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74 protocols using sigmastat 3

1

Mitochondrial CoQ6 Quantification Protocol

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CoQ6 quantification was performed using mitochondrial samples
according to previously published methods 28 (link). Densitometry analysis was carried out with a Gel Doc XR+
(Bio-Rad) with Image Lab 4.0 as software analysis. Statistical (t-Student)
analyses were carried out using the Sigmastat 3.0 (SPSS) statistical package.
Mitochondrial DNA integrity was checked in all strains using two ρ0strains, JM6 and JM8 strains. All results are expressed as the average ± SD.
Statistical analyses were carried out using the Sigmastat 3.0 (SPSS) statistical
package.
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2

Statistical Comparison of Experimental Means

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Means were compared with the Student’s t-test or one-way ANOVA with a pairwise multiple comparison procedure (Holm-Sidak method) using SigmaStat 3.0 (SPSS, Chicago, IL).
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3

Seroepidemiological Data Analysis Protocol

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The differences in seroepidemiological data among the item numbers of the groups were analysed with a χ2 test for independence. Calculating the χ2, a Yate's continuity correction was applied. Fisher's exact test was also performed. The calculations were done using GraphPadInStat V2 (GraphPad Software, V2.05a, USA). We applied the commonly used significance level, P < 0.05.
In the second part of our mathematical investigation, a factual amount of serum anti-measles IgG level was analysed. Kolmogorov–Smirnov and Shapiro–Wilk tests for normality were performed using STATISTICA Release 6.0 (StatSoft Inc., USA). In each age group, the probability distribution proved to be skew, not corresponding to the normal distribution. Because of failing normality criterion, a Kruskal–Wallis analysis of variance (ANOVA) on ranks with Dunn's post hoc multiple comparisons were used and performed with SigmaStat 3.0 (SPSS Inc., USA). The significance level of P < 0.05 has been applied, calculating with two-sided probabilities.
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4

Seasonal Variation in Male Testosterone

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Mean testosterone metabolites were calculated on a monthly basis and compared across months within each male to determine the influence of seasonality on hormone production using Kruskal–Wallis one-way analysis of variance (ANOVA) followed by Dunn's method (SigmaStat 3.0; SPSS Inc., Chicago, IL, USA). Significance was set at 95%.
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5

Statistical Analysis of Patient Data

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Data are given in mean ± standard error of the mean (SEM). For comparing patient values with control values or the patient value at the time of admission, the data was analyzed using ANOVA (analysis of variance on ranks) for nonparametric data according to Kruskal-Wallis. For analysis of significant differences over the course of time ANOVA was performed using the Student-Newman-Keuls test. For determination of specific differences Dunn's test was performed. The level of significance was set to ≤0.05. For all statistical analysis the Sigma Stat 3.0 software package (SPSS Inc., Chicago, USA) was used.
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6

Statistical Analysis of Genetic Variations

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In those cases where data did not meet the specific assumptions of normal distribution and equal variance required for parametric analysis, the Wilcoxon- Mann-Whitney Rank-Sum test was employed to compare experimental and age-matched control mice. Likewise, the one-way analysis of variance (ANOVA) and the non-parametric Kruskal-Wallis analysis of variance on ranks were used to compare data for each mouse genotype. Statistical analyses were carried out using Sigmastat 3.0 (SPSS, Inc.) and Kaleidagraph‥ Level of significance for all tests was set at p <0.05 and power was set at 0.8.
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7

Comprehensive Statistical Analysis of Data

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All data are presented as mean±SE. Statistical analysis was performed using one–way, two-way analysis of variance (ANOVA), or Student’s t-test with SigmaStat 3.0 software (SPSS, Inc., Chicago, IL), as dictated by experimental design. All the analyses were followed by Student-Newman-Keuls post hoc tests (for all pairwise comparisons) or Dunnett’s post-hoc tests (for multiple comparisons versus a control), as appropriate. Statistical significance was accepted at the 95% confidence level.
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8

Adrenal Gland mRNA Expression Analysis

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For qPCR results, the data obtained were analyzed and compared with control values (mean of normal adrenal samples) using the Mann–Whitney Rank Sum test with the SigmaStat 3.0 software package (SPSS, Chicago, IL, USA). Results of other in vitro experiments were analyzed by ANOVA followed by post hoc Tukey test. The results were considered significantly different when the P value was ≤ 0.05.
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9

ICD-10 Diagnosis and Statistical Analysis

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Data processing was based on the documented diagnosis key (according to ICD-10 coding) in the MCS ISYNET (version 6.56.1404 NFAArzt) for outpatients, or on the ICPM search list with the ORBIS Software (QRP version 1.01.02.002) and the digital patient file Pegasos for inpatients.
Statistical analysis was performed with SigmaPlot 8.0 and SigmaStat 3.0 (SPSS Inc, Chicago, USA). Firstly, a test for normal distribution was performed for each record. Depending on whether it was a normally distributed or not normally distributed sample, the specific test was performed, including analysis of variance (ANOVA). The significance level was set at P≤0.05.
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10

Spectroscopic Analysis of Metabolite Ratios

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Spectroscopy data were analyzed using the LCModel method (version 6.2-0X).22 (link) Water scaling and eddy current correction was done. We investigated the metabolites N-acetylaspartate, glutamate, and myo-inositol in ratios to creatine concentration, which all had sufficient quality, with a Cramér-Rao <20%. The Cramér-Rao is a reliability measurement and is the estimated standard deviation in percent of the estimated concentration.23 (link) Metabolite concentrations were measured as mM and ratios were calculated from metabolite concentrations. Figure 2 shows an example of the metabolite spectrum. Metabolites were fitted in the chemical shift range of 0.1–4.0 ppm.
One-way repeated measurement analysis of variance was done in SigmaStat 3.0 (SPSS Inc., Chicago, IL, USA) to test for differences in metabolite concentrations and pain intensity ratings. Pain intensity during treatment was also calculated as change compared with ratings before treatment (during treatment/before treatment ×100%). Data are presented as the mean ± standard deviation. The significance level was set at P=0.05.
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