(TEOS), CytC (from equine heart), 2, 2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic
acid) diammonium salt (ABTS), and Coomassie Brilliant Blue G-250 were
purchased from Sigma-Aldrich. Phosphoric acid and hydrogen peroxide
(30% solution) were purchased from EMD Millipore. HRP was purchased
from Gold Biotechnology. Guaiacol was purchased from Cayman Chemical
Company. Premium grade glass coverslips (25 mm × 25 mm ×
1 mm) were purchased from Fisher Scientific. All chemicals and materials
were used as received, with the exception of the glass coverslips,
which were cleaned prior to use. All UV–vis spectra were obtained
via a Shimadzu UV-2101PC UV–vis spectrometer.