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9 protocols using potassium permanganate

1

Synthesis of Transition Metal Sulfates

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Zinc sulfate (ZnSO4·H2O, 99.9%), sodium sulfate (Na2SO4, 99.9%), and manganese sulfate (MnSO4·H2O, 99.9%) were purchased from Aladdin (China). Sodium silicate (Na2SiO3·9H2O) was obtained from Xilong Scientific Co., Ltd (China). Zn foil (99.98%) and titanium foil (99.8%) were provided by Alfar Aesar Chemicals (China). Manganese chloride tetrahydrate (MnCl2·4H2O, 99%), sodium hydroxide (NaOH), and potassium permanganate (KMnO4) were supplied by Beijing Chemical Factory (China). All the chemicals were used as received.
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2

Preparation of Amyloid-beta Peptide

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1–42 peptide was synthesized by Science Peptide Biological Technology Co., Ltd. (Shanghai, China). Graphite powder (size less than 30 µm) and 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP) were purchased from Sinopharm Chemical Reagent Co., Ltd. Concentrated sulfuric acid, sodium nitrate, potassium permanganate, hydrochloric acid, hydrogen peroxide, sodium hydroxide and sodium carbonate were purchased from Beijing Chemical Plant. MTT (3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide) was bought from Solarbio Science & Technology Co., Ltd. (Beijing, China). Deionized water used in all experiments was obtained from Milli-Q Integral 3 (Merck Millipore, France).
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3

Synthesis of Magnetic Nanocomposites

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Sulfuric acid (H2SO4, 99%; Beijing Chemical Works, China),
hydrochloric acid (HCl, ≥37%; Beijing Chemical Works, China), potassium
permanganate (KMnO4, ≥99%; Beijing Chemical Works, China), hydrogen
peroxide (H2O2, 30%; Beijing Chemical Works, China),
sodium nitrate (NaNO3, ≥99%; Sinopharm Chemical Reagent Co. Ltd.,
China), citric acid monohydrate
(C6H8O7·H2O, ≥99.5%; Sinopharm
Chemical Reagent Co. Ltd., China), natural powder graphite (325 mesh, 99.99%;
Alfa Aesar) and magnetite (Fe3O4; Xiya Reagent) were
acquired. All reagents were analytical grade and used without purification.
Water was purified using ultrapure water equipment.
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4

Synthesis of Graphene Oxide Magnetic Nanocomposites

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Graphite (> 100 mesh, 99.95%) and ammonium persulphate ((NH4)2S2O8, 99%) were both purchased from Shanghai Aladdin Bio-Chem Technology Co., Ltd., China. Ferroferric oxide (Fe3O4, 99.5%) was provided by Shanghai Macklin Biochemical Co., Ltd., China. Pyromellitic dianhydride (PMDA, 99%) and 4, 4′-oxydianiline (ODA, 99%) were both supplied by Beijing J&K Scientific Co., Ltd., China. Dimethylacetamide (DMAc, 99%) was purchased from Guangdong Guanghua Technique Co., Ltd., China. Concentrated sulfuric acid (H2SO4, 95–98%), potassium permanganate (KMnO4) and hydrogen peroxide (H2O2, 30%) were all supplied by Beijing Chemical Works, China. All materials were used without further purification.
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5

Flame-Retardant Polymer Composite Preparation

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Polyether polyol (type:1012a) and isocyanate (type:1012b) were purchased from Shenzhen Keshengda Trading Co., Ltd. (Shenzhen, China). Concentrated sulfuric acid (98%, AR), potassium permanganate (AR), and sodium nitrate (AR) were supplied by the Beijing Chemical Factory (Beijing, China). Hydrogen peroxide (30%, AR) and anhydrous ethanol (AR) were bought from the Tianjin Yongda Chemical Reagent Co., Ltd. (Tianjin, China). Phytic acid (PA) and the silane coupling agent KH550 (GR) was bought from the National Pharmaceutical Group Chemical Reagent Co., Ltd. (Shanghai, China). Ammonium polyphosphate (APP) was purchased from the Tangshan Yongfa Flame Retardant Material Factory (Tangshan, China). Graphite powder was bought from the Tianjin Zhiyuan Chemical Reagent Co., Ltd. (Tianjin, China). Expandable graphite (EG, type: ADT150) was purchased from the Shijiazhuang Kepeng Flame-retardant Material Factory (Shijiazhuang, China).
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6

Graphene-Based Electrochemical Biosensor

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Graphite powder (99.95%, Shanghai Aladdin Reagent Co., Ltd., Shanghai, China); hydrochloric acid, sulfuric acid, sodium nitrate, and potassium permanganate (30%, 98%, 99%, 99%, Beijing Chemical Plant, Beijing, China); potassium ferricyanide, urea, ethanol, DA, and tetrachloroauric(III) acid trihydrate (99.5%, 99%, 99.5%, 98%, 99.9%, Beijing Innochem Technology Co., Ltd.,Beijing, China); potassium chloride and uric acid (99.5%, 99%, Beijing Oka Biotechnology Co., Ltd., Beijing, China); aptamers (Shanghai Sangon Biotech Bioengineering Co., Ltd., Shanghai, China); and 0.1 M phosphate buffered solution (PBS), which was self-made, were all used in this study.
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7

Graphite-Based Polymer Composite Synthesis

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Graphite (325 mesh) powder was purchased from Qingdao Xinghe Graphite Co. Ltd. h-BN (diameter = ∼1 μm, purity > 98%) powder and calcium chloride were both purchased from Sigma Aldrich Corporation. PEO (Mw = ∼100 000) was purchased from Alfa-Aesar Corporation. Also, 98% sulfuric acid, 37% hydrochloric acid, 30% hydrogen peroxide, potassium permanganate, sodium nitrate, and hydrazine hydrate were all purchased from Beijing Chemical Works. All chemicals were used as received.
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8

Graphite Flakes Production Protocol

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Natural graphite flakes were obtained from Huadong Graphite Factory (China) with an average diameter of 13 μm. Potassium permanganate (KMnO4, 99%), concentrated sulfuric acid (H2SO4, 96%), hydrochloric acid (HCl, 36%), sodium nitrate (NaNO3, 98%), ammonia (25% to 38%), ethanol (99.7%) were all obtained from Beijing Chemical Factory (China) and used as received. PPD was obtained from Tianjin Guangfu Fine Chemical Research Institute Research Institute (China).
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9

Graphene Oxide Composite Synthesis

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Concentrated sulfuric acid (H 2 SO 4 , 98%), nitric acid (HNO 3 , 70%), sodium nitrate (NaNO 3 ), potassium permanganate (KMnO 4 ), hydrogen peroxide (H 2 O 2 , 38%), hydrochloric acid (HCl, 38%), ethanol, and ascorbic acid were purchased from Beijing Chemical Factory (China). Pristine graphite was supplied by Huadong Graphite Factory (China). Epoxy resin (JY-257) with an epoxy value of 0.53-0.55 mol/100g was obtained from Jiafa Chemical Co. (China). Methyl hexahydrophthalic anhydride as the curing agent and tris-(dimethyl aminomethyl) phenol as the curing accelerator were purchased from Alfa Aesar (China).
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