Primary antibodies against gfap

THP (purity ≥99.0%) was purchased from Shanghai Selleck Chemicals (Shanghai, China). CFA and lipopolysaccharides were purchased from Sigma (St. Louis, United States). Primary antibodies against GFAP, Iba-1, NF-κB, p-NF-κB and GAPDH were purchased from Abcam (Cambridge, UK). All secondary antibodies conjugated with horseradish peroxidase (HRP) were purchased from Beyotime (Shanghai, China). Fetal bovine serum was purchased from Gibco (St. Louis, United States). Dulbecco’s modified Eagle’s medium (DMEM) was purchased from Corning (Manassas, USA). All of the chemicals and reagents used were of standard biochemical quality.^{12 (link)}

The Cornu Ammonis (CA) 1 and CA 3 regions of hippocampus were selected for the investigations of hippocampus. The brains of rats were removed and stored for 3 days in 10% formaldehyde in 0.1 M phosphate-buffer saline. The histopathological examinations were performed according to previous descriptions.^{32 (link)} GFAP immunohistochemistry was performed by primary antibodies against GFAP (Abcam, Inc., Mass, USA; 1/1000) for 24 hours. Olympus BX51 microscope was used for obtaining the histopathological images. GFAP immunostaining index was calculated as follows: GFAP-positive cells were counted at 40× magnification in randomized sections (3-4) for each rat. An imaging system (Image-Pro Express 1.4.5, Media Cybernetics, Inc. USA) was used for the assessments.^{8 (link),33 (link)}