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Dulbecco s modified eagle s medium dmem

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Dulbecco's modified Eagle's medium (DMEM) is a cell culture medium commonly used for the in vitro cultivation of a variety of cell types, including mammalian cells. It provides the necessary nutrients and growth factors required for cell proliferation and maintenance.

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Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (14 mentions) Fetal bovine serum (fbs), by HiMedia (10 mentions) 3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by HiMedia (6 mentions) Antibiotic antimycotic solution, by HiMedia (4 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (4 mentions) Fetal bovine serum (fbs), by Merck Group (4 mentions) Phosphate buffered saline (pbs), by HiMedia (4 mentions) Trypsin edta solution, by HiMedia (3 mentions) Acridine orange, by Merck Group (3 mentions) Dimethyl sulfoxide (dmso), by Merck Group (3 mentions) Methanol, by Merck Group (2 mentions) Chrysin, by Merck Group (2 mentions) Penicillin streptomycin, by HiMedia (2 mentions) Ethidium bromide, by HiMedia (2 mentions) Formalin, by HiMedia (2 mentions) Insulin, by HiMedia (2 mentions) Trypsin, by Thermo Fisher Scientific (2 mentions) Propidium iodide, by Merck Group (2 mentions) Trypsin edta, by HiMedia (2 mentions) Sodium hydroxide (naoh), by Merck Group (2 mentions)

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DMEM medium (8 citations)

40 protocols using dulbecco s modified eagle s medium dmem

1

Lipid-based Paclitaxel Nanocarrier Formulation

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1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and
1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) with purity > 99% were purchased from
Avati Polar Lipids, Inc (Alabaster, USA). Paclitaxel (purity > 99%) was purchased from Fresenius
Kabi India Pvt. Ltd. (India). Taxol was purchased from Cipla Ltd. (India) and Abraxane was purchased
from Biocon (India). Dialysis membrane (molecular wt. cutoff 5000–10000), Dulbecco's Modified Eagles
Medium (DMEM), fetal bovine serum (FBS), antibiotic antimycotic solution, sodium azide, phosphate
buffered saline (PBS) and trypsin-EDTA solution were purchased from Himedia Laboratories Pvt Ltd.,
Mumbai (India). Sulphorhodamine-B was purchased from Sigma Aldrich, Mumbai (India). Rhodamine-6G was
purchased from Anaspec Inc. (San. Jose, CA, USA) and BCA protein assay kit was purchased from Thermo
Scientific, Pierce (Rockford, Il, USA). Mouse TNF-α, mouse IL-1β and mouse IL-6 elisa kits were
purchased from RayBiotech, Inc., USA. High pressure liquid chromatography (HPLC) grade methanol and
chloroform were purchased from Merck, Mumbai (India). All the tissue culture plates and tissue
culture flasks were purchased from NUNC (USA). High purity water purified by a Milli Q Plus water
purifier system (Milli pore, USA), with a resistivity of 18.2 MΩcm, was used in all experiments.
Joshi N., Shirsath N., Singh A., Joshi K.S, & Banerjee R. (2014). Endogenous lung surfactant inspired pH responsive nanovesicle aerosols: Pulmonary compatible and site-specific drug delivery in lung metastases. Scientific Reports, 4, 7085.
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2

Chrysin and Sodium Selenite Cytotoxicity Study

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Chrysin (97% pure) was purchased from Sigma-Aldrich Chemical Co. (St. Louis, MO), sodium selenite (Na2SeO3) was obtained from LOBA Chemie (Mumbai, India), and Dulbecco’s modified Eagle’s medium (DMEM) and the antibiotics streptomycin and penicillin were obtained from HiMedia (Mumbai, India). Antibodies for m-calpain, Lp82, EGR-1, COX-1, caspase-3, caspase-8, caspase-9, and β-actin were purchased from Sigma-Aldrich Chemical Co. and Cell Signaling Technologies (CST; Danvers, MA). Anti-rabbit immunoglobulin (IgG) secondary antibody was obtained from Genei (Bengaluru, India). All other chemicals and reagents were of analytical grade. Milli-Q water (Millipore, Bengaluru, India) was used throughout the experiments.
Sundararajan M., Thomas P.A., Teresa P.A., Anbukkarasi M, & Geraldine P. (2016). Regulatory effect of chrysin on expression of lenticular calcium transporters, calpains, and apoptotic-cascade components in selenite-induced cataract. Molecular Vision, 22, 401-423.
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3

