Sch 79797

Manufactured by Cayman Chemical

Sourced in United States

SCH 79797 is a chemical compound that functions as a selective inhibitor of the enzyme 5-lipoxygenase. It is used for research purposes in the study of inflammation and related biological processes.

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5 protocols using sch 79797

Purification and Characterization of Plasminogen and tPA

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Glu-Plasminogen was purified from human plasma as previously described [27 (link)]. Human tPA, which is produced in CHO cells and 95% in the two-chain form, was from Molecular Innovations. Lipopolysaccharide (LPS) serotype 055:B5 from E. coli was from Sigma-Aldrich. The TLR2 ligand, lipoteichoic acid (LTA) from S. aureus and the TLR9 ligand, ODN 1826, were from InvivoGen. Amiloride (AMD) was from Sigma-Aldrich. Mouse uPAR-specific antibody (cat. AF534) and control IgG (cat. AB105C) were from R&D Systems. α-Enolase-specific polyclonal antibody was from Invitrogen (cat. 3810T). Rabbit polyclonal antibody that targets the C-terminus of actin was from Sigma-Aldrich (cat. A2066). ε-Aminocaproic acid (εACA) was from MP Biomedicals. Aprotinin was from PanReac AppliChem. SCH 79797 was from Cayman Chemicals. The plasmin-specific chromogenic substrate, H-D-Val-Leu-Lys-p-nitroanilide (S-2251) and mouse uPA ATF were from Molecular Innovations.

Pontecorvi P., Banki M.A., Zampieri C., Zalfa C., Azmoon P., Kounnas M.Z., Marchese C., Gonias S.L, & Mantuano E. (2019). Fibrinolysis protease receptors promote activation of astrocytes to express pro-inflammatory cytokines. Journal of Neuroinflammation, 16, 257.

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Plasminogen Activation Biochemical Assay

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Enzymatically-active human tPA, which is produced in CHO cells and 95% in the two-chain form, and human EI-tPA, which carries the S478→A mutation and is 90% in the single-chain form, were from Molecular Innovations. Glu-Plasminogen was purified from human plasma as previously described.^{28 (link)} The purified Plasminogen preparations studied here were unresolved mixtures of the two major glycoforms. Plasmin (>10 International units/ mL), which was pre-activated with immobilized low molecular weight urokinase, was from Molecular Innovations. LPS serotype 055:B5 from E. coli was from Sigma-Aldrich. Dizocilpine (MK-801) was from Cayman Chemical. ε-aminocaproic acid (ƐACA) was from MP Biomedicals. Aprotinin was from PanReac AppliChem. SCH 79797 was from Cayman Chemicals and RWJ 56110 from R&D Systems. The PAR1 agonist peptide, TFLLR, and the control peptide, RLLFT, were from R&D Systems. The Plasmin-specific substrate, H-D-Val-Leu-Lys p-nitroanilide (S-2251), was from Molecular Innovations.

Zalfa C., Azmoon P., Mantuano E, & Gonias S.L. (2019). Tissue-type plasminogen activator neutralizes LPS but not protease-activated receptor-mediated inflammatory responses to plasmin. Journal of leukocyte biology, 105(4), 729-740.

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Evaluating Cellular Mechanisms in Skin Inflammation

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Tetraethyl thiuram (TTD), aristolochic acid (AA), silymarin (SLN), phorbol 12-myristate 13-acetate (PMA), porcine skin gelatin, collagen-I/IV, laminin, fibronectin, Hoechst stain, DNase 1, ficoll-paque, dextran and phosphatase inhibitor cocktail were obtained from Sigma-Aldrich (Bangalore, India). BSA, ethanol, dimethyl sulfoxide (DMSO; HPLC grade), Tween-20 and Hank’s balanced salt solution (HBSS) were purchased from HiMediaLaboratories, Pvt. Ltd. (Mumbai, India). SCH79797 (PAR-1 antagonist) and GB-83 (PAR-2 antagonist) were purchased from Cayman Chemicals (Michigan, USA). U0126 (MEK 1/2 inhibitor), antibodies against p-ERK, β-actin and cell lysis buffer were purchased from Cell Signaling Technology (Massachusetts, USA). HRP tagged anti-rabbit IgG and anti-mouse IgG were procured from Jackson ImmunoResearch (Philadelphia, USA). The rabbit polyclonal anti-citH3, rabbit polyclonal anti-H3, mouse monoclonal anti-myeloperoxidase (anti-MPO) and anti-PAD4 were obtained from Abcam (Cambridge, UK). All other chemicals and reagents used in this study are analytical grade.

