
Zetasizer nano z, by Malvern Panalytical (9 mentions)
Em uc7, by Leica (7 mentions)
V 570, by Jasco (5 mentions)
Zetasizer nano zs90, by Malvern Panalytical (5 mentions)
Smartlab, by Rigaku (4 mentions)
Jem 2100f, by JEOL (4 mentions)
Epon 812, by Merck Group (4 mentions)
Exoquick exosome precipitation solution, by System Biosciences (4 mentions)
Em uc6 ultramicrotome, by Leica (4 mentions)
Ckx41, by Olympus (3 mentions)
Pkh67 membrane dye, by Merck Group (3 mentions)
Glutaraldehyde, by Electron Microscopy Sciences (3 mentions)
S 4800, by Hitachi (3 mentions)
Nano zs90, by Malvern Panalytical (3 mentions)
Su8220, by Hitachi (3 mentions)
Spurr s resin, by Merck Group (3 mentions)
Bca protein assay kit, by Beyotime (3 mentions)
Delsa nano ctm, by Beckman Coulter (3 mentions)
Uc7 ultramicrotome, by Leica (3 mentions)
619 protocols using h 7500
1
Microscopic Analysis of Cell Morphology
2
Microscopic Analysis of Cell Morphology
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Cell morphological features like size and shape, and cell division were observed under eld emission scanning electron microscopy (FESEM) or Transmission electron microscopy (TEM). For FESEM, one ml of log-phase culture was centrifuged at 7000xg for 10 min at 4°C. The resulted cell pellet was washed by re-suspending in sterile MilliQ and centrifuged at 7000 g for 10 min at 04°C. The pellet or cells were xed in 2.5% glutaraldehyde solution and kept for six hours incubation at 4°C. Cells were dehydrated sequentially with an increased ethanol concentration from 10-100% (v/v) (10% interval). At last, the cells were resuspended in 100% ethanol. 10µl of samples were kept on a small size glass slide, which was placed on the stab with adhesive tape (Kumar et al. 2021 ). Finally, stabs were kept for gold sputtering for six minutes and then cell morphology and division were viewed under the FESEM (Philips XL3O) facility of school of Physics, UOH. For TEM, ultrathin sectioning of the log phase cells was outsourced to RUSKA Diagnostic, Hyderabad. The sections of the cells were mounted on copper grids and observed under TEM (H-7500 Hitachi) facility of CCMB, Hyderabad.
3
Electron Microscopy of Infected FBM Cells
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For electron microscopy, infected FBM cells were detached from the petri dish using a
rubber policeman. The cells were fixed with 2.5% glutaraldehyde in PBS, pelleted by
centrifugation, post fixed with osmium tetroxide (1% in 0.1 M phosphate buffer, pH 7.4),
dehydrated in graded ethanol, and embedded in LUVEAK-812 resin. Ultrathin sections were
stained with uranyl acetate and lead citrate, and observed using a transmission electron
microscope (H-7500, Hitachi High-Technologies Corp., Tokyo, Japan).
rubber policeman. The cells were fixed with 2.5% glutaraldehyde in PBS, pelleted by
centrifugation, post fixed with osmium tetroxide (1% in 0.1 M phosphate buffer, pH 7.4),
dehydrated in graded ethanol, and embedded in LUVEAK-812 resin. Ultrathin sections were
stained with uranyl acetate and lead citrate, and observed using a transmission electron
microscope (H-7500, Hitachi High-Technologies Corp., Tokyo, Japan).
4
Ultrastructural Analysis of hiPSC-Derived Retinal Organoids
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hiPSC-derived retinal organoids were submitted to the Electron Microscope Laboratory at Tzu Chi University for ultrastructural analysis using their standard protocol. Briefly, the organoids were fixed in a cold EM fixative (2.5% glutaraldehyde/0.1 M cacodylate buffer + 1% tannic acid) overnight at 4 °C. Then, the organoids were postfixed with 1% osmium tetroxide/0.1 M cacodylate buffer, stained with 2% aqueous uranyl acetate (en bloc staining), dehydrated and embedded in Spurr’s resin. Ultrathin sections of 80 nm slices were cut using an ultramicrotome (Leica EM UC6, Hernalser Hauptstrasse, Vienna, Austria), collected on formvar-coated single-slot grids and examined under a transmission electron microscope (TEM, H-7500, Hitachi High-technologies, Tokyo, Japan) at 80 kV. The photoreceptor-specific sensory cilia and their compartments were investigated for week 20 retinal organoids.
