Mmp 9 rabbit mab

Manufactured by Abcam

Sourced in United States

MMP-9 rabbit mAb is a primary antibody that specifically binds to the matrix metalloproteinase-9 (MMP-9) protein. MMP-9 is an enzyme involved in the breakdown of extracellular matrix components. This antibody can be used for the detection and quantification of MMP-9 in various experimental applications.

Automatically generated - may contain errors

Lab products found in correlation

P44 42 mapk erk1 2 rabbit mab, by Cell Signaling Technology (2 mentions) Chloroquine, by Merck Group (1 mentions) Primescript rt master mix perfect real time kit, by Takara Bio (1 mentions) Leibovitz s l 15 medium, by Merck Group (1 mentions) Recombinant human sdf 1α, by Thermo Fisher Scientific (1 mentions) Antibodies against cxcr4, by Abcam (1 mentions) Mg132, by Merck Group (1 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions) Sybr premix ex taq 2, by Takara Bio (1 mentions) Total rna extraction kit, by Fastagen (1 mentions) Revertaidtm first strand cdna synthesis kit, by Thermo Fisher Scientific (1 mentions) Goat anti rabbit igg, by Santa Cruz Biotechnology (1 mentions) Protease inhibitor cocktail, by Roche (1 mentions)

2 protocols using mmp 9 rabbit mab

Investigating SDF-1α/CXCR4 Signaling Pathway

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TPD7 was from the Research and Engineering Center for Natural Medicine, Xi’an Jiaotong University. LEIBOVITZ’S L15 medium, MG132 and chloroquine were purchased from Sigma-Aldrich (St. Louis, MO, USA). Foetal bovine serum (FBS) was obtained from Lanzhou national hyclone Bio-Engineering Co., Ltd (Lanzhou, China). Recombinant human SDF-1α was purchased from PeproTech (Rocky Hill, NJ, USA). Antibodies against CXCR4 were obtained from Abcam (Burlingame, CA, USA). Matrix metalloproteinase (MMP)-2 rabbit mAb and MMP-9 rabbit mAb were obtained from Epitomics (Burlingame, CA, USA). p44/42 MAPK (ERK1/2) rabbit mAb and p-p44/42 MAPK (p-ERK1/2) rabbit mAb were purchased from Cell Signaling (Danvers, MA, USA). Horseradish Peroxidase (HRP)-conjugated Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) monoclonal antibody was from Proteintech Group (Chicago, IL, USA). Total RNA extraction kit was from Fastagen (Fastagen, Shanghai, China). PrimeScript RT Master Mix Perfect Real Time Kit (DRR036A) and SYBR Premix Ex Taq II were from TaKaRa (Dalian, China). Lipofectamine 2000 was from Invitrogen (Carlsbad, CA, USA). Other reagents used were analytical grades.

Zhan Y., Zhang H., Li J., Zhang Y., Zhang J, & He L. (2015). A novel biphenyl urea derivate inhibits the invasion of breast cancer through the modulation of CXCR4. Journal of Cellular and Molecular Medicine, 19(7), 1614-1623.

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Analyzing Intracellular Signaling Pathways

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RNase and PI were obtained from Sigma (USA). Akt rabbit mAb, Raf-1 rabbit mAb, JNK-1 rabbit mAb, MMP-2 rabbit mAb and MMP-9 rabbit mAb were obtained from Epitomics (USA). P-Akt, P- m-TOR, MEK 1/2 rabbit mAb and p44/42 MAPK (Erk1/2) rabbit mAb were purchased from Cell Signaling (USA). PKCβ rabbit PolyAb, MTOR rabbit PolyAb, CXCR4 mouse mAb, NF-κb rabbit PolyAb, PLG rabbit PolyAb, Cyclin B1 rabbit polyAb, cyclin D1 mouse mAb, cyclin E rabbit polyAb and cdc2 rabbit polyAb, HRP-conjugated GAPDH mAb were from proteintech group (USA). Mutated P53 rabbit mAb was from Abcam (England). Goat anti-rabbit IgG were purchased from Santa Cruz Biotechnology (CA, USA). Protease inhibitor cocktail was from Roche (Roche Tech., Switzerland). Rabbit anti-mouse IgG, goat anti-rabbit IgG, BCA protein assay reagent kit and enhanced chemiluminescent (ECL) plus reagent kit were obtained from Pierce (Pierce Biotech, USA). Total RNA extracted kit was from Fastagen (Fastagen, China). Revert AIDTM. first strand cDNA synthesis kit was from Fermentas (Hanover, Lithuania). RNAi was from Fermentas (Hanover, Lithuania). Other reagents used were analytical grade.

Zhang Y., Zhan Y., Zhang D., Dai B., Ma W., Qi J., Liu R, & He L. (2014). Eupolyphaga sinensis Walker displays inhibition on hepatocellular carcinoma through regulating cell growth and metastasis signaling. Scientific Reports, 4, 5518.

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