Human brain sections from two different MS patients (female RRMS 49 y.o. and female 33 y.o. RRMS) were stained following established protocols (12 (link), 30 (link), 31 (link)). After fixation with acetone and ethanol 70% at -20°C and blocking with 10% mixed donkey/goat serum, sections were incubated for 1 h at 20°C with primary antibodies diluted in 3% donkey serum: mouse anti-human granzyme-B (1/20, R&D Systems, Cat#MAB2906) and rabbit anti-human NogoA (1/100, EndMillipore, Cat# AB5664P). Adequate secondary antibodies for these antibodies were used: donkey rabbit RRX (1:500, Jackson ImmunoResearch, Cat#711-295-152) and goat anti-mouse AlexaFluor635 (1:250, Invitrogen, Cat#A31574). Then, sections were blocked again in 10% rabbit serum and stained using mouse anti-human CD4-FITC antibody (1:50, BD Biosciences, Cat#555346), followed by a secondary antibody rabbit anti-FITC (1:50, Invitrogen, Cat#A11090). Finally, nuclei were stained using DAPI. Sections were mounted on slides with Prolong Gold Antifade.
Mouse anti human cd4 fitc antibody
Manufactured by BD
Mouse anti-human CD4-FITC antibody is a fluorescently labeled monoclonal antibody that binds to the CD4 surface antigen expressed on a subset of human T lymphocytes.
Automatically generated - may contain errors
Lab products found in correlation
Mouse anti human foxp3 pe, by Thermo Fisher Scientific (1 mentions)
Pe cyanine7, by Thermo Fisher Scientific (1 mentions)
Fluorescein isothiocyanate (fitc), by Thermo Fisher Scientific (1 mentions)
Facscanto 2 flow cytometer, by BD (1 mentions)
Red blood cell lysis buffer, by Solarbio (1 mentions)
Live dead fixable aqua dead cell stain, by Thermo Fisher Scientific (1 mentions)
Lsrfortessa cell analyzer, by BD (1 mentions)
Klrg1, by BD (1 mentions)
Facs fixing buffer, by BD (1 mentions)
Ctla 4, by BD (1 mentions)
3 protocols using mouse anti human cd4 fitc antibody
1
Characterizing Immune Cells in Multiple Sclerosis Brains
2
Multiparameter Flow Cytometry Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Mouse anti-human CD3-PE-Cyanine7 antibody (eBioscience, catalog number: 25-0038-42), mouse anti-human CD4-FITC antibody (BD Pharmingen, catalog number: 340133), mouse anti-human CD8-PE (BD Pharmingen, catalog number: 340046), mouse anti-human CD25-APC antibody (Biolegend, catalog number: 302610), and mouse anti-human Foxp3-PE (eBioscience, catalog number: 12-4777-42) Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE (eBioscience, catalog number: 12-4714-42), Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), FITC (eBioscience, catalog number: 11-4714-82), Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), APC (eBioscience, catalog number: 17-4714-42) Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-Cyanine7 (eBioscience, catalog number: 25-4714-80) were used. Peripheral blood, livers, spleens, lungs and guts were collected at the time of necropsy and analyzed by flow cytometry. Single cell 1×106/mL suspensions were obtained by grinding the liver, spleen, lung, and gut, and blood samples were processed with red blood cell lysis buffer (Solarbio, catalog number: R1010) at 4°C according to the protocol. All samples were stained with antibodies or isotype matched control IgG for 30 min at 4°C in the dark and analyzed with a BD FACS CantoII flow cytometer with FlowJo 10.0.
3
Comprehensive Immune Phenotyping of PBMCs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
0.6 × 106 PBMCs were washed with phosphate-buffered saline (PBS) (Sigma) and first stained with LIVE/DEAD™ Fixable Aqua Dead Cell Stain (Life Technologies) for 20 min, followed by surface staining with mouse anti-human CD3-AF700 antibody, mouse anti-human CD8-APC-H7 antibody, mouse anti-human CD4-FITC antibody, mouse anti-human TIGIT-PE-A antibody, mouse anti-human TIM-3-BB515 antibody and 2B4-APC antibody, CTLA4, PD-1, and KLRG1 (BD) for 20 min. Finally, 300 µL FACS fixing buffer (BD) was added to fix the cells for acquisition by flow cytometry. All data were acquired on BD LSRFortessa™ Cell Analyzer (BD).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!