The primary antibodies used for western blotting were; rabbit anti-human SOD1 antibodies raised against peptides corresponding to aa 24–39 (2.3 μg/ml), aa 57–72 (1.6 μg/ml), aa 144–153 (5.2 μg/ml) and aa 123–127 GQRWK (4.8 µg/ml, G127X-specific) [46 (link)], rabbit anti-CCS raised against peptides corresponding to aa 252–270 of the human CCS sequence (CCS, 0.9 μg/ml) [46 (link)], rabbit anti-CCS (1:1000 Santa Cruz Biotechnology, Dallas, TX, USA), rabbit anti-glutaredoxin-1 (1:250, Abcam, Cambridge, UK), mouse anti-β-actin (1:200,000; Millipore, Bedford, MA, USA) and rabbit anti-GAPDH (1:1000, Abcam, Cambridge, UK).
Chemidoc touch apparatus
The ChemiDoc Touch apparatus is a digital imaging system designed for capturing and analyzing images of gel-based applications, such as western blots, nucleic acid gels, and protein gels. The core function of this equipment is to provide high-quality image acquisition and analysis capabilities for researchers and scientists working in various fields of molecular biology and biochemistry.
Lab products found in correlation
3 protocols using chemidoc touch apparatus
Western Blot Analysis of Antioxidant Proteins
The primary antibodies used for western blotting were; rabbit anti-human SOD1 antibodies raised against peptides corresponding to aa 24–39 (2.3 μg/ml), aa 57–72 (1.6 μg/ml), aa 144–153 (5.2 μg/ml) and aa 123–127 GQRWK (4.8 µg/ml, G127X-specific) [46 (link)], rabbit anti-CCS raised against peptides corresponding to aa 252–270 of the human CCS sequence (CCS, 0.9 μg/ml) [46 (link)], rabbit anti-CCS (1:1000 Santa Cruz Biotechnology, Dallas, TX, USA), rabbit anti-glutaredoxin-1 (1:250, Abcam, Cambridge, UK), mouse anti-β-actin (1:200,000; Millipore, Bedford, MA, USA) and rabbit anti-GAPDH (1:1000, Abcam, Cambridge, UK).
Quantitative Western Blot Analysis of Muscle Proteins
Quantifying Tissue Protein Levels by Western Blot
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