Cell Line Maintenance for Cancer Research

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In-vitro cancer cell lines K562, A549, and U937 cancer cells were obtained from National Centre for Cell Science (NCCS), Pune, India and EAC, Sarcoma-180, B16F10 cancer cells were collected from Chittaranjan National Cancer Research Institute (CNCRI), Kolkata, India. The in-vitro cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM), (HiMedia India) supplemented with 10% FBS at 37°C in CO2 incubator in an atmosphere of humidified 5% CO2 and 95% air. The cells were maintained routinely in subcultures in tissue culture flasks. The EAC, Sarcoma-180 and B16F10 cells were maintained in the peritoneal cavity of mice by injecting 0.1ml at fluid cell every 7 days. Tumor cell counts were done in a Neubaner hemocytometer using the trypan blue dye exclusion method. Cell viability was always found to be 95% or more (Sangameswaran et al., 2012). Tumor cell suspensions were prepared in phosphate buffered saline (PBS).
Debnath S., Karan S., Debnath M., Dash J, & Chatterjee T.K. (2017). Poly-L-Lysine Inhibits Tumor Angiogenesis and Induces Apoptosis in Ehrlich Ascites Carcinoma and in Sarcoma S-180 Tumor. Asian Pacific Journal of Cancer Prevention : APJCP, 18(8), 2255-2268.
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4

Cell Viability Assay Protocol

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Trypsin was procured from Gibco, USA, while Phosphate Buffered Saline (PBS), Fetal Bovine Serum (FBS), Dulbecco's Modified Eagles Medium(DMEM), 3-[4, 5-dimethylthiazol-2-yl]-2, 5 diphenyltetrazolium bromide (MTT), penicillin-streptomycin, and ethidium bromide were purchased from Hi-Media Laboratories, Mumbai, India. Rest of the chemicals employed in the study was analytical grade.
Sathiyamoorthy J, & Sudhakar N. (2018). In vitro Cytotoxicity and Apoptotic Assay in HT-29 Cell Line Using Ficus hispida Linn: Leaves Extract. Pharmacognosy Magazine, 13(Suppl 4), S756-S761.
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5

Tissue Sample Collection and Analysis

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Cryovials for tissue sample collection (Tarsons, Cat: 523040), reagents required for RNA isolation, namely, TRI reagent (Cat: T9424), Chloroform (Cat: V800116), isopropanol (Cat: V800228), formaldehyde (Cat: V800189), MOPS (Cat: M1254), DEPC (Cat: D5758) and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) reagent (Cat: M2128), were purchased from Sigma-Aldrich. Mayer’s hematoxylin (Himedia Cat: S058) and eosin (Himedia Cat: S007) stains for histological staining, LDH Cytotoxicity Assay Kit (BioVision, Cat: K311-400), acridine orange (AO; Cat: A6014), propidium iodide (Cat: P4864), and all quantitative reverse transcription (qRT)-polymerase chain reaction (PCR) primers were purchased from Sigma-Aldrich. Fetal bovine serum (FBS; Gibco, Cat: 10270), Dulbecco’s modified Eagle’s medium (DMEM; Cat: SH30243.01), Antibiotic Antimycotic Solution (Cat: A002), Trypsin-EDTA (Himedia Labs, Cat: TCL007), mortar, pestle, and micropestle for grinding tissues were purchased from local vendors.
Setty Balakrishnan A., Nathan A.A., Kumar M., Ramamoorthy S, & Ramia Mothilal S.K. (2017). Withania somnifera targets interleukin-8 and cyclooxygenase-2 in human prostate cancer progression. Prostate International, 5(2), 75-83.
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6

Synthesis and Characterization of ASPIONs

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FeCl3 anhydrous, FeCl2∙4H2O, NaOH, 35% HCl (All from Merck, Germany/India) and Sodium Alginate Polysaccharide (Sigma Aldrich, India) were used for the preparation of ASPIONs. NIR emitting dye (Atto 700) was procured from Sigma Aldrich, India for optical imaging. Deionised Milli Q water of resistivity 18.2 MΩ was used throughout the work. All other chemicals and reagents used were of analytical grade.
For the cell culture study, Dulbecco’s Modified Eagle’s Medium (DMEM) (Himedia Laboratories Pvt. Ltd, Mumbai, India), sodium bicarbonate, Gentamicin (Himedia, Germany), Amphotericin B solution (Sigma-Aldrich, Germany), Fetal bovine serum (FBS), antibiotic–antimycotic mix (Himedia, India) and 3-(4, 5-dimethylthiazol-2-yl)-2.5-diphenyltetrazolium bromide (MTT) were used. HepG2 carcinoma cells were supplied from National Centre for Cell Science (NCCS, Pune, India). Carbon tetrachloride (CCl4) purchased from Spectrum Reagents & Chemicals Ltd, India and olive oil were used to develop fibrosis in rodents.
Nazeer S.S., Saraswathy A., Nimi N., Santhakumar H., Radhakrishnapillai Suma P., Shenoy S.J, & Jayasree R.S. (2023). Near infrared-emitting multimodal nanosystem for in vitro magnetic hyperthermia of hepatocellular carcinoma and dual imaging of in vivo liver fibrosis. Scientific Reports, 13, 12947.
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7

