Opsite

Manufactured by Smith & Nephew

Sourced in United Kingdom, United States

Opsite is a transparent, semi-permeable adhesive film dressing designed for wound care. It provides a protective barrier against external contaminants while allowing the passage of moisture vapor and oxygen.

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Lab products found in correlation

Silicone splints, by Grace Bio-Labs (1 mentions) 3 ml syringe, by Terumo (1 mentions) Balb c nude mice, by Orient Bio (1 mentions) Strattice, by Abbvie (1 mentions) Dermabond skin adhesive, by Johnson & Johnson (1 mentions) Surgimend, by Integra LifeSciences (1 mentions) Kl 1500 lcd, by Schott (1 mentions) Durogesic, by Johnson & Johnson (1 mentions) Steri strip, by 3M (1 mentions) Purified bovine thrombin, by Merck Group (1 mentions) Anti cd8 ab, by BioXCell (1 mentions) Metacam, by Boehringer Ingelheim (1 mentions) Xylazine, by Bayer (1 mentions) Zoletil, by Virbac (1 mentions) 6 0 prolene, by Johnson & Johnson (1 mentions)

22 protocols using opsite

Wound Healing with Keratinocyte-Hyaluronic Composites

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Under aseptic conditions, reproducible standardized 2×2 cm rectangular full-thickness skin wounds were created on the back of anaesthetized nude mice. In group A, 12 freshly created wounds were covered with subconfluent monolayers of cultured human keratinocytes on esterified HA membranes as upside-down grafts with the subconfluent keratinocytes directed towards the wound bed and the HYAFF on top (KHAMC=Keratinocyte-Hyaluronic-Acid-Membrane-Composites) or with the HA membrane underneath (conventional technique=upside-up, as propagated by the manufacturer) (group B, n=12) or with the HA membrane alone (group C, n=12) (Figure 3).
The wounds were dressed with a semipermeable adhesive film (Op-Site, Smith&Nephew, Largo, Fl, USA) and tie-over dressing, consisting of a Vaseline gauze (Adaptic, Johnson & Johnson, New Brunswick, NJ, USA) and a dry cotton gauze, fixed with sutures to the wound margins. Daily inspections were performed to ensure the integrity of the dressings. Biopsies were taken on days 7, 14, 21, and 35 after grafting. Wounds were totally excised, including a small border zone of untreated skin and underlying muscle, and processed for HE staining, as well as immunohistochemical and electron microscopy staining procedures.

Horch R.E., Wagner G., Bannasch H., Kengelbach-Weigand A., Arkudas A, & Schmitz M. (2019). Keratinocyte Monolayers on Hyaluronic Acid Membranes as “Upside-Down” Grafts Reconstitute Full-Thickness Wounds. Medical Science Monitor : International Medical Journal of Experimental and Clinical Research, 25, 6702-6710.

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Splinted Dorsal Wound Healing in Mice

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Dorsal wound healing experiments conducted for histological analysis and cell isolations using Foxd1GC/Ai14 mice were performed as above, but were also splinted and wrapped as described previously.²³ A splinted wound model was used to impair rapid wound contraction, necessitating closure through granulation tissue formation and remodeling. The dorsal skin was shaved, and the remaining hair was removed with depilating cream. The 6 mm wounds were created with a biopsy punch and wounds were splinted open with silicone splints (Grace Bio labs; Bend, ORE, USA), which were held in place with a cyanoacrylate adhesive and 6–0 nylon sutures. Wounds were then covered with an adhesive contact layer (Mepitel® One; Mӧlnlycke; Gothenburg, Sweden) and a semiocclusive dressing (OPSITE®; Smith & Nephew; Mississauga, ON, Canada), prior to being wrapped in a self‐adherent bandage and cloth tape. Following surgery, pain was controlled using 0.05 mg/kg buprenorphine delivered intraperitoneally prior to surgery and 6–8 hours following surgery. Dressings were changed every 3–4 days. For the 21‐day time point, bandages and splints were removed after 14 days, at which time the wounds had reepithelialized.

Walker J.T., Flynn L.E, & Hamilton D.W. (2021). Lineage tracing of Foxd1‐expressing embryonic progenitors to assess the role of divergent embryonic lineages on adult dermal fibroblast function. FASEB BioAdvances, 3(7), 541-557.

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Wound Fluid Collection Protocol for CVLU

Cited 2 times

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Wound fluid is collected from unhealed ulcers at each study time point using a standard wound fluid collection protocol [25 (link), 29 (link)]. The fluid is collected by the PI experienced in the protocol or CRC nurses trained by the PI. Briefly, after CVLUs are washed with sterile water, a transparent occlusive film (Opsite, Smith & Nephew, UK) is applied over the wound and the leg is placed in a dependent position for approximately 1–1½ hours. While slowly removing the occlusive film and rinsing the wound with 1 ml of sterile saline, the fluid is collected using a 26G × 0.5″ angiocatheter attached to a 3-ml syringe (Terumo Medical, Somerset, NJ, USA). The fluid is transferred into plain collection tubes and analyzed immediately to determine PMN activation or frozen and stored at − 80^° C until further analysis.

