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Discovery chromomap dab

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The Discovery ChromoMap DAB is a laboratory equipment product. It is used for chromogenic detection in immunohistochemistry and in situ hybridization procedures. The product enables the visualization of target molecules in tissue sections or cell samples.

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Lab products found in correlation

Tissue tek vip 6, by Sakura Finetek (8 mentions) Discovery ultra automated staining instrument, by Roche (4 mentions) Discovery ultra automated, by Roche (2 mentions) Ultramap anti rabbit hrp, by Roche (1 mentions) Discovery ultra, by Roche (1 mentions) Mers cov nucleocapsid protein rabbit antibody, by Sino Biological (1 mentions) Discovery ultra automated ihc ish staining instrument, by Roche (1 mentions) Micromount, by Leica (1 mentions)

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DISCOVERY ChromoMap DAB Kit (28 citations) ChromoMap DAB (23 citations) Discovery ChromoMap DAB detection kit (8 citations) Discovery ChromoMap DAB RUO (5 citations)

9 protocols using discovery chromomap dab

1

Histological Analysis of SARS-CoV-2 Lung Pathology

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Necropsies and tissue sampling were performed according to IBC-approved SOPs. Lungs were perfused with 10% formalin and processed for histologic review. Harvested tissues were fixed for eight days in 10% neutral-buffered formalin, embedded in paraffin, processed using a VIP-6 Tissue Tek (Sakura Finetek, USA) tissue processor, and embedded in Ultraffin paraffin polymer (Cancer Diagnostics, Durham, NC). Samples were sectioned at 5 μm, dried overnight at 42 °C, and resulting slides were stained with hematoxylin and eosin. Specific anti-CoV immunoreactivity was detected using an in-house SARS-CoV-2 nucleocapsid protein (U864YFA140–4/CB2093) rabbit antibody (Genscript) at a 1:1000 dilution. The IHC assay was carried out on a Discovery ULTRA automated staining instrument (Roche Tissue Diagnostics) with a Discovery ChromoMap DAB (Ventana Medical Systems) kit. All tissue slides were evaluated by a board-certified veterinary pathologist. Sections taken at 3 levels from each lung lobe, totally 18 sections, were evaluated for each animal; a representative lesion from each group was selected for Fig. 3.
Furuyama W., Shifflett K., Pinski A.N., Griffin A.J., Feldmann F., Okumura A., Gourdine T., Jankeel A., Lovaglio J., Hanley P.W., Thomas T., Clancy C.S., Messaoudi I., O’Donnell K.L, & Marzi A. (2021). Rapid protection from COVID-19 in nonhuman primates vaccinated intramuscularly but not intranasally with a single dose of a recombinant vaccine. bioRxiv.
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2

Immunohistochemical Profiling of ALK, ROS1, and c-Met

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Alk and Ros1 IHC was performed on 0.6 mm tissue cylinders as previously described [16 (link),17 (link)]. For Alk IHC, the mouse anti-human ALK monoclonal antibody was applied (clone 5A4, Leica Biosystems). Ros1 IHC was conducted using a rabbit anti-human ROS1 monoclonal antibody (clone D4D6, Cell Signaling Technology). For c-Met IHC a monoclonal rabbit anti-human Met antibody was used (clone SP44, Spring Biosciences). All buffers, including pretreatment CC1 standard incubation buffer, secondary antibody (UltraMap anti-Rabbit HRP) and detection system Discovery ChromoMap DAB, were purchased from Roche Ventana (Tucson, AZ). Immunostainings were performed on the automated immunostainer DiscoveryUltra (Roche Ventana).
Velizheva N.P., Rechsteiner M.P., Valtcheva N., Freiberger S.N., Wong C.E., Vrugt B., Zhong Q., Wagner U., Moch H., Hillinger S., Schmitt-Opitz I., Soltermann A., Wild P.J, & Tischler V. (2018). Targeted next-generation-sequencing for reliable detection of targetable rearrangements in lung adenocarcinoma—a single center retrospective study. Pathology, Research and Practice, 214(4), 572-578.
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3

