All cell lines were purchased from American Type Culture Collection (ATCC) and cultured according to protocol provide by ATCC at 37°C in 5% CO2. Following primary rabbit polyclonal antibodies were used: anti-α-actinin-1 (A1-A341 [32 (link)]), anti-α-actinin-4 (ALX-210-356, Alexis Biochemicals), anti-laminin (L9393, Sigma-Aldrich), anti-GFP (ab290, Abcam), anti-pMLC (#3671, Cell Signaling), anti-E-cadherin (24E10, #3195, Cell Signaling). Primary mouse monoclonal antibodies were: anti-E-cadherin (HECD-1, ab1416, Abcam), anti-ER-α antibody (sc-8002, Santa Cruz Biotechnology), anti-vinculin (V9131, Sigma-Aldrich). In addition the used primary antibodies were: rat monoclonal anti-E-cadherin antibody (U3254, Sigma-Aldrich), rabbit monoclonal anti-GAPDH (2118S, Cell Signaling), mouse monoclonal anti-β-actin antibody (A1978, Sigma). Secondary antibodies in western blotting were anti-rabbit-HRP and anti-mouse-HRP (Chemicon International) and in immunofluorescence Alexa Fluor® anti-rabbit, anti-mouse or anti-rat (Invitrogen). Filamentous actin was stained with Alexa Fluor® 488, 546 or 647 phalloidin (Invitrogen).
Anti er α antibody sc 8002
Manufactured by Santa Cruz Biotechnology
Sourced in United States
The Anti-ER-α antibody (sc-8002) is a laboratory reagent produced by Santa Cruz Biotechnology. It is designed to detect the estrogen receptor alpha (ER-α) protein in various experimental applications.
Automatically generated - may contain errors
Lab products found in correlation
Anti vinculin v9131, by Merck Group (1 mentions)
Anti gfp ab290, by Abcam (1 mentions)
Anti laminin l9393, by Merck Group (1 mentions)
Anti e cadherin antibody, by Merck Group (1 mentions)
Anti β actin antibody, by Merck Group (1 mentions)
Anti pmlc, by Cell Signaling Technology (1 mentions)
Anti e cadherin 24e10, by Cell Signaling Technology (1 mentions)
Anti gapdh, by Cell Signaling Technology (1 mentions)
2 protocols using anti er α antibody sc 8002
1
Characterization of Cytoskeletal Proteins
2
Immunohistochemical Analysis of ER-α in NPs
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Stored formalin-fixed and paraffin-embedded samples taken from NPs and inferior turbinate tissues were used for immunohistochemical staining. To perform immunohistochemical staining for ER-α, a 4-μm-thick paraffin block was deparaffinized and cleared in xylene, rehydrated with ethanol, and incubated in citrate buffer (0.01 M, pH 6.0), with subsequent heating using an microwave for 15 minutes. The sections were incubated overnight at 4°C with anti-ERα antibody (sc-8002; Santa Cruz Biotechnology, Dallas, TX, USA), followed by incubation in biotin-labeled secondary antibody for 30 minutes and then streptavidin-peroxidase for another 30 minutes. The staining was visualized using 3,3′-diaminobenzidine tetrahydrochloride.
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