McDaniel J.C., Rausch J, & Tan A. (2020). Impact of omega-3 fatty acid oral therapy on healing of chronic venous leg ulcers in older adults: Study protocol for a randomized controlled single-center trial. Trials, 21, 93.

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Ferret Radiotelemetric Device Implantation

Cited 1 time

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Eight ferrets were fasted overnight but allowed free access to water. The surgical procedures for implanting radiotelemetric devices (C50-PXT; Data Sciences International, United States) have been described in our previous studies (Tu et al., 2017 (link)). Briefly, in the anesthetized animals, following a midline abdominal incision, a 19G needle was used to pierce the aorta, and then the catheter of a C50-PXT transmitter (Data Sciences, Inc, United States) was inserted up to a length of approximately 2 cm. A 2 × 2 mm piece of sterile gauze was placed over the catheter’s entry point, and fixed with a drop of tissue glue. The body of the transmitter was then sutured to the left side of the ferret’s abdominal wall muscle with the biopotential wires and catheter facing caudally. The gastric antrum was exposed and the biopotential wires were inserted into the muscle and secured in place by suturing the serosa. The abdominal cavity was sutured in closed layers and covered with a permeable spray dressing (Opsite^®, Smith and Nephew, United Kingdom). After the surgery, animals were allowed to recover for 7 days before drug treatment.

Lu Z., Zhou Y., Tu L., Chan S.W., Ngan M.P., Cui D., Liu Y.H., Huang I.B., Kung J.S., Hui C.M, & Rudd J.A. (2021). Sulprostone-Induced Gastric Dysrhythmia in the Ferret: Conventional and Advanced Analytical Approaches. Frontiers in Physiology, 11, 583082.

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Chimney Model for Wound Healing

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For the wound-healing study, we used a chimney model mimicking the human wound-healing process [18 (link),21 (link)]. This chimney model was established in Balb/c nude mice (Orient Bio Inc., Gyeonggi-do, Korea), as described in a previous study [18 (link)]. Briefly, mice were anesthetized with an intraperitoneal injection of 40 μL of a solution containing ketamine (10 mg/kg; Ketamine, Yuhan Co., Seoul, Korea) and xylazine (40 mg/kg; Rompun®, Bayer, Leverkusen, Germany). After disinfecting each mouse by swabbing with alcohol, an excisional wound was made at the middle of the dorsal surface by using a 10 mm biopsy punch (Acuderm Inc., Fort Lauderdale, FL, USA). Thereafter, a chimney and 3D-printed artificial skin were inserted into the excisional wound. Subsequently, the chimney opening was sealed using a transparent film (Opsite, Smith & Nephew, London, UK) to prevent the drying and entry of external substrates.

Jang K.S., Park S.J., Choi J.J., Kim H.N., Shim K.M., Kim M.J., Jang I.H., Jin S.W., Kang S.S., Kim S.E, & Moon S.H. (2021). Therapeutic Efficacy of Artificial Skin Produced by 3D Bioprinting. Materials, 14(18), 5177.

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Immediate Prepectoral Implant Reconstruction

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All patients underwent a skin-sparing or skin-reducing mastectomy with immediate prepectoral implant reconstruction. The incision depended on the patient’s breast volume and degree of ptosis and included inframammary fold, Wise pattern, and periareolar incisions. The prepectoral implant was supported by one of a variety of ADMs including Strattice (Allergan, Ireland), Artia (Allergan, Ireland), and Surgimend (Integra LifeSciences) sutured to the anterior chest wall with 3-0 PDS (Polydioxanone) (Ethicon, UK). All incisions were closed with absorbable 3-0 Monocryl sutures (Ethicon, UK) for the dermis and 4-0 Monocryl for the subcuticular skin closure. The wounds were then glued with Dermabond skin adhesive (Ethicon, UK). NPWT was applied according to the manufacturer’s instructions (PICO, Smith & Nephew, UK), with size of dressing matched to wound size. Standard dressings were an Opsite (Smith & Nephew, UK) transparent waterproof dressing with an absorbent pad. Figure 1 shows the application of the PICO dressing in theatre.

Irwin G.W., Boundouki G., Fakim B., Johnson R., Highton L., Myers D., Searle R, & Murphy J.A. (2020). Negative Pressure Wound Therapy Reduces Wound Breakdown and Implant Loss in Prepectoral Breast Reconstruction. Plastic and Reconstructive Surgery Global Open, 8(2), e2667.

