Anti runx1 aml1

Manufactured by Abcam

Anti-Runx1/AML1 is a laboratory reagent used in research applications. It is an antibody that specifically recognizes the Runx1/AML1 protein, which is a transcription factor involved in various cellular processes. This product can be used for techniques such as immunoblotting, immunoprecipitation, and immunohistochemistry to detect and study the Runx1/AML1 protein.

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Lab products found in correlation

Anti aml1, by Cell Signaling Technology (2 mentions) Supersignal west pico chemiluminescent substrate, by Thermo Fisher Scientific (2 mentions) Anti gapdh, by Cell Signaling Technology (2 mentions) Nitrocellulose membrane, by Merck Group (1 mentions) Anti bcl 2, by Bioworld Technology (1 mentions) Anti ikbα, by Abcam (1 mentions) Anti mmp9, by GeneTex (1 mentions) Bca kit, by Santa Cruz Biotechnology (1 mentions) Anti cyclin d1, by Abcam (1 mentions) Anti nf κb p65, by Abcam (1 mentions) Chemidoc mp imaging system, by Bio-Rad (1 mentions) Superenhanced chemiluminescence detection kit, by Applygen (1 mentions)

3 protocols using anti runx1 aml1

Western Blot Analysis of AML1 and GAPDH

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For Western blots, protein samples were run on a 4-20% gradient polyacrylamide gel (Biorad 456-8093) and transferred to a nitrocellulose membrane. The membrane was blocked in 5% milk in TBS for 1 hour at room temperature. The blots were hybridized with primary antibody overnight at 4 °C and washed with TBST, 4 times, 5min each. The blots were then incubated with an appropriate HRP conjugated secondary antibody (1:20,000) for 1 hour at room temperature followed by 4 washes with TBST 5 minutes each. The blot was developed using the chemiluminescent reagent (SuperSignal West Pico Chemiluminescent substrate, Thermo scientific 34080). The primary antibodies and dilutions used in this study were anti-AML1 (Cell Signalling Technology #4334, 1:1000), anti-Runx1/AML1 (Abcam ab23980, 1:3000) and anti-GAPDH (Cell Signaling Technology 14C10, 1:20,000).
Uncropped images of all blots can be found in the Supplementary Information (Supplementary Fig. 8)

Regha K., Assi S.A., Tsoulaki O., Gilmour J., Lacaud G, & Bonifer C. (2015). Developmental-stage-dependent transcriptional response to leukaemic oncogene expression. Nature Communications, 6, 7203.

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Western Blot Protein Expression Analysis

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After 48 h of transfection, protein extracts were quantified using the BCA kit (Santa Cruz Biotechnology, Santa Cruz, CA, USA) and heated for 10 min at 100°C. Total proteins (30 μg) were separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis and then transferred to nitrocellulose membranes (Merk Millipore, Germany), which was subsequently blocked by incubating with 5% (w/v) nonfat dry milk in TBST (Tris-buffered saline with Tween 20) for 1 h at room temperature. Membranes were then incubated with specific primary antibodies [anti-cyclin D1, anti-matrix metalloproteinase 2 (MMP2), anti-RUNX1/AML1, anti-NF-κB p65, anti-IKB α (Abcam, Shanghai, P.R. China), anti-Bcl-2 (BioWorld Technology, Edina, MN, USA), anti-MMP9 (GeneTex, Irvine, CA, USA), and anti-β-actin (HuaAn Biotechnology Co., Ltd., Hangzhou, P.R. China)] overnight at 4°C. Membranes were subsequently washed with TBST and incubated with horseradish peroxidase-conjugated secondary antibodies in dilution solution for 1 h at room temperature. Finally, the blots were washed and then detected using ChemiDoc™ MP Imaging System (Bio-Rad, Hercules, CA, USA) with a SuperEnhanced Chemiluminescence Detection Kit (Applygen, Beijing, P.R. China). Densitometric measurements were subsequently performed using ImageJ software (National Institutes of Health, Bethesda, MD, USA).

Wu Y., Zhang J., Zheng Y., Ma C., Liu X.E, & Sun X. (2018). miR-216a-3p Inhibits the Proliferation, Migration, and Invasion of Human Gastric Cancer Cells via Targeting RUNX1 and Activating the NF-κB Signaling Pathway. Oncology Research, 26(1), 157-171.

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Western Blot Protein Detection Protocol

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For western blots, protein samples were run on a 4–20% gradient polyacrylamide gel (Biorad 456-8093) and transferred to a nitrocellulose membrane. The membrane was blocked in 5% milk in Tris Buffered Saline (TBS) for 1 h at room temperature. The blots were hybridized with primary antibody overnight at 4 °C and washed with TBS Tween (TBST), four times, 5 min each. The blots were then incubated with an appropriate horseradish peroxidase-conjugated secondary antibody (1:20,000) for 1 h at room temperature followed by four washes with TBST 5 min each. The blot was developed using the chemiluminescent reagent (SuperSignal West Pico Chemiluminescent substrate, Thermo scientific 34080). The primary antibodies and dilutions used in this study were anti-AML1 (Cell Signalling Technology #4334, 1:1,000), anti-Runx1/AML1 (Abcam ab23980, 1:3,000) and anti-GAPDH (Cell Signaling Technology 14C10, 1:20,000).
Uncropped images of all blots can be found in the Supplementary Information (Supplementary Fig. 8)

Regha K., Assi S.A., Tsoulaki O., Gilmour J., Lacaud G, & Bonifer C. (2015). Developmental-stage-dependent transcriptional response to leukaemic oncogene expression. Nature Communications, 6, 7203.

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