NCI-N87 cells or SGC-7901 cells were seeded in 6-well plates and transiently transfected with plasmid DNA or siRNA. Two days after transfection, the cells were treated with certain concentrations of lapatinib for indicated time points. Both floating and adherent cells were harvested and stained with Annexin V and Propidium iodide (Dojindo, Kumamoto, Japan) and further analyzed with a flow cytometry (FACScan, BD Biosciences, USA) equipped with a Cell Quest software (BD Biosciences, USA). Apoptosis was also determined using the TUNEL apoptotic cell detection kit (Roche, Basel, Switzerland), according to the manufacturer's instructions.
Tunel apoptotic cell detection kit
Manufactured by Roche
Sourced in Switzerland
The TUNEL apoptotic cell detection kit is a laboratory tool used to identify and quantify cells undergoing programmed cell death or apoptosis. It provides a method for the detection and analysis of DNA fragmentation, a hallmark of the apoptotic process.
Automatically generated - may contain errors
Lab products found in correlation
Facscan flow cytometer, by BD (2 mentions)
7 aad, by BD (2 mentions)
Propidium iodide, by Dojindo Laboratories (1 mentions)
Annexin 5, by Dojindo Laboratories (1 mentions)
Cellquest software, by BD (1 mentions)
Facscan, by BD (1 mentions)
Annexin 5 alexa fluor 647, by BD (1 mentions)
Zen le software, by Zeiss (1 mentions)
Lsm 710 confocal microscope system, by Zeiss (1 mentions)
Lsm 700 confocal microscope, by Zeiss (1 mentions)
6 protocols using tunel apoptotic cell detection kit
1
Apoptosis Induction in Cancer Cells
2
Apoptosis Evaluation via Flow Cytometry and TUNEL
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Flow cytometry and Terminal-deoxynucleoitidyl Transferase Mediated Nick End Labeling (TUNEL) were used to analyze cell apoptosis. For flow cytometry, the cells were stained with Alexa Fluor 647 Annexin V and 7-AAD (Becton Dickinson, Franklin Lakes, NJ, USA) according to the manufacturer's instructions. The cells were then tested on a FACScan flow cytometer (Becton Dickinson). Upper (late apoptotic cells) and lower (early apoptotic cells) right quadrants are counted. TUNEL assays were conducted using the TUNEL apoptotic cell detection kit (Roche, Basel, Switzerland), according to the manufacturer's instructions.
3
Apoptosis Analysis via Flow Cytometry and TUNEL
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Flow cytometry and terminal-deoxynucleoitidyl transferase-mediated nick end labeling (TUNEL) were used to analyze cell apoptosis. Flow cytometry was used to analyze cell apoptosis. The cells were treated with norepinephrine for 24 h prior to being harvested. The staining of apoptotic cells was achieved by incubating the cells with7-AAD and Annexin V-Alexa Fluor 647 (BD Biosciences, Franklin Lakes, NJ, USA) in the dark for 15 min at room temperature. The cells were then examined on a FACScan flow cytometer (BD Biosciences). All procedures were performed in triplicate. TUNEL assays were conducted using the TUNEL apoptotic cell detection kit (Roche), according to the manufacturer's instructions.
4
TUNEL Assay for Apoptosis Quantification
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Apoptosis was determined using the TUNEL apoptotic cell detection kit (Roche, Basel, Switzerland), according to the manufacturer's instructions. The percentages of apoptotic cells were counted from at least 1000 cells. The confocal images of cells were sequentially acquired with Zeiss AIM software on a Zeiss LSM 710 confocal microscope system (Carl Zeiss Jena, Oberkochen, Germany) and images were processed with ZEN LE software (Carl Zeiss).
5
Apoptosis Detection by TUNEL Assay
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Apoptosis was determined using the TUNEL apoptotic cell detection kit (Roche, Basel, Switzerland), according to the manufacturer's instructions. The percentages of apoptotic cells were counted from at least 1000 cells.
6
Apoptosis Quantification by TUNEL Assay
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Apoptosis was determined using the TUNEL apoptotic cell detection kit (Roche, Basel, Switzerland), according to the manufacturer's instructions. The percentages of apoptotic cells were counted from at least 1000 cells.The confocal images of cells were sequentially acquired with Zeiss AIM software on a Zeiss LSM 700 confocal microscope system (Carl Zeiss Jena, Oberkochen, Germany).
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