Colors of basidiomata and sclerotia were described according to the color identification chart of the Royal Botanic Garden Edinburgh (Flora of British Fungi) (Anonymous, 1969 ). Basidiospores from fresh specimens were mounted in water for light microscopic examination. About 30 basidiospores were randomly chosen for determination of length and width excluding the apiculus. Surface features of basidiomata and sclerotia were observed by phase-contrast microscopy and scanning electron microscopy (SEM). For SEM, basidiomata and sclerotia were cut on a piece of double-sided adhesive tape attached to a specimen holder and then coated with platinum-palladium using a JFC-1100 Ion Sputter (JEOL, Tokyo, Japan). They were examined using a JSM-T330A SEM (JEOL) operating at 10 kV.
Jsm t330a sem
Manufactured by JEOL
Sourced in Japan
The JSM-T330A is a scanning electron microscope (SEM) manufactured by JEOL. It is designed for high-resolution imaging and analysis of a wide range of materials. The JSM-T330A operates at an accelerating voltage of up to 30 kV and can achieve a resolution of up to 3.5 nm. The microscope is equipped with a tungsten filament electron source and features a range of imaging and analysis capabilities.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using jsm t330a sem
1
Basidiomata and Sclerotia Morphology Analysis
2
Frost-columnar Colony Morphology of G. antarctica
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
One loopful of cells of G. antarctica S4B was inoculated on a frozen PDA plate including 0.05% (w/v) red food color (Kyoritsu Foods Co., Tokyo, Japan) and was incubated at −1 °C for two months. Three plates were used for this experiment in triplicate. Surface features of frost-columnar colonies were observed by scanning electron microscopy (SEM). Frost-columnar colonies were lyophilized and coated with platinum-palladium using JFC-1100 Ion Sputter (JEOL, Tokyo, Japan). They were examined using JSM-T330A SEM (JEOL, Tokyo, Japan) operating at 15 kV.
3
SEM Imaging of Ferroptotic MCA Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
SEM imaging was performed on non-coated and coated ferroptotic MCA cells. Specimen preparation was performed as stated by Chen et al [79] (link) . First the MCA cells were treated with the according cell death inducer (RSL3) and left for 24h. After performing the LBL-coating the cells were deposited onto poly-L-lysine coated glasses overnight. Subsequently cells were fixed with 3% glutaraldehyde (Sigma-Aldrich, St. Louis, USA) in PBS buffer for 1 h and post fixed with 1% osmium tetroxide (Sigma-Aldrich) for another hour in the dark at room temperature.
The cells are then washed three times with distilled water, and the fixed cells were dehydrated in graded ethanol solutions. Prior to SEM observation, the glasses containing the cells were dried in a lyophilizer on an aluminium stage covered with double-sided carbon tape and sputtercoated with a 15 nm thick gold layer (Bal-tec SCD050 Sputter Coater, MA, USA). SEM topography measurements were conducted using a JSM-T330A SEM (JEOL) at the operating voltage of 25 kV.
The cells are then washed three times with distilled water, and the fixed cells were dehydrated in graded ethanol solutions. Prior to SEM observation, the glasses containing the cells were dried in a lyophilizer on an aluminium stage covered with double-sided carbon tape and sputtercoated with a 15 nm thick gold layer (Bal-tec SCD050 Sputter Coater, MA, USA). SEM topography measurements were conducted using a JSM-T330A SEM (JEOL) at the operating voltage of 25 kV.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!