One part of the aliquot was centrifuged at 1500×g at 4°C for 15 minutes and the supernatant was collected for the assessment of TGs, TC and FFAs using Synchron CX5 auto-analyzer (Beckman Instruments INC, Brea, USA).
Synchron cx5 auto analyzer
Manufactured by Beckman Coulter
Sourced in United States
The Synchron CX5 auto-analyzer is a clinical chemistry analyzer designed for automated testing of a wide range of analytes in biological samples. It is capable of performing multiple assays simultaneously, providing efficient and accurate results for clinical laboratories.
Automatically generated - may contain errors
3 protocols using synchron cx5 auto analyzer
1
Lipid Quantification in Centrifuged Samples
2
COVID-19 Biomarker Profiling in Blood
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Venous blood samples (4 mL) were obtained from all persons who tested positive to a COVID-19 PCR test and were divided into two aliquots: one aliquot was anticoagulated and divided into two test tubes. The first one was for hematological examinations of total leucocytes count (WBCs) using a Hema Screen 18-Automated Haematology Analyser (Hospitex Diagnostics, Sesto Fiorentino, Italy). The second test tube contained plasma separated for d -dimer with an Sysmex® CA-7000 system coagulation analyzer (Sysmex, Kobe, Japan), and detection of hs-C- reactive protein (CRP) with a Hitachi Model 7600 Series Automatic Analyzer (Hitachi High Technologies Corporation, Hitachi, Japan). The kits used in the experiments were d -dimer PLUS (Siemens Healthcare Diagnostics Products GmbH) and reagent kit for hs-CRP test (latex agglutination assay). Their reference values were 0.1417 (90% CL 0.00–0.55) µg/mL for d -dimer, and < 6 mg/L for hs-CRP, respectively.
From other aliquots of blood, samples could clot, and sera were then separated by centrifugation (3,500 rpm, 20 min, 25 °C) and stored at −20 °C for later biochemical determinations (serum creatinine and lactate dehydrogenase (LDH)). Plasma levels of total cholesterol, LDL-C, HDL-C, triacyclglycerols, and phospholipids were estimated using Synchron cx5 autoanalyzer (Beckman, USA) and calculated with the Friedewald formula.
From other aliquots of blood, samples could clot, and sera were then separated by centrifugation (3,500 rpm, 20 min, 25 °C) and stored at −20 °C for later biochemical determinations (serum creatinine and lactate dehydrogenase (LDH)). Plasma levels of total cholesterol, LDL-C, HDL-C, triacyclglycerols, and phospholipids were estimated using Synchron cx5 autoanalyzer (Beckman, USA) and calculated with the Friedewald formula.
3
Comprehensive Metabolic and Oxidative Profiling
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At the time of carnage, animals were weighed and blood was collected from the tail vein under brief ether anesthesia and was centrifuged (700×g, 4°C, 20 min) to separate sera. Sera were used to determine glucose, liver enzymes (AST and ALT) and malondialdehyde (MDA) as a measure for lipid peroxidation, using available colorimetric reagent kits [Randox (Antrim, U.K), Quimica Clinica Aplicada (Amposta, Spain) and Biovision (California, USA), respectively]. ELISA technique was carried out using the corresponding ELISA kit for the assessment of fructosamine (EIA ab, Wuhan, China), reduced glutathione (Cayman Chemical, Michigan, USA), adiponectin (Chemicon, MA, USA), the soluble receptor of advanced glycated end product (sRAGE) and visfatin (Ray Biotech, Georgia USA). Serum lipid profile, viz., triglycerides (TGs), total cholesterol (TC) and free fatty acids (FFAs) were estimated using Synchron CX5 auto-analyzer (Beckman Instruments INC, Brea, USA). Finally, insulin level was measured using rat RIA kit (Sceti Medical Labo K.K, Tokyo, Japan), and Homeostasis Model Assessment-index (HOMA-index) was calculated according to the following equation [Fasting glucose (mg/dl)/18×Fasting insulin (µIU/ml)]/22.5 [18] (link).
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