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1 ethyl 3 3 dimethylaminopropyl carbodiimide

Manufactured by Merck Group
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1-ethyl-3-(3-dimethylaminopropyl) carbodiimide is a lab equipment chemical compound used for protein coupling reactions. It is a water-soluble carbodiimide that can be used to activate carboxyl groups for conjugation with primary amines.

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Lab products found in correlation

N hydroxysuccinimide, by Merck Group (75 mentions) Sodium hydroxide (naoh), by Merck Group (20 mentions) Dimethyl sulfoxide (dmso), by Merck Group (16 mentions) Phosphate buffered saline (pbs), by Merck Group (15 mentions) 4 dimethylaminopyridine, by Merck Group (12 mentions) Methanol, by Merck Group (11 mentions) Hydrochloric acid (hcl), by Merck Group (10 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (9 mentions) Dimethylformamide, by Merck Group (8 mentions) Succinic anhydride, by Merck Group (7 mentions) N hydroxysulfosuccinimide, by Merck Group (7 mentions) 1 octadecene, by Merck Group (7 mentions) Fluorescein isothiocyanate (fitc), by Merck Group (7 mentions) Chitosan, by Merck Group (6 mentions) Sulfuric acid, by Merck Group (6 mentions) Triethylamine, by Merck Group (6 mentions) Oleic acid, by Merck Group (6 mentions) Acrylic acid, by Merck Group (6 mentions) Ethanolamine, by Merck Group (6 mentions) Bovine serum albumin (bsa), by Merck Group (6 mentions)

149 protocols using 1 ethyl 3 3 dimethylaminopropyl carbodiimide

1

Chitosan-based Nanomaterial Synthesis

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Chitosan, 1-Ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC), N-hydroxysuccinimide (NHS), succinic anhydride, Pluronic® F127 and 4-dimethylaminopyridine (DMAP) were purchased from Aldrich (Sigma Aldrich, St. Louis, MO, USA). 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) and 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) were purchased from Avanti Polar Lipids (Alabaster, AL, USA). All other reagent grade chemicals and solvents were used as received and were obtained from commercial sources.
Olguín Y., Campos C., Catalán J., Velásquez L., Osorio F., Montenegro I., Madrid A, & Acevedo C. (2017). Effects of Liposomes Contained in Thermosensitive Hydrogels as Biomaterials Useful in Neural Tissue Engineering. Materials, 10(10), 1122.
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2

Electrochemical Biosensor for Fluorescein Detection

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The experimental material consisted of a screen-printed carbon electrode (SPCE) as a sensing platform, which was purchased from Zensor R&D (Taichung, Taiwan). Figure 1 shows our previous developed biosensor with optimizations to enhance signal detection [17 (link)]. The following materials and reagents were purchased from Sigma-Aldrich (Sigma-Aldrich St. Louis, MO, USA): phosphate-buffered saline (PBS), carboxymethyl dextran sodium salt (CMD-Na), 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), N-hydroxysulfosuccinimide (NHS), ethanolamine, potassium chloride (KCl), potassium ferricyanide (Fe(CN)63−), hydrogen peroxide (H2O2), 3, 3′, 5, 5′-tetramethylbenzidine (TMB), and diethyl pyrocarbonate (DEPC). NeutrAvidin protein was supplied by Thermo Fisher Scientific (Waltham, MA, USA). Antifluorescein horseradish peroxidase (HRP), a 40,000 dalton protein, was acquired from Abcam (Cambridge, UK). MES-free acid monohydrate, hydroxymethyl-aminomethane (Tris), and ethylenediaminetetraacetic acid (EDTA) were purchased from Amresco (Amresco Inc., Solon, OH, USA). Sodium chloride (NaCl) was obtained from Promega Corporation (Madison, WI, USA). We designed a biotinylated ssDNA probe (bioreceptor probe), fluorescein (FITC) ssDNA probe (detector probe), and artificial mimic targeting sequence using Genomics (Taipei, Taiwan).
Chiou Y.E., Yu K.J., Pang S.N., Yang Y.L., Pang S.T, & Weng W.H. (2022). A Highly Sensitive Urinary Exosomal miRNAs Biosensor Applied to Evaluation of Prostate Cancer Progression. Bioengineering, 9(12), 803.
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3

Functionalized Nanoparticle Synthesis Protocol

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Citric acid, thiourea, urea, hexadecylamine, 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC), N-hydroxysuccinimide (NHS), anhydrous dimethylformamide (DMF), and 0.22 μm pore size syringe filters containing a hydrophilic polyethersulfone (PES) membrane were purchased from Merck. Ethanol (EtOH), sodium hydroxide, ammonia, hydrochloric acid, and calcium chloride were purchased from Associated Chemical Enterprises (South Africa). Phenanthrene (PHE) standard (≥98% purity) was purchased from Supelco. Deionised water (DI, 9.2 μS cm−3) from a Milli-Q water purification system (Millipore, Bedford, MA, USA) was employed to prepare all the solutions. SnakeSkin™ 3.5 kDa MCOW dialysis tubing was purchased from Thermo Fisher Scientific (South Africa).
Kubheka G., Adeola A.O, & Forbes P.B. (2022). Hexadecylamine functionalised graphene quantum dots as suitable nano-adsorbents for phenanthrene removal from aqueous solution. RSC Advances, 12(37), 23922-23936.
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4

