Monoclonal rabbit anti ldh a antibody

The following antibodies were used: polyclonal rabbit anti-NOX2 (ab129068, Abcam, Cambridge, UK), monoclonal mouse anti-NOX2 (NBP1-41012, Novus), monoclonal rabbit anti-HK2 antibody (#2024), monoclonal rabbit anti-LDH-A antibody (#3582, Cell Signaling Technology), monoclonal rabbit anti-LDH-A antibody (#3582, Cell Signaling Technology), polyclonal rabbit anti-COL5A1 antibody (#37304, Cell Signaling Technology), monoclonal mouse anti-GFAP (#3670, Cell Signaling Technology), monoclonal rabbit anti-GFAP (#12389, Cell Signaling Technology), and monoclonal mouse anti-β-actin (A5316, Sigma-Aldrich). Fluoroshield™ with DAPI (F6057, Sigma-Aldrich) was used for nuclear staining and mounting. Sections were mounted onto gelatin-coated slides with Canada Balsam (Wako, Tokyo, Japan) following dehydration.

The immunoblot analysis was conducted with the following primary antibodies: monoclonal rabbit anti-HK2 antibody (#2024, Cell Signaling Technology, Danvers, MA, USA), monoclonal rabbit anti-LDH-A antibody (#3582, Cell Signaling Technology, Danvers, MA, USA), monoclonal rabbit anti-PFKP antibody (#8164, Cell Signaling Technology, Danvers, MA, USA), monoclonal rabbit anti-PKM2 antibody (#4053, Cell Signaling Technology, Danvers, MA, USA), and monoclonal mouse anti-β-actin (A5316, Sigma-Aldrich, St. Louis, MO, USA). For nuclear staining and mounting in immunofluorescence analysis, tissue slides were processed with Fluoroshield™ with DAPI (F6057, Sigma-Aldrich, St. Louis, MO, USA). For mounting in the immunohistochemistry analysis, tissues were mounted onto gelatin-coated slides and were processed with Canada Balsam (Wako, Tokyo, Japan) following the dehydration of sections.