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Phospho p44 42 mapk erk1 2 t202 y204 clone d13.14.4e

Manufactured by Cell Signaling Technology
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The Phospho p44/42 MAPK (Erk1/2; T202/Y204) clone D13.14.4E is a rabbit monoclonal antibody that detects endogenous levels of p44/42 MAPK (Erk1/2) when phosphorylated at threonine 202 and tyrosine 204. This antibody is designed for use in western blotting, immunohistochemistry, and other immunoassay applications.

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Lab products found in correlation

Total erk1 2, by Cell Signaling Technology (2 mentions) Total mek1 2, by Cell Signaling Technology (2 mentions) Pan trk clone a7h6r, by Cell Signaling Technology (2 mentions) Phospho trka y674 675 clone c50f3, by Cell Signaling Technology (2 mentions) Phospho plc y783, by Cell Signaling Technology (2 mentions) Phospho mek1 2 s217 221 clone 41g9, by Cell Signaling Technology (2 mentions) Braf clone d9t6s, by Cell Signaling Technology (2 mentions) Brafv600e, by Abcam (2 mentions) Phospho akt s473 clone d9e, by Cell Signaling Technology (2 mentions) Pan ras, by Santa Cruz Biotechnology (2 mentions) β actin clone 13e5, by Cell Signaling Technology (2 mentions)

2 protocols using phospho p44 42 mapk erk1 2 t202 y204 clone d13.14.4e

1

Comprehensive Western Blot Analysis

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Western blots: total protein lysates following the indicated treatment were extracted and separated using SDS-PAGE gels according to standard methods. Membranes were probed using the following antibodies: pan Trk clone A7H6R (92991S Cell Signaling Technology), phospho TrkA (Y674/675) clone C50F3 (4621S Cell Signaling Technology), phospho PLCƔ (Y783; 2821L Cell Signaling Technology), phospho MEK1/2 (S217/221) clone 41G9 (9154S Cell Signaling Technology), total MEK1/2 (9122L Cell Signaling Technology), BRAF clone D9T6S (14814S Cell Signaling Technology), BRAF V600E (ab228461 Abcam), phospho p44/42 MAPK (Erk1/2; T202/Y204) clone D13.14.4E (4370S Cell Signaling Technology), total ERK1/2 (9102S Cell Signaling Technology), phospho AKT (S473) clone D9E (4060L Cell Signaling Technology), total AKT (9272S Cell Signaling Technology), pan RAS (BK008, part AESA02 Cytoskeleton), KRAS (F234 Santa Cruz) and β-actin clone 13E5 (4970S Cell Signaling Technology).
Cocco E., Schram A.M., Kulick A., Misale S., Won H.H., Yaeger R., Razavi P., Ptashkin R., Hechtman J.F., Toska E., Cownie J., Somwar R., Shifman S., Mattar M., Selçuklu S.D., Samoila A., Guzman S., Tuch B.B., Ebata K., de Stanchina E., Nagy R.J., Lanman R.B., Houck-Loomis B., Patel J.A., Berger M.F., Ladanyi M., Hyman D.M., Drilon A, & Scaltriti M. (2019). Resistance to TRK inhibition mediated by convergent MAP kinase pathway activation. Nature medicine, 25(9), 1422-1427.
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2

Comprehensive Western Blot Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Western blots: total protein lysates following the indicated treatment were extracted and separated using SDS-PAGE gels according to standard methods. Membranes were probed using the following antibodies: pan Trk clone A7H6R (92991S Cell Signaling Technology), phospho TrkA (Y674/675) clone C50F3 (4621S Cell Signaling Technology), phospho PLCƔ (Y783; 2821L Cell Signaling Technology), phospho MEK1/2 (S217/221) clone 41G9 (9154S Cell Signaling Technology), total MEK1/2 (9122L Cell Signaling Technology), BRAF clone D9T6S (14814S Cell Signaling Technology), BRAF V600E (ab228461 Abcam), phospho p44/42 MAPK (Erk1/2; T202/Y204) clone D13.14.4E (4370S Cell Signaling Technology), total ERK1/2 (9102S Cell Signaling Technology), phospho AKT (S473) clone D9E (4060L Cell Signaling Technology), total AKT (9272S Cell Signaling Technology), pan RAS (BK008, part AESA02 Cytoskeleton), KRAS (F234 Santa Cruz) and β-actin clone 13E5 (4970S Cell Signaling Technology).
Cocco E., Schram A.M., Kulick A., Misale S., Won H.H., Yaeger R., Razavi P., Ptashkin R., Hechtman J.F., Toska E., Cownie J., Somwar R., Shifman S., Mattar M., Selçuklu S.D., Samoila A., Guzman S., Tuch B.B., Ebata K., de Stanchina E., Nagy R.J., Lanman R.B., Houck-Loomis B., Patel J.A., Berger M.F., Ladanyi M., Hyman D.M., Drilon A, & Scaltriti M. (2019). Resistance to TRK inhibition mediated by convergent MAP kinase pathway activation. Nature medicine, 25(9), 1422-1427.
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