Carbodiimide-Mediated Biomolecule Conjugation

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3-Mercaptopropionic acid (3MPA), N-hydroxysulphosuccinimide (NHSS), 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC), sodium hydroxide (NaOH), and ethanolamine chloride (EA) were purchased from Merck and used without further purification. A 0.1 M 2-(N-morpholino)ethane-sulfonic acid (MES) buffer (Sigma–Aldrich) solution was adjusted with NaOH 1 M at pH 4.8–4.9. Phosphate-buffered saline (PBS) solution (phosphate buffer of 10 mM, KCl 2.7 mM, NaCl 137 mM, pH 7.4) was prepared by dissolving a PBS tablet (Sigma–Aldrich) in 200 mL of HPLC water. The solution was filtered on a Corning 0.22 μm polyethersulfone membrane before use. Potassium Ferrocyanide (K4[Fe(CN)6]), Potassium Ferricyanide (K3[Fe(CN)6]), and sulfuric acid (H2SO4, 95–98%) were purchased from Sigma–Aldrich (now Merck). Solutions used for sample functionalization were prepared using HPLC water (Fluka/C. Erba), while solutions for electrochemical measurement (H2SO4, NaOH) were prepared in Milli-Q water (18.2 MΩ cm−1, Millipore, Bedford, MA, USA).
Tricase A., Imbriano A., Ditaranto N., Macchia E., Picca R.A., Blasi D., Torsi L, & Bollella P. (2022). Electrochemical and X-ray Photoelectron Spectroscopy Surface Characterization of Interchain-Driven Self-Assembled Monolayer (SAM) Reorganization. Nanomaterials, 12(5), 867.
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5

Trastuzumab-Epirubicin Nanoparticle Synthesis

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DOPE was purchased from Lipoid AG. Chol, N‐hydroxycarbidiimide (NHS), 3‐(4,5‐cimethylthiazol‐z‐yl)‐2,5‐diphenyltetrazolium bromide (MTT), 1‐ethyl‐3‐(3‐dimethyl aminopropyl) carbodiimide (EDC), trypsin‐EDTA, were obtained from Merck. Potassium dihydrogen phosphate, dimethyl sulfoxide (DMSO), analytical grade ethanol and acetone were purchased from Sigma‐Aldrich Co. Trastuzumab (Mw 145.5 kDa) was supplied by LuyePharma. Epirubicin was purchased from Sigma‐Aldrich Co.
Khaleseh F., Hemmati Azandaryani A., Fathian Kolahkaj F., Khazaei M, & Derakhshandeh K. (2021). Enhancement of in vitro antitumour activity of epirubicin in HER2+ breast cancer cells using immunoliposome formulation. IET Nanobiotechnology, 15(3), 257-265.
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6

Fluvoxamine-loaded Chitosan Nanoparticles

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Fluvoxamine (FVM) was gifted by Pharmevo Pharmaceuticals Pvt. Ltd, Lahore, Pakistan. Carboxymethyl chitosan (CMC) with Case number 83512-85-0 purchased from Glentham Life Sciences, Corsham, UK. CMC is white to light yellow or pale beige powder with 80% degree of deacetylation. Span 60, cholesterol, arachidonic acid (AA), methanol, 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC), N Hydroxysuccinimide (NHS), ethanol and potassium dihydrogen phosphate were purchased from Merck KGaA, Darmstadt, Germany.
Gulshan S., Shah S., Shah P.A., Irfan M., Saadullah M., Abbas G., Hanif M., Rasul A., Ahmad N., Mahmood A., Basheer E., Habib M.O., Alotaibi H.F., Obaidullah A.J., Alsabhan J.F, & Alwassil O.L. (2023). Development and Pharmacokinetic Evaluation of Novasomes for the Trans-nasal Delivery of Fluvoxamine Using Arachidonic Acid-Carboxymethyl Chitosan Conjugate. Pharmaceutics, 15(9), 2259.
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7

Gelatin-Based Hydrogel Synthesis

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Gelatin A from porcine
skin, GG (Gelzan CM Gelrite, Mw 1000 g
mol–1), spermidine trihydrochloride (SPD), sucrose,
adipic dihydrazide (ADH), carbodihydrazide (CDH), dimethyl sulfoxide
(DMSO), ethylene glycol, 1-ethyl-3-[3-(dimethylamino)-propyl]-carbodiimide
(EDC), hydroxylamine hydrochloride, N-hydroxybentzotriazole
(HOBt), 4-hydroxybenzaldehyde, deuterium oxide (99.9 atom % D, contains
0.05 wt % 3-(trimethylsilyl)-propionic-2,2,3,3-d4 acid, sodium salt),
hydrochloric acid (HCl), sodium hydroxide (NaOH), sodium chloride
(NaCl), and sodium periodate (NaIO4) were purchased from
Sigma-Aldrich (St. Louis, MO, USA). Dialysis membrane (Spectra/Por
12–14 kDa) was purchased from Spectrum Laboratories (Rancho
Dominguez, CA, USA).
Koivisto J.T., Gering C., Karvinen J., Maria Cherian R., Belay B., Hyttinen J., Aalto-Setälä K., Kellomäki M, & Parraga J. (2019). Mechanically Biomimetic Gelatin–Gellan Gum Hydrogels for 3D Culture of Beating Human Cardiomyocytes. ACS Applied Materials & Interfaces, 11(23), 20589-20602.
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8

