Immobilon-P PVDF membranes (Millipore) were probed with the primary antibodies at 4°C overnight and then washed and incubated with HRP-conjugated goat anti-mouse IgG (H+L) or anti-rabbit IgG (H+L) secondary antibody (Promega). The following primary antibodies were used: anti-STAT3 (clone D3Z2G, Cell Signaling Technology), anti-phospho-STAT3 (Tyr705) (clone D3A7, Cell Signaling Technology), anti-STAT5 (Cell Signaling Technology), anti-phospho-STAT5 (Tyr694) (clone D47E7, Cell Signaling Technology), anti-β-actin (clone C4, Santa Cruz Biotechnology, sc-47778), HRP-conjugated anti-mouse IgG (H+L) (Promega, W4021), and HRP-conjugated anti-rabbit IgG (H+L) (Promega, W4011). All images were acquired with ChemiDoc MP system (Bio-Rad) and Image Lab software (Bio-Rad). Protein levels for each blot were quantified with ImageJ software.
Anti phospho stat5 tyr694 clone d47e7
Manufactured by Cell Signaling Technology
Anti-phospho-STAT5 (Tyr694) (clone D47E7) is a rabbit monoclonal antibody that specifically recognizes the phosphorylated form of STAT5 at tyrosine 694. This antibody can be used for various applications, including Western blotting, immunoprecipitation, and immunohistochemistry.
Automatically generated - may contain errors
Lab products found in correlation
Anti phospho stat3 tyr705 clone d3a7, by Cell Signaling Technology (2 mentions)
Anti β actin clone c4, by Santa Cruz Biotechnology (2 mentions)
Hrp conjugated anti mouse igg h l, by Promega (2 mentions)
Hrp conjugated anti rabbit igg h l, by Promega (2 mentions)
Anti stat5, by Cell Signaling Technology (2 mentions)
Hrp conjugated goat anti mouse igg h l, by Promega (2 mentions)
Anti rabbit igg h l secondary antibody, by Promega (2 mentions)
Anti stat3 clone d3z2g, by Cell Signaling Technology (2 mentions)
Immobilon p pvdf membrane, by Merck Group (2 mentions)
Image lab software, by Bio-Rad (2 mentions)
Chemidoc mp system, by Bio-Rad (2 mentions)
2 protocols using anti phospho stat5 tyr694 clone d47e7
1
Western Blot Analysis of STAT3 and STAT5
2
Western Blot Analysis of STAT3 and STAT5
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Immobilon-P PVDF membranes (Millipore) were probed with the primary antibodies at 4°C overnight and then washed and incubated with HRP-conjugated goat anti-mouse IgG (H+L) or anti-rabbit IgG (H+L) secondary antibody (Promega). The following primary antibodies were used: anti-STAT3 (clone D3Z2G, Cell Signaling Technology), anti-phospho-STAT3 (Tyr705) (clone D3A7, Cell Signaling Technology), anti-STAT5 (Cell Signaling Technology), anti-phospho-STAT5 (Tyr694) (clone D47E7, Cell Signaling Technology), anti-β-actin (clone C4, Santa Cruz Biotechnology, sc-47778), HRP-conjugated anti-mouse IgG (H+L) (Promega, W4021), and HRP-conjugated anti-rabbit IgG (H+L) (Promega, W4011). All images were acquired with ChemiDoc MP system (Bio-Rad) and Image Lab software (Bio-Rad). Protein levels for each blot were quantified with ImageJ software.
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