Rudresha G.V., Urs A.P., Manjuprasanna V.N., Milan Gowda M.D., Jayachandra K., Rajaiah R, & Vishwanath B.S. (2021). Echis carinatus snake venom metalloprotease-induced toxicities in mice: Therapeutic intervention by a repurposed drug, Tetraethyl thiuram disulfide (Disulfiram). PLoS Neglected Tropical Diseases, 15(2), e0008596.

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Purification and Characterization of HuPK and rBbKI

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HuPK was purified as previously described [14 (link)] and the specific activity was found to be 80 U/mg. Recombinant Bauhinia bauhinioides kallikrein inhibitor (rBbKI) was obtained as described [15 (link)]. ADP, ATP-standard, and luciferin-luciferase were from Chrono-Log (Chester, PA, USA). Human α-thrombin (200 NIH units/mg) was from Sigma (St. Louis, MO, USA). The PAR-1 antagonist SCH 79797 was from Cayman Chemical (Ann Arbor, MI, USA); abciximab was from Eli Lilly and Company (Indianapolis, IN, USA), while Fluo-4-AM was from Thermo Fisher Scientific Corporation (Waltham, MA, USA). TLCK was purchased from Merck KGaA /Calbiochem (Darmstadt, Germany).

Ottaiano T.F., Andrade S.S., de Oliveira C., Silva M.C., Buri M.V., Juliano M.A., Girão M.J., Sampaio M.U., Schmaier A.H., Wlodawer A., Maffei F.H, & Oliva M.L. (2017). Plasma Kallikrein enhances platelet aggregation response by subthreshold doses of ADP. Biochimie, 135, 72-81.

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Inflammatory Mediator Signaling Pathway

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Tetraethylthiuram (TTD), aristolochic acid (AA), silymarin (SLN), phorbol 12-myristate 13acetate (PMA), porcine skin gelatin, collagen type 1, laminin, fibronectin, Hoechst stain, DNase 1, ficoll-paque, dextran and phosphatase inhibitor cocktail were obtained from Sigma-Aldrich (Bangalore, India). BSA, ethanol, dimethyl sulfoxide (DMSO; HPLC grade), Tween-20 and Hank's balanced salt solution (HBSS) were purchased from HiMediaLaboratories, Pvt. Ltd.
(Mumbai, India). SCH79797 (PAR-1 antagonist) and GB-83 (PAR-2 antagonist) were purchased from Cayman Chemicals (Michigan, USA). U0126 (MEK 1/2 inhibitor), antibodies against p-ERK and β-actin were purchased from Cell Signaling Technology (Massachusetts, USA). HRP tagged anti-rabbit IgG and anti-mouse IgG were procured from Jackson ImmunoResearch (Philadelphia, USA). The rabbit polyclonal anti-citH3, rabbit polyclonal anti-H3, mouse monoclonal antimyeloperoxidase (anti-MPO) and anti-PAD4 were obtained from Abcam (Cambridge, UK). All other chemicals and reagents used in this study are analytical grade.

Rudresha G.V., Urs A.P., Manjuprasanna V.N., Gowda M.D.M., Jayachandra K., Rajaiah R., & Vishwanath B.S. (2020). Echis carinatussnake venom metalloprotease-induced toxicities in mice: therapeutic intervention by a repurposed drug, tetraethylthiuram disulfide (disulfiram).

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