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Quantiscript kit

Manufactured by Qiagen
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The Quantiscript kit is a laboratory product manufactured by Qiagen. It is designed for the reverse transcription of RNA samples into complementary DNA (cDNA) for further analysis. The kit provides the necessary reagents and enzymes to facilitate this conversion process.

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Lab products found in correlation

Rneasy mini kit, by Qiagen (5 mentions) Microamp optical plate, by Thermo Fisher Scientific (5 mentions) Abi prism 7300 sequence, by Thermo Fisher Scientific (3 mentions) Abi prism 7300, by Thermo Fisher Scientific (2 mentions) Cxcl1, by Thermo Fisher Scientific (1 mentions) Cxcl2, by Thermo Fisher Scientific (1 mentions) Mmp 1, by Thermo Fisher Scientific (1 mentions) β actin, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Quantiscript Reverse Transcription Kit (8 citations) Quantiscript Reverse Transcriptase kit (4 citations) Quantiscript (3 citations)

5 protocols using quantiscript kit

1

RT-PCR Analysis of Gene Expression

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Total RNA was isolated by RNeasy Mini Kits (Qiagen, Inc.; Valencia, CA) according to the manufacturer’s protocol. One microgram of RNA was reverse-transcribed using the Qiagen Quantiscript kit according to manufacturers protocol. RT-PCR was performed and analyzed by the dual-labeled fluorogenic probe method by using an ABI Prism 7300 sequence detector (Applied Biosystems; Foster City, CA). Primers and probes for human FLG, actin, STAT6, and ADAM10 were purchased from Applied Biosystems. Amplification reactions were performed in MicroAmp optical plates (Applied Biosystems) in a 25-μL volume as previously described (Howell et al., 2007 (link)). All reactions were normalized to beta actin.
Brauweiler A.M., Goleva E, & Leung D.Y. (2014). Th2 cytokines increase Staphylococcus aureus alpha toxin induced keratinocyte death through the signal transducer and activator of transcription 6 (STAT6). The Journal of investigative dermatology, 134(8), 2114-2121.
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2

Quantitative Real-Time RT-PCR Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total RNA was isolated using RNeasy Mini Kits (Qiagen, Inc.; Valencia, CA) according to the manufacturer’s protocol. One microgram of RNA was reverse transcribed using the Qiagen Quantiscript kit according to manufacturers protocol. RT-PCR was performed and analyzed by the dual-labeled fluorogenic probe method by using an ABI Prism 7300 sequence detector (Applied Biosystems; Foster City, CA). Primers and probes for human Actin, STAT1, Serpin B3, ApoL1 and ADAM10 were purchased from Applied Biosystems. Amplification reactions were performed in MicroAmp optical plates (Applied Biosystems) in a 25-μL volume as previously described (Howell 2007). All reactions were normalized to beta actin.
Brauweiler A.M., Goleva E, & Leung D.Y. (2015). Interferon-γ protects from staphylococcal alpha toxin induced keratinocyte death through apolipoprotein L1. The Journal of investigative dermatology, 136(3), 658-664.
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3

Quantitative Real-Time RT-PCR Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total RNA was isolated using RNeasy Mini Kits (Qiagen, Inc.; Valencia, CA) according to the manufacturer’s protocol. One microgram of RNA was reverse transcribed using the Qiagen Quantiscript kit according to manufacturers protocol. RT-PCR was performed and analyzed by the dual-labeled fluorogenic probe method by using an ABI Prism 7300 sequence detector (Applied Biosystems; Foster City, CA). Primers and probes for human Actin, STAT1, Serpin B3, ApoL1 and ADAM10 were purchased from Applied Biosystems. Amplification reactions were performed in MicroAmp optical plates (Applied Biosystems) in a 25-μL volume as previously described (Howell 2007). All reactions were normalized to beta actin.
Brauweiler A.M., Goleva E, & Leung D.Y. (2015). Interferon-γ protects from staphylococcal alpha toxin induced keratinocyte death through apolipoprotein L1. The Journal of investigative dermatology, 136(3), 658-664.
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4

RT-PCR Analysis of Gene Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total RNA was isolated by RNeasy Mini Kits (Qiagen, Inc.; Valencia, CA) according to the manufacturer’s protocol. One microgram of RNA was reverse-transcribed using the Qiagen Quantiscript kit according to manufacturers protocol. RT-PCR was performed and analyzed by the dual-labeled fluorogenic probe method by using an ABI Prism 7300 sequence detector (Applied Biosystems; Foster City, CA). Primers and probes for human FLG, actin, STAT6, and ADAM10 were purchased from Applied Biosystems. Amplification reactions were performed in MicroAmp optical plates (Applied Biosystems) in a 25-μL volume as previously described (Howell et al., 2007 (link)). All reactions were normalized to beta actin.
Brauweiler A.M., Goleva E, & Leung D.Y. (2014). Th2 cytokines increase Staphylococcus aureus alpha toxin induced keratinocyte death through the signal transducer and activator of transcription 6 (STAT6). The Journal of investigative dermatology, 134(8), 2114-2121.
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5

Quantitative RT-PCR for Cytokine Expression

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Total RNA was isolated by RNeasy Mini Kits (Qiagen, Valencia CA) according to the manufacturer’s protocol. One microgram of RNA was reverse transcribed using the Qiagen Quantiscript kit according to manufacturers protocol. RT-PCR was performed and analyzed by the dual-labeled fluorogenic probe method by using an ABI Prism 7300 sequence detector (Applied Biosystems, Foster City CA). Probes for human IL-1a, IL-1b, IL-8, IL-33, IL-36a, CXCL1, Cxcl2, MMP-1, and beta actin as well as mouse Il-1a, Il-1b, Il-36a, CXCL1, Cxcl2, Ly-6G and beta actin were purchased from Applied Biosystems. Amplification reactions were performed in MicroAmp optical plates (Applied Biosystems) in a 25-μL volume. All reactions were normalized to beta actin.
Brauweiler A.M., Goleva E, & Leung D.Y. (2019). Staphylococcus aureus Lipoteichoic Acid Damages the Skin Barrier through an IL-1 mediated Pathway. The Journal of investigative dermatology, 139(8), 1753-1761.e4.
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