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Hsp90 835 16f1

Manufactured by Enzo Life Sciences
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Hsp90-835-16F1 is a laboratory equipment product manufactured by Enzo Life Sciences. It is designed to facilitate research and analysis related to heat shock protein 90 (Hsp90). The core function of this product is to provide a tool for researchers to study the structure, function, and interactions of Hsp90 in various biological systems.

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Lab products found in correlation

C src, by Cell Signaling Technology (2 mentions) Flag tag (flag), by Thermo Fisher Scientific (2 mentions) Hsp90α, by Cell Signaling Technology (2 mentions) Fnip2, by Cell Signaling Technology (2 mentions) V src, by Merck Group (2 mentions) Anti ha antibody conjugated agarose beads, by Merck Group (2 mentions) Anti flag, by Merck Group (2 mentions) Gapdh, by Cell Signaling Technology (2 mentions) Protease inhibitor cocktail, by Roche (2 mentions) Phosstop, by Roche (2 mentions)

Close spelling variants of this product

HSP90 (23 citations)

2 protocols using hsp90 835 16f1

1

Immunoprecipitation of Protein Complexes

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Protein extraction from both yeast and mammalian cells was carried out using methods previously described (Mollapour et al., 2010 (link)). For immunoprecipitation, mammalian cell lysates were incubated with anti-FLAG or anti-HA antibody conjugated agarose beads (Sigma) for 2 h at 4°C. Immunopellets were washed 4 times with fresh lysis buffer (20mM Tris-HCl (pH 7.4), 100 mM NaCl, 1 mM MgCl2, 0.1% NP40, protease inhibitor cocktail (Roche), and PhosSTOP (Roche)) and eluted in 5x Laemmli buffer. Precipitated proteins were separated by SDS-PAGE and transferred to nitrocellulose membranes. Co-immunoprecipitated proteins were detected by immunoblotting with antibodies recognizing FLAG, 6x-His (ThermoFisher Scientific), Hsp90-835-16F1, GAPDH, p23 (ENZO Life Sciences), Tsc1, FLCN, GR, Myc, V5, GAPDH, Hsp90α, FNIP2, c-Src (Cell Signalling), phospho-tyrosine, v-Src (Millipore), FNIP1, FNIP2 (NCI), FNIP1 (antibodies-online.com), Aha1 (StressMarq Biosciences), HA (Roche). Secondary antibodies raised against mouse, rabbit, and rat (Cell Signaling) and goat (Santa Cruz Biotechnology) were used (See Key resources table).
Backe S.J., Sager R.A., Regan B.R., Sit J., Major L.A., Bratslavsky G., Woodford M.R., Bourboulia D, & Mollapour M. (2022). A specialized Hsp90 co-chaperone network regulates steroid hormone receptor response to ligand. Cell reports, 40(2), 111039.
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2

Immunoprecipitation of Protein Complexes

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Protein extraction from both yeast and mammalian cells was carried out using methods previously described (Mollapour et al., 2010 (link)). For immunoprecipitation, mammalian cell lysates were incubated with anti-FLAG or anti-HA antibody conjugated agarose beads (Sigma) for 2 h at 4°C. Immunopellets were washed 4 times with fresh lysis buffer (20mM Tris-HCl (pH 7.4), 100 mM NaCl, 1 mM MgCl2, 0.1% NP40, protease inhibitor cocktail (Roche), and PhosSTOP (Roche)) and eluted in 5x Laemmli buffer. Precipitated proteins were separated by SDS-PAGE and transferred to nitrocellulose membranes. Co-immunoprecipitated proteins were detected by immunoblotting with antibodies recognizing FLAG, 6x-His (ThermoFisher Scientific), Hsp90-835-16F1, GAPDH, p23 (ENZO Life Sciences), Tsc1, FLCN, GR, Myc, V5, GAPDH, Hsp90α, FNIP2, c-Src (Cell Signalling), phospho-tyrosine, v-Src (Millipore), FNIP1, FNIP2 (NCI), FNIP1 (antibodies-online.com), Aha1 (StressMarq Biosciences), HA (Roche). Secondary antibodies raised against mouse, rabbit, and rat (Cell Signaling) and goat (Santa Cruz Biotechnology) were used (See Key resources table).
Backe S.J., Sager R.A., Regan B.R., Sit J., Major L.A., Bratslavsky G., Woodford M.R., Bourboulia D, & Mollapour M. (2022). A specialized Hsp90 co-chaperone network regulates steroid hormone receptor response to ligand. Cell reports, 40(2), 111039.
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