Uv 3150 spectrophotometer

NADPH was purchased from Sigma. TPE-cyc was synthesized according to literature procedures (Cheng et al., 2019 (link)). ¹H NMR and ¹³C NMR spectra were recorded on a Bruker AvanceⅢ-400 spectrometry. The 2D NOESY NMR spectra were recorded on a Bruker Avance DMX 600 spectrophotometer with TMS as the internal reference. UV-vis-NIR spectra were taken on a Shimadzu UV-3150 spectrophotometer. The fluorescence experiments were measured on an RF-5301 spectrofluorophotometer (Shimadzu Corporation, Japan). The isothermal titration calorimetry (ITC) experiments were performed on a VP-ITC micro-calorimeter (Microcal, United States). The cell images were taken by a confocal laser scanning microscopy (CLSM, Radiance2100, Bio-Rad) with a 100 × oil immersion lens. Flow cytometry measurements were conducted using a FACSCalibur flow cytometer (BD FACSCalibur).

Absorption data were recorded on a Shimadzu UV-3150 spectrophotometer using a standard cell with a path length of 10 mm. TEM measurements for (6, 5)-enriched SWCNT/fullerodendron/Pt(II) nanocomposites were conducted using a JEM-2100 transmission electron microscope (80 kV). The specimens for the measurements were prepared by applying a few drops of sample solution onto a holey carbon-coated copper grid, and then evaporating the solvent. Atomic force microscopy (AFM) observation was carried out using a Seiko SPA 400-DFM. Samples for observation were prepared by placing a drop of the aqueous specimen on freshly cleaved mica, then allowing each drop to dry. X-ray photoelectron spectroscopy (XPS) measurements were conducted with a JPS-9030 spectrometer using a monochromatic Al Kα X-ray source with a pass energy of 20 eV. The spectra were adjusted with reference to the C 1 s peak at 284.6 eV. Three-dimensional fluorescence spectra data were obtained using a spectrofluorometer (Shimadzu, NIR-PL system). (6, 5)-enriched SWCNTs were purchased from Sigma-Aldrich Co. All other reagents were purchased from Kanto Kagaku Co., Ltd, Sigma-Aldrich Co., and Tokyo Kasei Co., Ltd. All chemicals were used as received. Fullerodendron was prepared according to the reported procedure29 .

Transmission electron microscopy (TEM) images were obtained by a JSM-2100Plus (JEOL, Tokyo, Japan) microscope at an accelerating voltage of 100.0 kV, equipped with an energy-dispersive spectroscopy (EDS) detector. The UV–vis absorption spectra were recorded with a Shimadzu UV3150 spectrophotometer (Kyoto, Japan). Fluorescence spectra were measured with a Fluoromax–4 Spectrofluorometer (HORIBA, Kyoto, Japan).

The absorption data were recorded on a UV-3150 spectrophotometer (Shimadzu, Tokyo, Japan) using a standard cell with a path length of 10 mm. SEM measurements for the composites were conducted using a JEM-2100 (JEOL Ltd., Tokyo, Japan). Specimens for the measurements were prepared by applying a few drops of the sample solution onto a dedicated grid, and then evaporating the solvent. Raman spectra were obtained on a JASCO NRS-5100 (JASCO Co., Japan) using laser excitation at 532 nm.

Photoabsorption spectra of solutions and films were observed with a SHIMADZU UV-3150 spectrophotometer. Fluorescence spectra of solutions, films, and solids were measured with a HITACHI F-4500 spectrofluorometer.