Ct dna

First, we configured 10 mM Tris-HCl (Zhiyuan, Tianjin, China), pH = 7.4 as the buffer solution, dissolved 1 mg/mL CT-DNA (Solarbio, Beijing, China) in 10 mM Tris-HCl, pH = 7.4 buffer solution, and detected the absorbance value of the dissolved CT-DNA solution at a wavelength of 260 nm (ε = 6600 L/M cm). According to the Beer–Lambert–Bouguer law formula:
A = εbc, where ε is the extinction coefficient, b is the optical path length, and c is the solution concentration [42 (link)].
We used 10 mM Tris-HCl, pH = 7.4 as buffer, 90 μM dutomycin, and 120 μM SW91. We added different concentrations of CT-DNA (0–34 μM), diluted to 1 mL with a 1 mL volumetric flask, and detected the UV–Vis spectrum on a Shimadzu UV–Vis spectrophotometer through a quartz cuvette with a 1.0 nm gap width. We scanned the full wavelength using a UV–Vis spectrophotometer to obtain the absorbance curve obtained by gradually increasing the CT-DNA concentration at the same drug concentration.

¹H-NMR spectra were determined with an Agilent Technologies 800/54 premium (Rogowska, Wroclaw, Poland). Elemental analyses were run on an Elementar Vario EL III Elemental Analyzer (Langenselbold, Frankfurt, Germany). IR spectra were recorded on a Nicolet IS10 (Carlsbad, CA, USA). Intensity data of single crystals were collected using an Agilent SuperNova diffractometer (Santa Clara, CA, USA). UV-Vis absorption spectra were performed using a Beckman coulter DU800 (Brea, CA, USA). Fluorescence spectra were recorded on a PerkinElmer LS 55 (Waltham, MA, USA) luminescence spectrometer with a red-sensitive photomultiplier type R928. Circular dichroism (CD) spectra carried out on a Chirascan CD spectropolarimeter (Applied Photophysics, Leatherhead, Surrey, U.K.). All the cells were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA) and Tris-HCl buffer, BSA and CT-DNA were obtained from Beijing Solarbio Science & Technology Co., Ltd., Asbio and Solarbio (Beijing, China), respectively. BSA were dissolved in Tris-HCl buffer and stored at 4 °C. The complexes were dissolved in DMSO to prepare stock solution and the solubility of the complexes was 20 mM/mL in DMSO, while the solubility of 5 was 15 mM/mL. All reagents used in the experiments were of analytical grade or purified by standard methods.