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Fabrication of Soft Bioelectronic Sensor

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The fabrication process of BCTS starts with preparation of sacrificial layer of polyimide (PI, ZKPI-3051), in which it is spinning coated on the silicon wafer with the thickness of ~1.5 μm, and cured at the temperature of 80 °C for 10 min, 120 °C for 10 min and 140 °C for 30 min. On the PI covered silicon wafer Cr (10 nm)/Au (100 nm) are deposited sequentially by sputtering, in which Cr acts as transition layer and Au the material in the function layer. Then etch Au/Cr into designed patterns by photolithography with the photoresist (PR, AZ5214E) as mask. The semipermeable film (Opsite, Smith & Nephew) is conformed to the patterned wafer as soft targeting substrate before transfer printing in solution. After transfer printing, the patterned ultra-thin metal film is integrated with the soft targeting substrate. Then CNT film ribbons are used as the extraction wire connected with the extraction pad. Finally, encapsulating the sensor with another layer of SF finishes the fabrication process.

Chen Y., Lu B., Chen Y, & Feng X. (2015). Breathable and Stretchable Temperature Sensors Inspired by Skin. Scientific Reports, 5, 11505.

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Chick Embryo Blood Volume Analysis

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In all investigations, chick embryos at d16 were used because at this time point the blood volume approaches the maximum during the incubation period21 (link). After precooling, the shell access windows were enlarged without hurting the CAM to facilitate the injection of the CA. A Cold Light Source, KL 1500 LCD (SCHOTT, Mainz, Germany) was used for illumination. 30 G 1/2 needles (BD Microlance^TM 3, BD Drogheda, Ireland) and 0.01–1 ml injectors (B|BRAUN, Melsungen, Germany) were used for injection. CA was injected into a chorioallantoic capillary vein of medium size. After 5 minutes hemostasis by compression with cotton swab, about 200 μl OpSite* (Smith & Nephew, London, England) was applied to stop bleeding completely after injection. After ensuring that the bleeding was totally controlled, eggs were put back into the refrigerator.

Zuo Z., Syrovets T., Wu Y., Hafner S., Vernikouskaya I., Liu W., Ma G., Weil T., Simmet T, & Rasche V. (2017). The CAM cancer xenograft as a model for initial evaluation of MR labelled compounds. Scientific Reports, 7, 46690.

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Wound Healing in Immunodeficient Mice

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The present study utilized immunodeficient Balb/c nude mice to suppress any immune responses to the transplanted human cells. All mice were anesthetized with intraperitoneal injection of 40 μL mixture containing rompun (40 mg/kg) and ketamine (10 mg/kg). Subsequently, a 12 mm excisional wound was cut on the middle of the dorsal surface by using a biopsy punch (Acuderm Inc., Fort Lauderdale, FL), and the differently conditioned scaffolds were transplanted onto the wound site, followed by covering with a transparent film (Opsite; Smith & Nephew, Andover, MA, USA) to prevent drying, detachment, and contamination. The transparent film is highly extensible and conformable with good moisture vapor permeability, allowing comfortable dressing during the entire experiment. We replaced the transparent film every 7 days to ensure better performance of the film. All experiments were approved by the animal care committee of Konkuk University (IACUC No. KU15151-1), and we performed the all animal experiment with the relevant guidelines and regulations of Konkuk IACUC.

Ko U.H., Choi J., Choung J., Moon S, & Shin J.H. (2019). Physicochemically Tuned Myofibroblasts for Wound Healing Strategy. Scientific Reports, 9, 16070.

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Circular Full-Thickness Skin Excision and Wound Healing

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After induction of general anesthesia with intramuscular injection of 40- to 50-mg/kg ketamine hydrochloride and 10-mg/kg xylazine hydrochloride, the surgical area was shaved and cleaned with PI solution. Using serrated forceps and iris scissors, a 2.5-cm diameter full-thickness circular piece of tissue was excised from the dorsal skin. Silicone splints were adhered and sutured to the wound perimeter to prevent wound contraction (Fig. 1). The wounds were treated with Betafoam and other control dressings, then covered with a transparent occlusive dressing (Opsite, Smith & Nephew). The dressing was changed every 2 to 3 days and the wound diameter was measured on postwounding days 3, 7, 10, and 14. The wound temperature, humidity, and odor were evaluated on days 3, 7, 10, and 14.

Lee J.W, & Song K.Y. (2017). Evaluation of a polyurethane foam dressing impregnated with 3% povidone-iodine (Betafoam) in a rat wound model. Annals of Surgical Treatment and Research, 94(1), 1-7.

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