Histological Lung Analysis of MERS-CoV Infection

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Necropsies and tissue sampling were performed according to IBC-approved protocols. Lungs were perfused with 10% formalin and processed for histologic review. Harvested tissues were fixed for a minimum of seven days in 10% neutral-buffered formalin and then embedded in paraffin. Tissues were processed using a VIP-6 Tissue Tek, (Sakura Finetek, USA) tissue processor and embedded in Ultraffin paraffin polymer (Cancer Diagnostics, Durham, NC). Samples were sectioned at 5 μm, and resulting slides were stained with hematoxylin and eosin. Specific anti-CoV immunoreactivity was detected using MERS-CoV nucleocapsid protein rabbit antibody (Sino Biological Inc.) at a 1:4000. The tissues were processed for immunohistochemistry using the Discovery ULTRA automated IHC/ISH staining instrument (Ventana Medical Systems) with a Discovery ChromoMap DAB (Ventana Medical Systems) kit, scanned with the Aperio ScanScope AT2 (Aperio Technologies, Inc.) and the entire section analyzed with the ImageScope Positive Pixel Count algorithm (version 9.1). All tissue slides were evaluated by a board-certified veterinary anatomic pathologist.
van Doremalen N., Letko M., Fischer R.J., Bushmaker T., Yinda C.K., Schulz J., Seifert S.N., Kim N.J., Hemida M.G., Kayali G., Park W.B., Perera R.A., Tamin A., Thornburg N.J., Tong S., Queen K., van Kerkhove M.D., Choi Y.K., Oh M.D., Assiri A.M., Peiris M., Gerber S.I, & Munster V.J. (2021). Surface-aerosol stability and pathogenicity of diverse MERS-CoV strains from 2012 – 2018. bioRxiv.
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4

Immunohistochemical Detection of Ebolavirus

Cited 1 time
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Necropsies and tissue sampling were performed according to IBC-approved SOPs. Harvested tissues were fixed for eight days in 10% neutral-buffered formalin, embedded in paraffin, processed using a VIP-6 Tissue Tek (Sakura Finetek, USA) tissue processor, and embedded in Ultraffin paraffin polymer (Cancer Diagnostics, Durham, NC). Samples were sectioned at 5 μm, dried overnight at 42°C, and resulting slides were stained with heamatoxylin and eosin. Specific anti-VP40 immunoreactivity was detected using a previously described cross-reactive anti-EBOV VP40 antibody (a generous gift by Dr. Yoshihiro Kawaoka, University of Wisconsin–Madison) at a 1:1000 dilution [17 (link),18 (link)]. The immunohistochemical assay was carried out on a Discovery ULTRA automated staining instrument (Roche Tissue Diagnostics) with a Discovery ChromoMap DAB (Ventana Medical Systems) kit. All tissue slides were evaluated by a board-certified veterinary pathologist.
Fletcher P., O’Donnell K.L., Doratt B.M., Malherbe D.C., Clancy C.S., Rhoderick J.F., Feldmann F., Hanley P.W., Ksiazek T.G., Geisbert T.W., Messaoudi I, & Marzi A. (2023). Single-dose VSV-based vaccine protects cynomolgus macaques from disease after Taï Forest virus infection. Emerging Microbes & Infections, 12(2), 2239950.
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5

SARS-CoV-2 Lung Histology and Immunohistochemistry

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Necropsies and tissue sampling were performed according to IBC-approved protocols. Lungs were perfused with 10% formalin and processed for histologic review. Harvested tissues were fixed for eight days in 10% neutral-buffered formalin, embedded in paraffin, processed using a VIP-6 Tissue Tek (Sakura Finetek, USA) tissue processor, and embedded in Ultraffin paraffin polymer (Cancer Diagnostics, Durham, NC). Samples were sectioned at 5 µm, and resulting slides were stained with hematoxylin and eosin. Specific anti-CoV immunoreactivity was detected using an in-house SARS-CoV-2 nucleocapsid protein rabbit antibody (Genscript) at a 1:1000 dilution. The IHC assay was carried out on a Discovery ULTRA automated staining instrument (Roche Tissue Diagnostics) with a Discovery ChromoMap DAB (Ventana Medical Systems) kit. All tissue slides were evaluated by a board-certified veterinary anatomic pathologist blinded to study group allocations. 18 sections, taken from 6 different lung lobes are evaluated for each animal; a representative lesion from each group was selected for the figure.
van Doremalen N., Lambe T., Spencer A., Belij-Rammerstorfer S., Purushotham J.N., Port J.R., Avanzato V.A., Bushmaker T., Flaxman A., Ulaszewska M., Feldmann F., Allen E.R., Sharpe H., Schulz J., Holbrook M., Okumura A., Meade-White K., Pérez-Pérez L., Edwards N.J., Wright D., Bissett C., Gilbride C., Williamson B.N., Rosenke R., Long D., Ishwarbhai A., Kailath R., Rose L., Morris S., Powers C., Lovaglio J., Hanley P.W., Scott D., Saturday G., de Wit E., Gilbert S.C, & Munster V.J. (2020). ChAdOx1 nCoV-19 vaccine prevents SARS-CoV-2 pneumonia in rhesus macaques. Nature, 586(7830), 578-582.
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6

