Anti digoxigenin rhodamine red

Manufactured by Roche

Sourced in United States

Anti-digoxigenin-rhodamine (red) is a fluorescent probe used in various laboratory techniques. It binds to digoxigenin, a hapten commonly used for labeling and detection in molecular biology applications. The rhodamine dye attached to the anti-digoxigenin provides a red fluorescent signal that can be detected and visualized.

Automatically generated - may contain errors

Lab products found in correlation

Vectashield solution, by Vector Laboratories (2 mentions) Avidin fluorescein green, by Roche (2 mentions) Digoxigenin 11 dutp, by Roche (1 mentions) Vectashield antifade solution, by Vector Laboratories (1 mentions) Imager m1 microscope, by Zeiss (1 mentions) Axioskop 2 plus, by Zeiss (1 mentions)

Spelling variants of this product

Anti-digoxigenin-rhodamine (41 citations)

4 protocols using anti digoxigenin rhodamine red

Dual-Color Fluorescence In Situ Hybridization for Metaphase Chromosomes

Check if the same lab product or an alternative is used in the 5 most similar protocols

Dual-color FISH (fluorescence in situ hybridization) and the detection of metaphase chromosome specimens were performed according to a previous [36 (link)]. The second round of LA-PCR products labeled with DIG (Digoxigenin-11-dUTP, Roche) and specific Biotin (Biotin-16-dUTP) labeled bacterial artificial chromosome (BAC) clones (52D06) were used as probes, which were detected by Anti-Digoxigenin-Rhodamine (red) and FITC-Anti-Biotin (green) (Roche Diagnostics, USA), respectively. Cot-1 DNA was used to pre-hybridize for blocking the repetitive sequences. Chromosomes were counterstained by 4′, 6-diamidino-2-phenylindole (DAPI) in VECTASHIELD anti-fade solution (Vector Laboratories, Burlingame, CA). The hybridization signals were observed using a fluorescence microscope with a change-coupled device (CCD) camera (Zeiss Axiokop2 plus). The images were adjusted using Adobe Photoshop CS3 software.

Cao X., Liu Y., Liu Z., Liu F., Wu Y., Zhou Z., Cai X., Wang X., Zhang Z., Wang Y., Luo Z., Peng R, & Wang K. (2017). Microdissection of the Ah01 chromosome in upland cotton and microcloning of resistance gene anologs from the single chromosome. Hereditas, 154, 13.

+ Open protocol

+ Expand

Chromosome Preparation and FISH Procedure

Check if the same lab product or an alternative is used in the 5 most similar protocols

Chromosome Preparation and the FISH procedure were conducted according to the previous protocols [45 (link), 46 ]. The probes were detected with anti-digoxigenin-rhodamine (red) (Roche, USA). Images were captured using a CCD camera attached to a Zeiss Imager M1 microscope. Images were processed using Photoshop CS3.

Liu Y., Peng R., Liu F., Wang X., Cui X., Zhou Z., Wang C., Cai X., Wang Y., Lin Z, & Wang K. (2016). A Gossypium BAC clone contains key repeat components distinguishing sub-genome of allotetraploidy cottons. Molecular Cytogenetics, 9, 27.

+ Open protocol

+ Expand

Mitotic Chromosome FISH Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mitotic chromosome preparation and FISH procedures were conducted using a modified protocol (Wang et al. 2001b ). Biotin-labeled and digoxigenin-labeled probes were detected by avidin-fluorescein (green) and anti-digoxigenin-rhodamine (red) (Roche Diagnostics, USA), respectively. Chromosomes were counterstained by

4’,6-diamidino-2-phenylindole

(DAPI) in antifade VECTASHIELD solutions (Vector Laboratories, Burlingame, CA). The concentration of block DNA (genomic DNA) was 200 times that of the chromosome-specific BAC DNA. The hybridization signals were observed using a fluorescence microscope (Leica MRA2) with a

charge-coupled device

(CCD) camera. Final image adjustments were performed using Adobe Photoshop CS3 software.

Cui X., Liu F., Liu Y., Zhou Z., Wang C., Yanyan Z.h.a.o., Meng F., Wang X., Cai X., Wang Y., Peng R, & Wang K. (2016). Screening and chromosome localization of two cotton BAC clones. Comparative Cytogenetics, 10(1), 1-15.

+ Open protocol

+ Expand

Mitotic Chromosome FISH Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mitotic chromosomes preparation and FISH procedure were conducted using a modified protocol [61 ]. Biotin-labeled and digoxigenin-labeled probes were detected by avidin-fluorescein (green) and anti-digoxigenin-rhodamine (red) (Roche Diagnostics, USA), respectively. Chromosomes were counterstained by 4′,6-diamidino-2-phenylindole (DAPI) in the antifade VECTASHIELD solutions (Vector Laboratories, Burlingame, CA). For the probe-cocktail mixture, gDNA was used as block DNA. The dose of block DNA was 200 times of the chromosome-specific BAC DNA. The hybridization signals were observed using a fluorescence microscope (Leica MRA2) with a charge-coupled device (CCD) camera (Zeiss Axioskop2 plus). Final image adjustments were performed by using Adobe Photoshop CS3 software.

Cui X., Liu F., Liu Y., Zhou Z., Zhao Y., Wang C., Wang X., Cai X., Wang Y., Meng F., Peng R, & Wang K. (2015). Construction of cytogenetic map of Gossypium herbaceum chromosome 1 and its integration with genetic maps. Molecular Cytogenetics, 8, 2.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!