As described in our previous study (Wang, Zhang, Moon, Hu, Wang, Martin, Sun, and Wang, 2009 (link)), the mice were placed in a contextual chamber with dimensions of 28 cm (length) × 28 cm (width) × 34 cm (height) for 2 min before receiving a mild foot shock (0.7 mA, 2 sec). Following the foot shock, the mice were left in the chamber for 1 min before returning to their home cage. The activity of the mice in the chamber was recorded by the TruScan Photo Beam Activity System (Coulbourn Instruments, Whitehall, PA). Percentage of freezing (defined as immobility) was also recorded.
Truscan photo beam activity system
Manufactured by Harvard Apparatus
Sourced in United States
The TruScan Photo Beam Activity System is a laboratory equipment device that tracks and records the movement and activity of small animals within an enclosed space. It utilizes a series of strategically placed infrared photo beams to detect and log the animals' movements and behaviors.
Automatically generated - may contain errors
Lab products found in correlation
7 protocols using truscan photo beam activity system
1
Contextual Fear Conditioning in Mice
2
Evaluating Chemotherapy Effects on Locomotor Activity
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To evaluate the effect of chemotherapy on locomotor activity, the open-field test was administered one week after the final injection. Various parameters including ambulatory movement time (sec), ambulatory moving distance (cm), center time (sec), and center distance (cm), were determined over 30 min with the ad of a TruScan Photo Beam Activity System (Coulbourn Instruments, Whitehall, PA, USA).
3
Fear Conditioning Arena Setup
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The fear-conditioning setup consisted of a 26×26×38 cm arena with a stainless steel grid floor and plexiglass walls. A dim light and a speaker that delivered white noise (55 dB) were attached to the walls and the conditioning chamber was placed into a soundproof box with a lid. Two infrared sensor rings fitted the arena and detected vertical and horizontal activity using the TruScan photobeam activity system (Coulbourn Instruments, Allentown, PA, USA)
4
Open Field Test for Murine EAE
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The OFT was performed as described previously (Wada et al., 2021 (link); Yang et al., 2010 (link)) during the early and chronic phases of EAE. In each test, a mouse was first placed in the center of a brightly-lit arena (26 cm×26 cm, 250 lux) and allowed to explore for 30 min. Mouse activity was tracked using the TruScan Photo Beam Activity System (Coulbourn Instruments, USA).
5
Open Field Locomotor Activity
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Immediately after the 2-h physical restraint, mice were placed in the center of an open field chamber (Coulbourn Instruments, Whitehall, PA) and allowed to explore freely for 30 min. The overall locomotive exploratory activity in the whole open field arena, as well as in the center area, was recorded and analyzed with the TruScan Photo Beam Activity System (Coulbourn Instruments).
6
Assessing Rodent Locomotor Activity
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To acclimate to the testing environment, the rats were transferred to the behavioral science laboratory 30 min before the experiments. Each rat was then placed in the center of the TruScan test box, and the door was closed. The rats’ spontaneous movements, distance traveled, and trajectory were recorded using the infrared detector of the TruScan Photobeam Activity System (measuring 6 × 16 × 16 in.; Coulbourn Instruments) under quiet experimental conditions. To avoid any potential influence from the scent of previously tested rats, the test box was cleaned with 10% ethanol before each trial.
Several parameters were measured during the open field test (OFT), including:
Total distance: The distance covered by the rat within the entire field.
Center distance: The number of times the rat entered the central area of the open field.
Vertical plane (VP) time: The total time the rat spent in the VP, which refers to rearing behavior.
VP entries: The total number of times the rat entered the VP.
All data were automatically calculated and recorded for each rat using the same video tracking system.
Several parameters were measured during the open field test (OFT), including:
Total distance: The distance covered by the rat within the entire field.
Center distance: The number of times the rat entered the central area of the open field.
Vertical plane (VP) time: The total time the rat spent in the VP, which refers to rearing behavior.
VP entries: The total number of times the rat entered the VP.
All data were automatically calculated and recorded for each rat using the same video tracking system.
7
Evaluating Mice Locomotor Activity
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To evaluate the activity of the mice, an open field test was performed as per previous studies' protocols [32, 36] . For each test, a mouse was first placed in the center of an open field, and various parameters, including the ambulatory move time (s) and ambulatory distance (cm), were determined over a 30-min period using a TruScan Photo Beam Activity System (Coulbourn Instruments, Whitehall, PA, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!