Oligonucleotide Probes Immobilization for Biosensing

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1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide, N-hydroxy sulfosuccinimide, dithiothreitol, N,N-dimethylformamide, potassium permanganate, sulfuric acid, phosphoric acid (H3PO4), and Luria Bertani agar were purchased from Sigma-Aldrich (USA). Hydrogen tetrachloroaurate, trisodium citrate, and graphite flakes were purchased from Acros Organics (USA). Immobilization and purification of the modified oligonucleotide probes onto the nanoparticle platforms was done using NAP-10 sephadex columns (G-25, GE Healthcare) and with a high-speed centrifuge (MIKRO 220R, HETTICH). Wavelength scan analyses of the biosensor with variable DNA targets were done using a UV–visible spectrophotometer (EVO300-PC, Thermo Scientific), while microscopic imaging was performed using a high-resolution transmission electron microscope (HRTEM, JEM 2100F, JEOL). DNA target amplification was done using a Mastercycler ep gradient S (Eppendorf, USA). The visual observation of the biosensor device through color change was captured through the use of a professional-grade full-frame digital single-lens reflex camera (D4, Nikon).
Thavanathan J., Huang N.M, & Thong K.L. (2015). Colorimetric biosensing of targeted gene sequence using dual nanoparticle platforms. International Journal of Nanomedicine, 10, 2711-2722.
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9

Synthesis of Borane-Based Compounds

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Solvents (HPLC grade) and solids (Bu4N)CN (95%), L-histidine methyl ester dihydrochloride (97%), 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (97%), 1-hydroxybenzotriazole (97%), 4-dimethylaminopyridine (99%), sodium tetraphenylborate (99%) were purchased from Sigma-Aldrich and used without additional purification. [Et3NH]2[B10H10] was prepared from decaborane-14 using the known synthetic procedure [52 (link)]. (Bu4N)[B10H11] was prepared by protonation of the [B10H10]2− anion in the CH3CN/CF3COOH system according to the method reported [53 ]. (Bu4N)[2-B10H9O(C4H8)] was synthesized from (Bu4N)[B10H11] and THF according to the known procedure [54 (link)]. The substitute was opened to give (Bu4N)2[2-B10H9OC4H8CN] when reacting with (Bu4N)CN in dichloromethane [55 (link)]; (Bu4N)2[2-B10H9OC4H8COOH] was obtained [55 (link)] by hydrolysis in boiled methanol with KOH.
Racemic rimantadine hydrochloride purchased from Zhejiang Kangyu Pharmaceutical Co (China) and hydroxychloroquine sulfate purchased from Promochem (Finland) were used as reference drugs.
Avdeeva V.V., Garaev T.M., Breslav N.V., Burtseva E.I., Grebennikova T.V., Zhdanov A.P., Zhizhin K.Y., Malinina E.A, & Kuznetsov N.T. (2022). New type of RNA virus replication inhibitor based on decahydro-closo-decaborate anion containing amino acid ester pendant group. Journal of Biological Inorganic Chemistry, 27(4-5), 421-429.
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10

Fabrication of Collagen-Chitosan Nanofiber Scaffolds

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PCL (Mw 80,000) (Sigma, New York, NY, USA) was dissolved in N-dimethylformamide and chloroform (Merck, Kenilworth, NJ, USA) by ratio 1/9 (N-dymethylformamid/chloroform). Spinning solution with concentration of 8% (w/v) was prepared. Then, the solution was electrospun upon applying a high voltage (22.5 kv) and mass flow rate of 1 ml/h at room temperature. Polymer nanofibers were collected on an aluminum foil which covered the target [1 (link)].
Collagen-chitosan film was developed by casting and solvent-evaporation method. Collagen (type I, Sigma) and chitosan (Sigma) were separately dissolved in acetic acid (0.5 M, Merck). Mixture of the 1% collagen and 1% chitosan solutions (9:1 V/V) were cast on polystyrene molds, frozen at -80℃ for 2 hours and then lyophilized in a freeze dryer for 24 hours. Scaffolds then cross-linked using 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (Sigma). The sample was rinsed in distilled water and dried at 37℃ for 4 days.
Ghanavati Z., Neisi N., Bayati V, & Makvandi M. (2015). The influence of substrate topography and biomaterial substance on skin wound healing. Anatomy & Cell Biology, 48(4), 251-257.
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