Histological Evaluation of SARS-CoV-2 Lung Infection

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Necropsies and tissue sampling were performed according to IBC-approved SOPs. Lungs were perfused with 10% formalin and processed for histologic review. Harvested tissues were fixed for 8 days in 10% neutral buffered formalin, embedded in paraffin, processed using a VIP-6 Tissue Tek (Sakura Finetek, USA) tissue processor, and embedded in Ultraffin paraffin polymer (Cancer Diagnostics, Durham, NC). Samples were sectioned at 5 μm and dried overnight at 42°C, and the resulting slides were stained with hematoxylin and eosin (H&E). Specific anti-CoV immunoreactivity was detected using an in-house SARS-CoV-2 nucleocapsid protein (U864YFA140-4/CB2093) rabbit antibody (GenScript) at a 1:1,000 dilution. The immunohistochemistry (IHC) assay was carried out on a Discovery Ultra automated staining instrument (Roche Tissue Diagnostics) with a Discovery ChromoMap DAB (Ventana Medical Systems) kit. All tissue slides were evaluated by a board-certified veterinary pathologist. Sections taken at 3 levels from each lung lobe, in total, 18 sections, were evaluated for each animal; a representative lesion from each group was selected for Fig. 2. Lung sections were analyzed for evidence of septal edema and infiltrates representing interstitial pneumonia and were assigned the following scores: 0, normal; 1, minimal; 2, mild; 3, moderate; and 4, severe.
Furuyama W., Shifflett K., Pinski A.N., Griffin A.J., Feldmann F., Okumura A., Gourdine T., Jankeel A., Lovaglio J., Hanley P.W., Thomas T., Clancy C.S., Messaoudi I., O’Donnell K.L, & Marzi A. (2022). Rapid Protection from COVID-19 in Nonhuman Primates Vaccinated Intramuscularly but Not Intranasally with a Single Dose of a Vesicular Stomatitis Virus-Based Vaccine. mBio, 13(1), e03379-21.
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7

Histological Analysis of SARS-CoV-2 Lung Samples

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Necropsies and tissue sampling were performed according to IBC-approved protocols. Lungs were perfused with 10% neutral-buffered formalin and fixed for 8 days. Hereafter, tissue was embedded in paraffin, processed using a VIP 6 Tissue-Tek (Sakura Finetek) tissue processor, and embedded in Ultraffin paraffin polymer (Cancer Diagnostics). Samples were sectioned at 5 μm, deparaffinized in xylene, passed through 100% ethanol, and rehydrated in tap water. Samples were stained with Harris hematoxylin (Cancer Diagnostics, no. SH3777), decolorized with 0.125% HCl/70% ethanol, blued in Pureview PH Blue (Cancer Diagnostics, no. 167020), counterstained with eosin 615 (Cancer Diagnostics, no. 16601), dehydrated, and mounted in Micromount (Leica, no. 3801731), coverslipping medium at room temperature. An in-house–generated SARS-CoV-2 nucleocapsid protein rabbit antibody (GenScript) at a 1:1000 dilution was used to detect specific anti–SARS-CoV-2 immunoreactivity, carried out on a Discovery ULTRA automated staining instrument (Roche Tissue Diagnostics) with a Discovery ChromoMap DAB (Ventana Medical Systems) kit. The tissue slides were examined by a board-certified veterinary anatomic pathologist blinded to study group allocations. Eighteen sections, taken from six different lung lobes, were evaluated for each animal; a representative lesion from each group was selected for figures.
van Doremalen N., Purushotham J.N., Schulz J.E., Holbrook M.G., Bushmaker T., Carmody A., Port J.R., Yinda C.K., Okumura A., Saturday G., Amanat F., Krammer F., Hanley P.W., Smith B.J., Lovaglio J., Anzick S.L., Barbian K., Martens C., Gilbert S.C., Lambe T, & Munster V.J. (2021). Intranasal ChAdOx1 nCoV-19/AZD1222 vaccination reduces viral shedding after SARS-CoV-2 D614G challenge in preclinical models. Science Translational Medicine, 13(607), eabh0755.
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8

