Nanoscan pet ct scanner

C57BL/6 mice (6–8 weeks) were i.v. injected with MC38 cells (1 × 10⁵). On day10, mice were randomly divided into groups (6–7 per group) that were respectively vaccinated with (1) PBS, (2) CpG and Adpgk, and (3) iDR-NCs and Adpgk, by subcutaneous injection in 50 μL at the base of tail on day10, day 16, and day 22 post tumor inoculation. Tumor burden was quantified at the end of study using FDG radiotracer. Specifically, mice were anesthetized for 30 min using isoflurane/O² (2% v/v) before injection. Anesthetized mice were injected i.p. with FDG (3.7 MBq per mouse) in PBS (100 μL). Mice continued to be anesthetized for 1 h, and then, one mice from each group was randomly picked to be scanned for PET/CT on a nanoScan PET/CT scanner (Mediso Medical Imaging Systems). Meanwhile, mice were euthanized and organs of interest were resected, followed by measuring ¹⁸F radioactivity on a gamma-counter (Wallac Wizard 1480, PerkinElmer). The radioactivity in organs was converted to calculate the percentages of the %ID and %ID per g in organs of interest. Results were analyzed using GraphPad Prism 4 (La Jolla, CA).

PET imaging was performed on a dedicated small animal nanoScan PET/CT scanner (Mediso Ltd., Hungary). Four mice from each of the groups that received 110 µg of either [⁸⁹Zr]Zr-CX-2009, [⁸⁹Zr]Zr-CX-191, [⁸⁹Zr]Zr-CX-1031, or [⁸⁹Zr]Zr-CX-090 were imaged at 24 and 72 h p.i., with additional imaging at 168 h p.i. for [⁸⁹Zr]Zr-CX-2009. Mice were anesthetized by inhalation of 2% to 4% isoflurane/O₂ during the entire scanning period (1 h duration per time point). A 5 min computed tomography (CT) scan was acquired prior to each PET scan and was used for attenuation and scatter correction purposes. Reconstruction was performed by three-dimensional reconstruction (Tera-Tomo; Mediso Ltd.) with four iterations and six subsets, resulting in an isotropic 0.4 mm voxel dimension. The scanner was cross-calibrated with the dose calibrator and well counter, enabling accurate measurement of standardized uptake values (SUVs). SUVs were calculated as the ratio of the radioactivity activity concentration (kBq/mL) as measured by the PET scanner within the region of interest (ROI), divided by the decay-corrected amount of injected radiolabeled compound corrected for the weight of the animal. The software Amide (GNU General Public License, Version 2, Made.exe 0.9.2) was used to draw and quantify the ROIs, and VivoQuant was used to capture the images that are displayed.

At 48 hours or 11 days after stroke, mice (n = 15) were injected with
2-deoxy-2-[¹⁸F]fluoro-D-glucose (302.4 ± 16.4 µCi) in
phosphate-buffered saline (PBS) through a tail vein catheter. The animals were
kept under isoflurane anaesthesia (4% for induction, 1.5–2% (in O₂)
for maintenance) and the radiotracer was allowed to circulate for 60 minutes
prior to a static PET/CT scan with a nanoScan PET/CT scanner (Mediso, Hungary).
A 3-min whole-body CT scan (energy 50 kVp, current 180 µAs, isotropic voxel size
0.25 mm³) was followed by a 20- or 30-min static PET scan.
Coincidences were filtered with an energy window between 400 and 600 keV.
Reconstruction was performed with four full iterations, six subsets per
iteration with an isotropic voxel size of 0.4 mm³ using the TeraTomo
3D reconstruction algorithm (Mediso Nucline nanoScan v 3.00.020.0000). CT-based
attenuation correction was applied for PET reconstruction. Animals were
euthanized with an overdose of isoflurane immediately after the PET/CT scan.