Epalrestat-Mediated Neuroprotection Assay

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Epalrestat, 3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), 2',7'-diacetyl dichlorofluorescein (DCFHDA), Rhodamine 123 (Rh-123), and fetal bovine serum (FBS) were purchased from Sigma Aldrich, USA. Dulbecco’s modified Eagle’s medium (DMEM), ethylene diamine tetra acetic acid (EDTA), trypsin, Folin’s Ciocalteu reagent (FCR), trichloro acetic acid (TCA), and 4', 6-diamidino-2-phenylindole (DAPI) were purchased from Himedia, India. Anti-GSK3-β, anti-total tau, anti-β-actin and anti-rabbit secondary antibodies were purchased from Cell Signaling, USA.
Lingappa S., Shivakumar M.S., Manivasagam T., Somasundaram S.T, & Seedevi P. (2021). Neuroprotective Effect of Epalrestat on Hydrogen Peroxide-Induced Neurodegeneration in SH-SY5Y Cellular Model. Journal of Microbiology and Biotechnology, 31(6), 867-874.
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8

Multifunctional Nanoparticle Synthesis and Characterization

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Magnesium nanoparticles with APS < 12 nm and purity of >99% were procured from Intelligent Materials Pvt. Ltd. (India). Ferric chloride tetrahydrate, ferrous chloride hexahydrate, transferrin (Tf), N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide (EDC.HCl), glutathione (GSH), PAMAM–NH2-G4 (G4) dendrimer 10 wt% in methanol (Mw 14214.7 Da, 64 end groups), N-hydroxysuccidimide (NHS), N,N-Diisopropylethylamine (DIEA), sodium bicarbonate (NaHCO3), phosphate buffer saline (PBS), Hoechst 33250 dye, were purchased from Sigma-Aldrich, USA. McCoy’s 5 A medium, fetal bovine serum (FBS), and penicillin and streptomycin (Pen-Strep) were purchased from Sigma-Aldrich, USA. Anti-epithelial cell adhesion molecule (EpCAM) antibody was purchased from Bioss Antibodies, USA. Dulbecco’s Modified Eagle’s medium (DMEM) and trypsin were purchased from Himedia, India. Cyanine 5 mono NHS ester was procured from GE Healthcare, UK. Ultrapure water (MilliQ) used in this work was acquired from Direct-Q® 3 water purification system, Merck Millipore, Germany. All other chemicals were of analytical grade and used without further purification.
Wavhale R.D., Dhobale K.D., Rahane C.S., Chate G.P., Tawade B.V., Patil Y.N., Gawade S.S, & Banerjee S.S. (2021). Water-powered self-propelled magnetic nanobot for rapid and highly efficient capture of circulating tumor cells. Communications Chemistry, 4, 159.
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9

NIH 3T3 Cell Culture and Analysis

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NIH 3T3 (Mouse Embryonic Fibroblast) cell line was purchased from NCCS, Pune, India. The cells were maintained in Dulbecco's Modified Eagle's Medium (DMEM) which was obtained from HiMedia, India. Other chemicals such as Fetal Bovine Serum (FBS), Trypsin-EDTA solution, Antibiotic Penicillin-Streptomycin, Crystal violet, and Trypan blue were also obtained from Himedia, India. Acetic acid was purchased from Merck, US.
Glutamax was purchased from Thermo Fisher Scientific, US. Acetic acid was purchased from Merck, US. Fluorescein isothiocyanate-Phalloidin (FITC-Phalloidin) and 4', 6-diamidino-2phenylindole (DAPI), were obtained from ThermoFisher Scientific, US. FITC Annexin V Apoptosis Detection Kit I (BD Pharmingen™, Cat no. 556547) was obtained from BD Biosciences, US. Cell culture inserts of polyester (PET) membrane, 8 µm pore size, were purchased from BD Falcon. Ethanol used in this study is of HPLC grade from Commercial Alcohols, Greenfield Global, Canada. All the reagents and chemicals are of analytical grade.
Kar N., & Bellare J. (2021). Ethanol pre-exposure enhances alcohol-seeking behavior at cellular level by chemoattraction and exhibits bleb-driven cellular stress response in uniform ethanol concentration.
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10

Synthesis of Iron Nitrate Nanocomposite

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Fe(NO3)3·9H2O was purchased from Qualigens India. Carbon nanoplate powder was obtained from Cabot Corporation, USA. DAPI and trypsin were purchased from Sigma-Aldrich, USA. DMSO, dulbecco's modified eagle's medium (DMEM), fetal bovine serum (FBS), MTT, DAPI, Propidium Iodide (PI), trypan blue, phosphate buffer saline (PBS) and citric acid (C6H8O7) were obtained from Himedia, India. All chemical reagents were used without further purification.
AlSalhi M.S., Devanesan S., Shanmugam P., Kim Y.O., Kwon J.T, & Kim H.J. (2019). Synthesis and biocompatible role of hierarchical structured carbon nanoplates incorporated α-Fe2O3 nanocomposites for biomedical applications with respect to cancer treatment. Saudi Journal of Biological Sciences, 27(2), 588-593.
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