SARS-CoV-2 Histopathological Analysis

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Necropsies and tissue sampling were performed according to IBC-approved protocols. Lungs were perfused with 10% formalin and processed for histologic review. Harvested tissues were fixed for eight days in 10% neutral-buffered formalin, embedded in paraffin, processed using a VIP-6 Tissue Tek (Sakura Finetek, USA) tissue processor, and embedded in Ultraffin paraffin polymer (Cancer Diagnostics, Durham, NC). Samples were sectioned at 5 μm, and resulting slides were stained with hematoxylin and eosin. Specific anti-CoV immunoreactivity was detected using an in-house SARS-CoV-2 nucleocapsid protein rabbit antibody (Genscript) at a 1:1000 dilution. The IHC assay was carried out on a Discovery ULTRA automated staining instrument (Roche Tissue Diagnostics) with a Discovery ChromoMap DAB (Ventana Medical Systems) kit. All tissue slides were evaluated by a board-certified veterinary anatomic pathologist blinded to study group allocations.
van Doremalen N., Lambe T., Spencer A., Belij-Rammerstorfer S., Purushotham J.N., Port J.R., Avanzato V., Bushmaker T., Flaxman A., Ulaszewska M., Feldmann F., Allen E.R., Sharpe H., Schulz J., Holbrook M., Okumura A., Meade-White K., Pérez-Pérez L., Bissett C., Gilbride C., Williamson B.N., Rosenke R., Long D., Ishwarbhai A., Kailath R., Rose L., Morris S., Powers C., Lovaglio J., Hanley P.W., Scott D., Saturday G., de Wit E., Gilbert S.C, & Munster V.J. (2020). ChAdOx1 nCoV-19 vaccination prevents SARS-CoV-2 pneumonia in rhesus macaques. bioRxiv.
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9

SARS-CoV-2 Lung Histology and Immunohistochemistry

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Necropsies and tissue sampling were performed according to IBC-approved protocols. Lungs were perfused with 10% formalin and processed for histologic review. Harvested tissues were fixed for eight days in 10% neutral-buffered formalin, embedded in paraffin, processed using a VIP-6 Tissue Tek (Sakura Finetek, USA) tissue processor, and embedded in Ultraffin paraffin polymer (Cancer Diagnostics, Durham, NC). Samples were sectioned at 5 µm, and resulting slides were stained with hematoxylin and eosin. Specific anti-CoV immunoreactivity was detected using an in-house SARS-CoV-2 nucleocapsid protein rabbit antibody (Genscript) at a 1:1000 dilution. The IHC assay was carried out on a Discovery ULTRA automated staining instrument (Roche Tissue Diagnostics) with a Discovery ChromoMap DAB (Ventana Medical Systems) kit. All tissue slides were evaluated by a board-certified veterinary anatomic pathologist blinded to study group allocations. 18 sections, taken from 6 different lung lobes are evaluated for each animal; a representative lesion from each group was selected for the figure.
van Doremalen N., Lambe T., Spencer A., Belij-Rammerstorfer S., Purushotham J.N., Port J.R., Avanzato V.A., Bushmaker T., Flaxman A., Ulaszewska M., Feldmann F., Allen E.R., Sharpe H., Schulz J., Holbrook M., Okumura A., Meade-White K., Pérez-Pérez L., Edwards N.J., Wright D., Bissett C., Gilbride C., Williamson B.N., Rosenke R., Long D., Ishwarbhai A., Kailath R., Rose L., Morris S., Powers C., Lovaglio J., Hanley P.W., Scott D., Saturday G., de Wit E., Gilbert S.C, & Munster V.J. (2020). ChAdOx1 nCoV-19 vaccine prevents SARS-CoV-2 pneumonia in rhesus macaques. Nature, 586(7830), 578-582.
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