A field emission scanning electron microscope (FE-SEM; JSM-7500F; JEOL, Tokyo, Japan), and an atomic force microscope (AFM; Nanoscope IIIa, Digital Instruments, Tonawanda, NY, USA) with a J scanner were used to assess the morphology of cystamine-conjugated GO and the cells. An Escalab MK II photoelectron spectrometer (VG Scientific Ltd., East Sussex, UK) was used for X-ray photoelectron spectroscopy (XPS) measurements. A Varian ultraviolet-visible spectrophotometer was used for measuring absorbance. A Varian 3100 Fourier transform infrared (FT-IR) (Excalibur series) spectrophotometer was used for FT-IR spectra measurements. A ZetaSizer (Nano-Z; Malvern Instruments, Malvern, UK) was used for zeta potential measurement.
Ultraviolet visible spectrophotometer
Manufactured by Agilent Technologies
Sourced in United States, Australia
The Ultraviolet-visible spectrophotometer is a laboratory instrument used to measure the absorption or transmittance of light in the ultraviolet and visible regions of the electromagnetic spectrum. It is a core tool for quantitative analysis and identification of various chemical compounds.
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3 protocols using ultraviolet visible spectrophotometer
1
Multifaceted Characterization of Cystamine-Conjugated Graphene Oxide
2
Starch Content Quantification in Rice
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A total of 5.00 g fresh instant rice was put into a 50 mL colorimetric tube. Water was added to 25 mL and the mixture was shaked at 40°C for 1 h, followed by the dilution to 50 mL. After centrifuged at 3,000 rpm for 15 min, 0.5 mL KI-I2 solution (0.1 mol/L) and 0.5 mL HCl (0.1 mol/L) were added to 5 mL of supernatant, followed by settling to permit. After 15 min standing, iodine color value was measured at the wavelength of 620 nm (18 , 19 (link)) (CARY100; Ultraviolet-Visible Spectrophotometer, Varian, USA).
3
Spectral Characterization of AC-Cobalt Complex
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In brief, 1 mL of 30 mg/L AC was mixed with 1 mL of 25 g/L cobalt acetate tetrahydrate aqueous solution and allowed to stand for 30 min in the dark. Afterward, the mixture was diluted separately with 8 mL of citrate-phosphate buffer solution (0.1 M citric acid monohydrate+ 0.2 M disodium hydrogen phosphate) at different pH values of 3.5, 4.5, 5.5, and 6.5. Changes in spectral properties [lambda max (λmax) and relative absorbance] were recorded for each pH (for AC and AC-cobalt complex) using a ultraviolet-visible spectrophotometer (Varian Medical Systems Australasia, Belrose, NSW, Australia).
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