Cells were fixed in 4% PFA diluted in PBS and kept overnight at 4°C. The samples were quenched with 50 mM NH4Cl in PBS for 10 min at room temperature and permeabilized with 0.3% Triton X-100 and 5% FBS in PBS for 30 min. Antibodies were diluted in PBS containing 5% FBS and incubated for 1 h at room temperature. Between antibodies, cells were washed three times in PBS. Nuclei were stained for 10 min with DAPI (D1306; Thermo Fisher Scientific) diluted 1:10,000 in PBS. Coverslips were mounted on glass slides with DAKO mounting medium (DAKO, S3023). Antibodies used were CAT (D4P7B) XP (12980, 1:700; Cell Signaling), PEX14 (ab183885, 1:400; Abcam), PMP70 (ab211533, 1:400; Abcam), Tom20 (#11802-1-AP, 1:100; Invitrogen), OCT3/4 (SC5279, 1:100; Santa Cruz), TRA-1-60 (#MAB4360, 1:100; Millipore), TRA-1-81 (#MAB4381. 1:100; Millipore), anti-rabbit-Alexa Fluor 488 (A11034, 1:500; Life Technologies), Donkey anti-mouse IgG-594 (A21203, 1:300; Thermo Fisher Scientific), and anti-mouse Alexa Fluor 546 (A11003, 1:500; Life Technologies).
Cat d4p7b xp
Manufactured by Cell Signaling Technology
The CAT (D4P7B) XP is a primary antibody that specifically recognizes the CAT (Catalase) protein. It is suitable for use in various immunodetection applications, such as Western blotting, to identify and quantify the CAT protein in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Anti mouse alexa fluor 546, by Thermo Fisher Scientific (2 mentions)
D1306, by Thermo Fisher Scientific (2 mentions)
Tra 1 81, by Merck Group (2 mentions)
Oct3 4, by Santa Cruz Biotechnology (2 mentions)
Ab183885, by Abcam (2 mentions)
Mab4360, by Merck Group (2 mentions)
Dako mounting medium, by Agilent Technologies (2 mentions)
Alexa fluor 488 anti rabbit, by Thermo Fisher Scientific (2 mentions)
Pex14, by Abcam (2 mentions)
Pmp70, by Abcam (2 mentions)
2 protocols using cat d4p7b xp
1
Immunofluorescence Imaging of Cells
2
Immunofluorescence Imaging of Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cells were fixed in 4% PFA diluted in PBS and kept overnight at 4°C. The samples were quenched with 50 mM NH4Cl in PBS for 10 min at room temperature and permeabilized with 0.3% Triton X-100 and 5% FBS in PBS for 30 min. Antibodies were diluted in PBS containing 5% FBS and incubated for 1 h at room temperature. Between antibodies, cells were washed three times in PBS. Nuclei were stained for 10 min with DAPI (D1306; Thermo Fisher Scientific) diluted 1:10,000 in PBS. Coverslips were mounted on glass slides with DAKO mounting medium (DAKO, S3023). Antibodies used were CAT (D4P7B) XP (12980, 1:700; Cell Signaling), PEX14 (ab183885, 1:400; Abcam), PMP70 (ab211533, 1:400; Abcam), Tom20 (#11802-1-AP, 1:100; Invitrogen), OCT3/4 (SC5279, 1:100; Santa Cruz), TRA-1-60 (#MAB4360, 1:100; Millipore), TRA-1-81 (#MAB4381. 1:100; Millipore), anti-rabbit-Alexa Fluor 488 (A11034, 1:500; Life Technologies), Donkey anti-mouse IgG-594 (A21203, 1:300; Thermo Fisher Scientific), and anti-mouse Alexa Fluor 546 (A11003, 1:500; Life Technologies).
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