Scanasyst

Manufactured by Veeco

Sourced in United States

ScanAsyst is a versatile lab equipment product designed for advanced imaging and measurement applications. It provides high-resolution, non-contact scanning capabilities to support various research and analytical needs. The core function of ScanAsyst is to enable precise, high-quality data acquisition without the need for extensive user intervention or complex setup procedures.

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Lab products found in correlation

Mica surface, by Ted Pella (1 mentions) Multimode 8 afm, by Veeco (1 mentions) Poly ornithine, by Merck Group (1 mentions) Dimension icon, by Veeco (1 mentions)

5 protocols using scanasyst

AFM Imaging of DNA-Binding on Mica

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For AFM imaging, the sample (15 μl) was deposited onto a freshly cleaved mica surface (Ted Pella, Inc.) and left to adsorb for 1 min. A volume of 40 μl of 1× TAE-Mg²⁺ and 2 to 15 μl 100 mM NiCl₂ was added onto the mica, and the sample was scanned on a Veeco 5 Multimode AFM in the Scanasyst in Fluid mode using Scanasyst in fluid+ tips (Veeco, Inc.).

Han D., Qi X., Myhrvold C., Wang B., Dai M., Jiang S., Bates M., Liu Y., An B., Zhang F., Yan H, & Yin P. (2017). Single-stranded DNA and RNA origami. Science (New York, N.Y.), 358(6369), eaao2648.

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Biological Sample Imaging with AFM

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A BioScope Catalyst AFM with ScanAsyst
(Veeco, Camarillo, CA, USA) was used for imaging. Deflection images
were taken over various surface areas. The scans were made in contact
mode under the lowest possible applied force (1 to 2 nN) at a scan
rate of 1 Hz using DNP probes from Veeco (Woodbury, USA).

Dong J.J., Muszanska A., Xiang F., Falkenberg R., van de Belt-Gritter B, & Loontjens T. (2019). Contact Killing of Gram-Positive and Gram-Negative Bacteria on PDMS Provided with Immobilized Hyperbranched Antibacterial Coatings. Langmuir, 35(43), 14108-14116.

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AFM Imaging of ds RNA-DNA Hybrids

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For AFM imaging of the ds RNA–DNA hybrid structures, 30 μl of 0.1 mg ml⁻¹ polyornithine (Sigma) solution was added to freshly cleaved mica and stand for 3 min to increase the binding to the structures before applying the samples. Then the mica was rinsed with 1 ml water and dried with compressed air. An aliquot of 5 μl of each sample (about 5 nM) in 1 × TAE-Mg buffer (11 mM MgCl₂, 40 mM Tris, 20 mM acetic acid, 1 mM EDTA, pH 8.0) was applied to the treated mica and stand for 1 min. Then the mica was rinsed with 1 ml water and dried with compressed air. AFM imaging was performed on a Veeco MultiMode 8 AFM in the ScanAsyst in air mode using the scanayst-air tips (Veeco). The AFM images were processed with the software Gwyddion.

Liu D., Shao Y., Chen G., Tse-Dinh Y.C., Piccirilli J.A, & Weizmann Y. (2017). Synthesizing topological structures containing RNA. Nature Communications, 8, 14936.

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Characterizing Surface Wettability and Topography

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Water contact angles in air were measured on PDMS-coated slides and PAA-coated PDMS slides before and after oxidation by the sessile drop technique (4 µL of water) using a goniometer and analyzed with ImageJ. Data are expressed as a mean ± standard deviation (n = 3). Surface topography of PAA-coated PDMS slides oxidized through patterns was measured by atomic force microscope in ScanAsyst mode (Veeco, Bruker) and analyzed with Nanoscope (Veeco, Bruker).

Kojima T., Moraes C., Cavnar S.P., Luker G.D, & Takayama S. (2014). Surface-templated hydrogel patterns prompt matrix-dependent migration of breast cancer cells towards chemokine-secreting cells. Acta biomaterialia, 13, 68-77.

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Tapping Mode AFM Imaging Protocol

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The AFM images were recorded using a Bruker Dimension Icon with ScanAsyst® (Veeco Instruments Inc., San Jose, USA). All the AFM images were recorded in the tapping mode.

Ngongang Ndjawa G.O., Tchalala M.R., Shekhah O., Khan J.I., Mansour A.E., Czaban-Jóźwiak J., Weselinski L.J., Ahsaine H.A., Amassian A, & Eddaoudi M. (2019). The Growth of Photoactive Porphyrin-Based MOF Thin Films Using the Liquid-Phase Epitaxy Approach and Their Optoelectronic Properties. Materials, 12(15), 2457.

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