For hematoxylin and eosin (H and E) staining, epididymal adipose tissues were fixed in 10% neutral-buffered formalin (Thermo Fisher Scientific, Waltham, MA, USA) overnight and transferred to 70% ethanol for one day. Afterwards, the tissues were processed in a routine manner for paraffin sections (Tissue Tek VIP Tissue Processor; Sakura Finetek USA, Torrance, CA, USA). Paraffin-embedded sections (5 μm) were cut and stained with H and E (Sigma-Aldrich) for microscopic examination (Olympus BX60, Waltham, MA, USA) at 20× magnification. To quantitate adipocyte size, the H and E-stained sections were analyzed using the ImageJ software (National Institutes of Health, Bethesda, MD, USA).
Tissue tek vip tissue processor
Manufactured by Sakura Finetek
Sourced in United States
The Tissue Tek VIP Tissue Processor is a laboratory equipment designed for the automated processing of tissue samples. It is used to prepare tissue specimens for histological analysis by dehydrating, clearing, and infiltrating the samples with paraffin wax. The Tissue Tek VIP Tissue Processor efficiently processes multiple tissue samples simultaneously, ensuring consistent and reliable results.
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Lab products found in correlation
2 protocols using tissue tek vip tissue processor
1
Quantitative Adipocyte Morphometry via H&E Staining
2
Quantifying Mesenteric Adipocyte Morphology
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At the times of tissue collection, the mesenteric white adipose tissues were stored at −80 °C until needed. For hematoxylin and eosin (H&E) staining, a portion of the mesenteric adipose tissues were fixed in chilled 10% neutral-buffered formalin (Thermo Fisher Scientific, Waltham, MA) overnight at room temperature and transferred to 70% ethanol. Afterwards, the tissues were processed in a routine manner for paraffin sections (Tissue Tek VIP Tissue Processor; Sakura Finetek USA, Torrance, CA) and embedded the following morning. Paraffin-embedded sections (5 μm) were cut and stained with H&E (Sigma-Aldrich) for microscopic examination (Olympus BX60, Waltham, MA) at 20× magnification. To quantitate adipocyte size, the H&E-stained sections were analyzed using the ImageJ software (National Institutes of Health, Bethesda, MD). For each animal, four fields were taken from one section and the area of 10 adipocytes were measured. The images were randomized and blinded with a numerical ID.
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