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R1881

Manufactured by Selleck Chemicals
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R1881 is a synthetic androgen receptor agonist. It is a laboratory chemical used for research purposes.

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Lab products found in correlation

Enzalutamide, by Selleck Chemicals (2 mentions) Mdv3100, by Selleck Chemicals (1 mentions) Fetal bovine serum (fbs), by Gemini Bio (1 mentions) Dulbecco modified eagle medium (dmem), by Corning (1 mentions) Synergy 2 plate reader, by Agilent Technologies (1 mentions) Nutlin 3, by Selleck Chemicals (1 mentions) Arn 509, by Selleck Chemicals (1 mentions) Dihydrotestosterone dht, by Selleck Chemicals (1 mentions) Mg132, by Selleck Chemicals (1 mentions)

4 protocols using r1881

1

Androgen Receptor Modulation Assay

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Assay was performed as previously described (22 ). Briefly, shRNA or pLKO control virus was added to fibronectin-coated (1μg/ml) 96well plates. Cells were plated in RPMI-1640 plus 5%CSS with vehicle, 0-0.05nM R1881, and/or 10μM MDV3100 (Selleck). Quantification was performed on Day 7 using a BioTek Synergy 2 plate reader.
Ta H.Q., Ivey M.L., Frierson HF J.r., Conaway M.R., Dziegielewski J., Larner J.M, & Gioeli D. (2015). Checkpoint kinase 2 (CHK2) negatively regulates androgen sensitivity and prostate cancer cell growth. Cancer research, 75(23), 5093-5105.
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2

Prostate Cancer Cell Culture Conditions

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BMPC1, BMPC2, and MYC-CaP cells were routinely cultured in Dulbecco modified Eagle medium (DMEM) (Corning Inc., Corning, NY) supplemented with 10% Fetal Bovine Serum (FBS) (Gemini Bio-Products, Sacramento, CA) at 37°C in 5% CO2. PC3 cells were cultured under similar conditions in RPMI media. Ten percent Charcoal-stripped Calf Serum (CCS) (Gemini Bio-Products) supplemented media was used in select experiments. Enzalutamide and R1881 were purchased via Selleck Chemicals (Houston, TX) and Sigma-Aldrich (St. Louis, MO), respectively.
Markowski M.C., Hubbard G.K., Hicks J.L., Zheng Q., King A., Esopi D., Rege A., Yegnasubramanian S., Bieberich C.J, & De Marzo A.M. (2018). Characterization of novel cell lines derived from a MYC-driven murine model of lethal metastatic adenocarcinoma of the prostate. The Prostate, 78(13), 992-1000.
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3

Enzalutamide Modulation of R1881 Effects

Cited 1 time
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Assay was performed as previously described [18 (link)]. Briefly, shRNA or pLKO control virus was added to 1 μg/ml fibronectin-coated 96well plates. Cells were plated in RPMI-1640 plus 5%CSS with vehicle, 0–0.05 nM R1881, and/or 10 μM Enzalutamide (Selleck Chemicals). Quantification was performed on Day 7 using a BioTek Synergy 2 plate reader.
Ta H.Q., Whitworth H., Yin Y., Conaway M., Frierson HF J.r., Campbell M.J., Raj G.V, & Gioeli D. (2019). Discovery of a novel long noncoding RNA overlapping the LCK gene that regulates prostate cancer cell growth. Molecular Cancer, 18, 113.
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4

Prostate Cancer Cell Culture and Inhibitor Assays

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LNCaP, PC3 and 22RV1 cells (Chinese Academy of Sciences) were cultured using RPMI1640 containing 10% serum. Myc-CaP cells were obtained from Shanghai Jiao Tong University (Shanghai, China). HEK293T was provided by the Stem Cell Bank and cultured using DMEM containing 10% serum. Androgen deprivation was conducted by culturing cells in RPMI1640 (5% charcoal-stripped serum) for 48 h.
ENZ (Cat# HY-70002), actinomycin D (ACTD) (Cat# HY-17559) and cycloheximide (CHX Cat# 66-81-9) were purchased from MCE. ARN-509 (Cat# 956104-40-8), ODM-201 (Cat# 1297538-32-9), MG132 (Cat# 1211877-36-9), dihydrotestosterone (DHT, Cat# 521-18-6), Nutlin3 (Cat# 890090-75-2) and R1881(Cat# 965-93-5) were obtained from Selleck. Recombinant Human MDM2/HDM2 Protein was obtained from R&D.
Li Y., Chu Y., Shi G., Wang X., Ye W., Shan C., Wang D., Zhang D., He W., Jiang J., Ma S., Han Y., Zhao Z., Du S., Chen Z., Li Z., Yang Y., Wang C., Xu X, & Wu H. (2022). A novel inhibitor of ARfl and ARv7 induces protein degradation to overcome enzalutamide resistance in advanced prostate cancer. Acta Pharmaceutica Sinica. B, 12(11), 4165-4179.
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