Twenty‐four plaque‐bearing rabbits were randomly assigned to the control, LIFU, PFP–HMME@PLGA/MnFe₂O₄–Ram, PFP@PLGA/MnFe₂O₄–Ram + LIFU, PFP–HMME@PLGA/MnFe₂O₄ + LIFU, and PFP–HMME@PLGA/MnFe₂O₄–Ram + LIFU groups (n = 4 each). On day 28 after treatment, the rabbits were fasted overnight. Subsequently, ¹⁸FDG (0.1 mCi kg⁻¹) was injected intravenously 1 h before the right femoral arteries containing advanced plaques were harvested and cut into two segments. ¹⁸FDG‐PET/CT imaging of the femoral arteries was performed using a nanoScan PET/CT scanner (Mediso, USA). For PET/CT imaging in 3D mode, the arteries were covered for 20 min in a single bed position, and the total standardized uptake value (SUV) was measured using the PMOD software.

Imaging experiments were performed using a nano-Scan^® PET/CT scanner (Mediso Medical Imaging Systems, Budapest, Hungary). ⁶⁴Cu-GTSM-PET was performed in 6- and 9-week-old female Npc1^−/− mice and age-matched WT controls (n = 7 in 9-week-old Npc1^−/− group, n = 6 in 6-week-old Npc1^−/− group, n = 5 in WT groups). All animals were anaesthetised by isoflurane inhalation (3%, Vet Tech Solutions Ltd.) for immobilisation and injected with ⁶⁴Cu-GTSM (~ 15 to 20 MBq, ≤ 200 µL) via a lateral tail vein. Immediately after injection, mice were placed on the scan bed in the prone position and imaged for 30 min by PET/CT (PET: 400-600 keV energy window, 5 ns coincidence window, 0.30 × 0.30 × 0.30 mm³ voxel size; CT: 180 projections, 45 KVp, 0.25 × 0.25 × 0.21 mm³ voxel size). Anaesthesia was maintained with 1.5-2% isoflurane throughout the duration of the scan. Respiration rate and bed temperature were monitored during image acquisition. After imaging, mice were allowed to recover and housed overnight. At 15 h post-injection of ⁶⁴Cu-GTSM, animals were re-scanned as described above.

CT scans were acquired on a Nanoscan PET/CT scanner from Mediso using Nucline v2.01 software. All mice were kept sedated under isoflurane anesthesia for the duration of the scan. Scans were acquired with an X-ray tube energy and current of 70 kVp and 280 uA, respectively. 720 projections were acquired per rotation, for three rotations, with a scan time of approximately 11 min, followed by reconstruction with a RamLak filter and voxel size 40 × 40 × 122 µm. For ex vivo analyses, mouse heads were fixed in 10% formalin buffered saline, followed by scanning and reconstruction with 1440 projections per revolution. Cranial volume was measured using VivoQuant software (v2.50patch2) using the spline tool to manually and accurately draw around the circumference of the cranium on multiple stepwise 2D slices.

Preclinical PET/CT
images were acquired using a NanoScan PET/CT scanner (Mediso, Budapest,
Hungary) with mice under 0.8–1.5% isoflurane in oxygen anesthesia
and warmed to 37 °C for the duration of the experiment. Mice
were administered radiolabeled Fab fragments (∼50 μg,
∼ 2.1–3.6 MBq) in 200 μL of PBS via intravenous
tail injection. Dynamic PET scans were acquired for up to 4 h post
injection, followed by a CT scan for anatomical visualization (480
projections; helical acquisition; 55 kVp; 600 ms exposure time). PET/CT
data sets were reconstructed using a Monte Carlo-based full-3D iterative
algorithm (TeraTomo, Mediso) with 4 iterations, 6 subsets, and 0.4
mm isotropic voxel size. Images were coregistered and analyzed using
VivoQuant v.3.0 (Invicro). Regions of interest (ROIs) were delineated
for the PET activity quantification in specific organs. Uptake in
each ROI was expressed as a percentage of injected dose per gram of
tissue (% ID/g).