5
Ultrastructural Analysis of Skeletal Muscle
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The skeletal muscles from drug-treated mice were fixed with 2.5% glutaraldehyde in 0.1 M phosphate buffer (pH 7.4), post-fixed for 1 h with 2% OsO4 dissolved in distilled water, dehydrated in a graded series of ethanol solutions, and embedded in Epon. Ultrathin sections were generated using an ultramicrotome (REICHERT ULTRACUT S, Leica Microsystems) and stained with uranyl acetate and lead citrate for examination under a transmission electron microscope (H-7500; Hitachi High-Technologies, Tokyo, Japan).
6
Transmission Electron Microscopy of Cell Spheres
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Spheres from PANC-1 and PK-1 cells were fixed with 2.5% glutaraldehyde in 0.1 M phosphate buffer (pH 7.4), then postfixed for 1 h with 2% OsO4 dissolved in distilled water. Cells were then dehydrated using an ethanol gradient, and embedded in Epon. Ultrathin sections were generated using an ultramicrotome and stained with uranyl acetate and lead citrate, as described previously, for examination under a transmission electron microscope (H-7500; Hitachi High-Technologies, Tokyo, Japan)17 (link),27 (link). Sphere formation was performed in triplicate for observation using TEM. To observe adherent cells using TEM, we cultured PANC-1 and PK-1 cells on cover slips in 24 well plates. The cells were fixed with 2.5% glutaraldehyde and then post-fixed for 30 min with 1% OsO4. After dehydration using graded ethanol, Epon in capsules was placed on the cover slip. Three days later, the cover slip was heated at 100 °C on a hot plate, and then the cells on the cover slip were attached to the Epon. Subsequent procedures were performed as described above.
7
Transmission Electron Microscopy of WYSV
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Leaf pieces from WYSV-infected wheat plants were fixed with glutaraldehyde, postfixed with 1% osmium tetroxide and embedded in araldite CY212 (Agar Scientific, Standsted, UK). Ultrathin sections were double-stained in 5% w/v uranyl acetate and 2% w/v lead citrate (pH 12) before observation and examined in a Hitachi model H-7500 transmission electron microscope (TEM, Hitachi High-Technologies, Tokyo, Japan) and photographed.
8
Characterizing Nanoparticles by TEM and Zetasizer
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The size and morphology of the NPs were characterized by transmission electron microscopy (TEM; H-7500, Hitachi High-Tech, Tokyo, Japan). The NPs were stained on dried formvar-coated 100-mesh copper grids at room temperature. All grids were further dried again for two days before imaging. The particle size and surface charge were examined using a Zetasizer (3000HS, Malvern Instruments Ltd., Worcestershire, UK) at room temperature.
9
Ultrastructural Analysis of Colon Tumor Tissues
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Colon tumor tissues and spheres produced by SS‐2 cells were fixed with 2.5% glutaraldehyde in 0.1 mol/L phosphate buffer (pH 7.4), and then post‐fixed for 1 hour with 2% OsO4 dissolved in distilled water. The samples were then dehydrated using an ethanol gradient, and embedded in Epon. Ultrathin sections were generated using an ultramicrotome and stained with uranyl acetate and lead citrate. Each section was examined with a transmission electron microscope (TEM, H‐7500; Hitachi High‐Technologies).
10
Characterization of Au and Au-Ag Nanoparticles
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TEM (H7500, Hitachi High-Technologies, Tokyo, Japan) was used to verify the size and morphology of the synthesized Au and Au-Ag NPs, and also to confirm the immobilization of the NPs on the cellulose paper. The distribution of the Au and Au-Ag NPs on cellulose chromatography paper was measured using SEM (JSM-6700F, JEOL, Tokyo, Japan). Au, Au-Ag NPs, and their immobilization on cellulose paper was also characterized with a UV-Vis spectrometer (Cintra 10e, GBC, Victoria, Australia). A zetasizer (Nano-HT, Malvern, UK) was employed to record the DLS measurement of the NPs in solution. An Orbital Shaking Incubator 740 (Cherng Huei Co., LTD., New Taipei City, Taiwan) was used for the bacterial cultures. An Ultrospec 10 Cell Density Meter (Amersham PLC, Little Chalfont, UK) was used to determine the optical density (O.D.) of the bacteria suspensions.
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