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2 protocols using deoxycholic acid

1

Comprehensive Bile Acid Standards Analysis

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Glycholithocholic acid (GLCA) standards were purchased from Toronto Research Chemicals; Cholic acid (CA), lithocholic acid (LCA), deoxycholic acid (DCA), ursodeoxycholic acid (UDCA), chenodeoxycholic acid (CDCA), taurocholic acid (TCA), glychocholic acid (GCA), taurolithocholic acid (TLCA), taurodeoxycholic acid (TDCA), tauroursodeoxycholic acid (TUDCA), taurochenodeoxycholic acid (TCDCA), glychodeoxycholic acid (GDCA), glychochenodeoxycholic acid (GCDCA), glychoursodeoxycholic acid (GUDCA) standards, and isotope-labeled internal standard deuterocholic acid (d4-CA), deuterodeoxycholic acid (d4-DCA), deuteroglychocholic acid (d4-GCA), deuterated ursodeoxycholic acid (d4-UDCA), and deuterated lithocholic acid (d4-LCA) were purchased from Sigma Aldrich; Chromatographic pure acetonitrile and chromatographic pure methanol were purchased from Merck, Germany; Chromatographic grade formic acid was purchased from Shanghai Aladdin.
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2

Comprehensive Bile Acid Analysis by LC-MS

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LC-MS grade water, methanol and acetonitrile were purchased from Thermo Fisher Scientific (Sydney, Australia). Chenodeoxycholic acid (2,2,4,4-d4) (CDCA-d4), cholic acid (CA), chenodeoxycholic acid (CDCA), deoxycholic acid (DCA), lithocholic acid (LCA), ursodeoxycholic acid (UDCA), glycodeoxycholic acid (GDCA), glycocholic acid (GCA), glycolithocholic acid (GLCA), taurochenodeoxycholic acid (TCDCA), taurocholic acid (TCA), taurodeoxycholic acid (TDCA), tauroursodeoxycholic acid (TUDCA) were purchased from Toronto Research Chemicals (Toronto, Canada). lithocholic acid (2,2,4,4-d4) (LCA-d4) and taurodeoxycholic acid (2,2,4,4-d4) (TDCA-d4) were obtained from Cambridge Isotope Laboratories, Inc. (Massachusetts, USA). deoxycholic acid (2,2,4,4-d4) (DCA-d4) was purchased from Sigma-Aldrich (NSW, Australia).
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